Complete genome sequence of the Exiguobacterium bacteriophage.

We purified a lytic bacteriophage from soil collected in Guasave, Sinaloa: phiExGM16. This bacteriophage was isolated using the host, Exiguobacterium acetilycum. Its 17.6 kb genome contains 33 putative genes and shows a cover of 64% with 76.37% of nucleotide identity to Microbacterium phage Noelani.

Complete genome sequence of the Microbacterium enclense bacteriophage phiMiGM15.

We characterized the complete genome sequence of phiMiGM15, a lytic bacteriophage with siphovirus morphology that infects Microbacterium enclense. Its 48.6 kb genome contains 81 putative genes and shows coverage of 28% with 82.26% of nucleotide identity to Microbacterium phage Caron accession number OQ190481.1.

Cellulolytic Aerobic Bacteria Isolated from Agricultural and Forest Soils: An Overview.

This review provides insights into cellulolytic bacteria present in global forest and agricultural soils over a period of 11 years. It delves into the study of soil-dwelling cellulolytic bacteria and the enzymes they produce, cellulases, which are crucial in both soil formation and the carbon cycle. Forests and agricultural activities are significant contributors to the production of lignocellulosic biomass. Forest ecosystems, which are key carbon sinks, contain 20-30% cellulose in their leaf litter. Concurrently, the agricultural sector generates approximately 998 million tons of lignocellulosic waste annually. Predominant genera include Bacillus, Pseudomonas, Stenotrophomonas, and Streptomyces in forests and Bacillus, Streptomyces, Pseudomonas, and Arthrobacter in agricultural soils. Selection of cellulolytic bacteria is based on their hydrolysis ability, using artificial cellulose media and dyes like Congo red or iodine for detection. Some studies also measure cellulolytic activity in vitro. Notably, bacterial cellulose hydrolysis capability may not align with their cellulolytic enzyme production. Enzymes such as GH1, GH3, GH5, GH6, GH8, GH9, GH10, GH12, GH26, GH44, GH45, GH48, GH51, GH74, GH124, and GH148 are crucial, particularly GH48 for crystalline cellulose degradation. Conversely, bacteria with GH5 and GH9 often fail to degrade crystalline cellulose. Accurate identification of cellulolytic bacteria necessitates comprehensive genomic analysis, supplemented by additional proteomic and transcriptomic techniques. Cellulases, known for degrading cellulose, are also significant in healthcare, food, textiles, bio-washing, bleaching, paper production, ink removal, and biotechnology, emphasizing the importance of discovering novel cellulolytic strains in soil.

Complete Genome Sequence of Xanthomonas vesicatoria Bacteriophage ΦXaF18, a Contribution to the Biocontrol of Bacterial Spot of Pepper in Mexico.

Bacteriophage ΦXaF18 infects Xanthomonas vesicatoria, which is the causal agent of bacterial spot in tomato (Solanum lycopersicum L.) and pepper (Capsicum annuum L.). In this announcement, we present the complete genome of X. vesicatoria bacteriophage ΦXaF18, a 47,407-bp genome with 67 protein-coding genes.

Complete Genome Sequence of XaF13, a Novel Bacteriophage of Xanthomonas vesicatoria from Mexico.

The phytopathogenic bacterium Xanthomonas vesicatoria is the causative agent of bacterial spot disease in various Solanaceae family members. Here, we describe the complete genome sequence of XaF13, a novel filamentous phage that infects the phytopathogenic bacterium X. vesicatoria The 7,045-bp genome is predicted to encode 14 open reading frames, 7 of which are related to those of other filamentous Xanthomonas phages.