ABSTRACT
Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets. As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively. Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species. Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment.
ABSTRACT
La macroamilasemia debe sospecharse cuando un paciente presenta una concentración catalítica elevada de la α-amilasa plasmática, sin la presencia de datos clínicos que confirmen la existencia de una enfermedad pancreática o parotídea. La macroamilasemia, en la mayoría de los casos, es un complejo formado por la α-amilasa y las inmunoglobulinas plasmáticas que causa una hiperamilasemia con la amilasuria normal o baja. El diagnóstico diferencial con las otras causas de hiperamilasemia es imprescindible para evitar las exploraciones complementarias y los tratamientos invasivos innecesarios. Se presenta el caso de un varón de 53 años con dolor abdominal e hiperamilasemia, diagnosticado inicialmente de pancreatitis aguda. Macroamylasaemia should be suspected when a patient has a high catalytic concentration of plasma α-amylase in the absence of clinical data confirming the existence of a pancreatic or parotid disease (AU)
In most cases, macroamylasaemia is a complex of α-amylase bound to plasma immunoglobulins that cause hyperamylasaemia with low or normal urine amylase. The differential diagnosis with other causes of hyperamylasaemia is essential to avoid unnecessary additional examinations and invasive treatments. The case is presented of a 53 year-old male with abdominal pain and a high plasma amylase, initially diagnosed with acute pancreatitis (AU)
