Shelf life extension of mozzarella cheese contaminated with Penicillium spp. using the antifungal compound ɛ-polylysine.

Molds are one of the most important spoilage organisms on cheese which can lead to economic loss as well as raising public health concerns due to the production of mycotoxins. This study investigates the use of ɛ-polylysine as natural antimicrobial to inhibit fungal growth. The minimal inhibitory concentrations and minimal fungicidal concentrations of ɛ-polylysine were determined against Penicillium roqueforti, Penicillium nordicum, and Penicillium solitum. Then, polylysine was tested as surface antimicrobial for the preservation of mozzarella slice cheese inoculated with these Penicillium spp. and stored in plastic trays during 25 days. The minimal inhibitory concentrations calculated for the three fungi tested were of 60 mg/l whereas the minimal fungicidal concentrations detected were of 125-10,000 mg/l. The shelf life observed for the control experiments was of 15 days, and just using the ɛ-polylysine at 0.00625, 0.0125, and 0.025% was evidenced a shelf life increment in comparison with the control of 1-3 days.

[Evaluation of the effect of hospital clown's performance about anxiety in children subjected to surgical intervention]. / Evaluación del efecto de la actuación de los payasos de hospital sobre la ansiedad, en los niños sometidos a una intervención quirúrgica.

OBJECTIVE: To be hospitalized is a highly distressing event for children. At present, a resort used in Spain and other countries to reduce children's anxiety in the health context are hospital's clown. We studied the effect of the hospital's clowns about the anxiety in children that going to be operated. METHODS: We recruited 60 children aged 6 to 10 years scheduled to undergo elective surgery. 30 children would have clowns before the surgery (case group) and 30 would not have them (control group). In the case group, two clowns performed for children. We measured the anxiety with several scales (STAIC, CCPH, faces scale), after the performance and until 7 days after the surgery. RESULTS: The outcomes show both groups a tendency to increase anxiety but the children of the case group showed less increase at the anxiety's score. In the control group is showed that the children are more alterated at seven days from the discharge. CONCLUSIONS: Children that receive the clown's care, have tendency to be less distressing and with less fear that another ones, measurement by STAIC and faces scale, and these results are maintained seven days after the discharge.

[The impact of psychological preparation for pediatric surgery on postoperative recovery]. / Repercusión de la preparación psicológica a la cirugía pediátrica sobre la recuperación postquirúrgica.

OBJECTIVE: Fear of surgery affects recovery in the emotional and behavioral state that leads the child to call the nurse more often or to ingest more sedatives after the operation. The present work evaluated the effects of psychological preparation for surgery during the pre-surgical period on the post-surgical recovery during the hospital stay in order to evaluate the effects that this preparation had on the recovery of the child. PATIENTS AND METHODS: The sample was composed of 60 pediatric patients of both sexes between 7 and 14 years of age admitted for minor scheduled surgery. Fear and five indicators of recovery were evaluated during the post-surgical period (call to the nurse, ingestion of sedatives and liquids, miction and sleep). The children were assigned randomly to one of the following four groups: filmed modeling, coping skills, filmed modeling plus coping skills, and control. RESULTS: The results indicated that the prepared children showed less fear, called the nurses less often, were administered fewer doses of sedatives and slept better after the operation. Analysis of the size of the effect showed that the programs that included coping skills training obtained the most benefits in the post-surgical recovery.