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Leuk Res ; 35(8): 1102-7, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21511336

ABSTRACT

The purpose of this study was to investigate the in vitro effects of thalidomide on long-term bone marrow cultures from patients with myelodysplastic syndrome. We demonstrated that thalidomide induced an increase in granulocyte-macrophage colony forming unit numbers and in IL-10 expression. Thalidomide also promoted a slight increase in IL-6, IL-1ß and TNF-α expression in the stromal layers. The numbers of erythroid burst forming units, the apoptosis rate of hematopoietic cells, and VEGF and TNF-α expression levels in culture supernatants were not modulated. Our results indicate a participation of thalidomide upon the hematopoietic microenvironment of patients with myelodysplastic syndromes, especially in the up regulation of IL-10.


Subject(s)
Angiogenesis Inhibitors/therapeutic use , Bone Marrow/drug effects , Interleukin-10/metabolism , Myelodysplastic Syndromes/drug therapy , Myelodysplastic Syndromes/metabolism , Stromal Cells/drug effects , Thalidomide/therapeutic use , Adult , Aged , Coculture Techniques , Colony-Forming Units Assay , Enzyme-Linked Immunosorbent Assay , Female , Hematopoietic Stem Cells , Humans , Interleukin-10/genetics , Interleukin-1beta/genetics , Interleukin-1beta/metabolism , Interleukin-6/genetics , Interleukin-6/metabolism , Male , Middle Aged , Myelodysplastic Syndromes/genetics , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , Stromal Cells/metabolism , Tumor Necrosis Factor-alpha/genetics , Tumor Necrosis Factor-alpha/metabolism , Vascular Endothelial Growth Factor A/genetics , Vascular Endothelial Growth Factor A/metabolism
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