Buckling of lipidic ultrasound contrast agents under quasi-static load.

Collapse of lipidic ultrasound contrast agents under high-frequency compressive load has been historically interpreted by the vanishing of surface tension. By contrast, buckling of elastic shells is known to occur when costly compressible stress is released through bending. Through quasi-static compression experiments on lipidic shells, we analyse the buckling events in the framework of classical elastic buckling theory and deduce the mechanical characteristics of these shells. They are then compared with that obtained through acoustic characterization. This article is part of the theme issue 'Probing and dynamics of shock sensitive shells'.

Embryo-scale epithelial buckling forms a propagating furrow that initiates gastrulation.

Cell apical constriction driven by actomyosin contraction forces is a conserved mechanism during tissue folding in embryo development. While much is now understood of the molecular mechanism responsible for apical constriction and of the tissue-scale integration of the ensuing in-plane deformations, it is still not clear if apical actomyosin contraction forces are necessary or sufficient per se to drive tissue folding. To tackle this question, we use the Drosophila embryo model system that forms a furrow on the ventral side, initiating mesoderm internalization. Past computational models support the idea that cell apical contraction forces may not be sufficient and that active or passive cell apico-basal forces may be necessary to drive cell wedging leading to tissue furrowing. By using 3D computational modelling and in toto embryo image analysis and manipulation, we now challenge this idea and show that embryo-scale force balance at the tissue surface, rather than cell-autonomous shape changes, is necessary and sufficient to drive a buckling of the epithelial surface forming a furrow which propagates and initiates embryo gastrulation.

Collective orientation of an immobile fish school and effect on rheotaxis.

We study the orientational order of an immobile fish school. Starting from the second Newton law, we show that the inertial dynamics of orientations is ruled by an Ornstein-Uhlenbeck process. This process describes the dynamics of alignment between neighboring fish in a shoal-a dynamics already used in the literature for mobile fish schools. First, in a fluid at rest, we calculate the global polarization (i.e., the mean orientation of the fish), which decreases rapidly as a function of noise. We show that the faster a fish is able to reorient itself the more the school can afford to reorder itself for important noise values. Second, in the presence of a stream, each fish tends to orient itself and swims against the flow: so-called rheotaxis. So, even in the presence of a flow, it results in an immobile fish school. By adding an individual rheotaxis effect to alignment interaction between fish, we show that in a noisy environment individual rheotaxis is enhanced by alignment interactions between fish.

Spherical oscillations of encapsulated microbubbles: Effect of shell compressibility and anisotropy.

We introduce a model that describes spherical oscillations of encapsulated microbubbles in an unbounded surrounding fluid. A Rayleigh-Plesset-like equation is derived by coupling the Navier-Stokes equation that describes fluid dynamics with the Navier equation that describes solid dynamics via the internal/external boundary conditions. While previous models were restricted to incompressible isotropic shells, the solid shell is modeled here as a compressible viscoelastic isotropic material and then generalized to an anisotropic material. The exact value of the resonance frequency is calculated analytically, and the damping constant is computed in the approximation of weak damping. A correction of the widely used Church model for incompressible shells is evidenced, and the effects of shell compressibility and anisotropy are discussed.

Let's deflate that beach ball.

We investigate the relationship between pre-buckling and post-buckling states as a function of shell properties, within the deflation process of shells of an isotropic material. With an original and low-cost set-up that allows to measure simultaneously volume and pressure, elastic shells whose relative thicknesses span on a broad range are deflated until they buckle. We characterize the post-buckling state in the pressure-volume diagram, but also the relaxation toward this state. The main result is that before as well as after the buckling, the shells behave in a way compatible with predictions generated through thin shell assumption, and that this consistency persists for shells where the thickness reaches up to 0.3 the shell's midsurface radius.

Asymmetric adhesion of rod-shaped bacteria controls microcolony morphogenesis.

Surface colonization underpins microbial ecology on terrestrial environments. Although factors that mediate bacteria-substrate adhesion have been extensively studied, their spatiotemporal dynamics during the establishment of microcolonies remains largely unexplored. Here, we use laser ablation and force microscopy to monitor single-cell adhesion during the course of microcolony formation. We find that adhesion forces of the rod-shaped bacteria Escherichia coli and Pseudomonas aeruginosa are polar. This asymmetry induces mechanical tension, and drives daughter cell rearrangements, which eventually determine the shape of the microcolonies. Informed by experimental data, we develop a quantitative model of microcolony morphogenesis that enables the prediction of bacterial adhesion strength from simple time-lapse measurements. Our results demonstrate how patterns of surface colonization derive from the spatial distribution of adhesive factors on the cell envelope.

Buckling Instability Causes Inertial Thrust for Spherical Swimmers at All Scales.

Microswimmers, and among them aspirant microrobots, generally have to cope with flows where viscous forces are dominant, characterized by a low Reynolds number (Re). This implies constraints on the possible sequences of body motion, which have to be nonreciprocal. Furthermore, the presence of a strong drag limits the range of resulting velocities. Here, we propose a swimming mechanism which uses the buckling instability triggered by pressure waves to propel a spherical, hollow shell. With a macroscopic experimental model, we show that a net displacement is produced at all Re regimes. An optimal displacement caused by nontrivial history effects is reached at intermediate Re. We show that, due to the fast activation induced by the instability, this regime is reachable by microscopic shells. The rapid dynamics would also allow high-frequency excitation with standard traveling ultrasonic waves. Scale considerations predict a swimming velocity of order 1 cm/s for a remote-controlled microrobot, a suitable value for biological applications such as drug delivery.

How a curved elastic strip opens.

An elastic strip is transversely clamped in a curved frame. The induced curvature decreases as the strip opens and connects to its flat natural shape. Various ribbon profiles are measured and the scaling law for the opening length validates a description where the in-plane stretching gradually relaxes the bending stress. An analytical model of the strip profile is proposed and a quantitative agreement is found with both experiments and simulations of the plates equations. This result provides a unique illustration of smooth nondevelopable solutions in thin sheets.

Statistical mechanics of two-dimensional shuffled foams: geometry-topology correlation in small or large disorder limits.

Bubble monolayers are model systems for experiments and simulations of two-dimensional packing problems of deformable objects. We explore the relation between the distributions of the number of bubble sides (topology) and the bubble areas (geometry) in the low liquid fraction limit. We use a statistical model [M. Durand, Europhys. Lett. 90, 60002 (2010)] which takes into account Plateau laws. We predict the correlation between geometrical disorder (bubble size dispersity) and topological disorder (width of bubble side number distribution) over an extended range of bubble size dispersities. Extensive data sets arising from shuffled foam experiments, surface evolver simulations, and cellular Potts model simulations all collapse surprisingly well and coincide with the model predictions, even at extremely high size dispersity. At moderate size dispersity, we recover our earlier approximate predictions [M. Durand, J. Kafer, C. Quilliet, S. Cox, S. A. Talebi, and F. Graner, Phys. Rev. Lett. 107, 168304 (2011)]. At extremely low dispersity, when approaching the perfectly regular honeycomb pattern, we study how both geometrical and topological disorders vanish. We identify a crystallization mechanism and explore it quantitatively in the case of bidisperse foams. Due to the deformability of the bubbles, foams can crystallize over a larger range of size dispersities than hard disks. The model predicts that the crystallization transition occurs when the ratio of largest to smallest bubble radii is 1.4.

Gel phase vesicles buckle into specific shapes.

Osmotic deflation of giant vesicles in the rippled gel phase P(ß') gives rise to a large variety of novel faceted shapes. These shapes are also found from a numerical approach by using an elastic surface model. A shape diagram is proposed based on the model that accounts for the vesicle size and ratios of three mechanical constants: in-plane shear elasticity and compressibility (usually neglected) and out-of-plane bending of the membrane. The comparison between experimental and simulated vesicle morphologies reveals that they are governed by a typical elasticity length, of the order of 1   µm, and must be described with a large Poisson's ratio.

Statistical mechanics of two-dimensional shuffled foams: prediction of the correlation between geometry and topology.

We propose an analytical model for the statistical mechanics of shuffled two-dimensional foams with moderate bubble size polydispersity. It predicts without any adjustable parameters the correlations between the number of sides n of the bubbles (topology) and their areas A (geometry) observed in experiments and numerical simulations of shuffled foams. Detailed statistics show that in shuffled cellular patterns n correlates better with √A (as claimed by Desch and Feltham) than with A (as claimed by Lewis and widely assumed in the literature). At the level of the whole foam, standard deviations Δn and ΔA are in proportion. Possible applications include correlations of the detailed distributions of n and A, three-dimensional foams, and biological tissues.

Buckling resistance of solid shell bubbles under ultrasound.

Thin solid shell contrast agents bubbles are expected to undergo different volume oscillating behaviors when the acoustic power is increased: small oscillations when the shell remains spherical, and large oscillations when the shell buckles. Contrary to bubbles covered with thin lipidic monolayers that buckle as soon as compressed: the solid shell bubbles resist compression, making the buckling transition abrupt. Numerical simulations that explicitly incorporate a shell bending modulus give the critical buckling pressure and post-buckling shape, and show the appearance of a finite number of wrinkles. These findings are incorporated in a model based on the concept of effective surface tension. This model compares favorably to experiments when adjusting two main parameters: the buckling tension and the rupture shell tension. The buckling tension provides a direct estimation of the acoustic pressure threshold at which buckling occurs.

Ultra-fast underwater suction traps.

Carnivorous aquatic Utricularia species catch small prey animals using millimetre-sized underwater suction traps, which have fascinated scientists since Darwin's early work on carnivorous plants. Suction takes place after mechanical triggering and is owing to a release of stored elastic energy in the trap body accompanied by a very fast opening and closing of a trapdoor, which otherwise closes the trap entrance watertight. The exceptional trapping speed--far above human visual perception--impeded profound investigations until now. Using high-speed video imaging and special microscopy techniques, we obtained fully time-resolved recordings of the door movement. We found that this unique trapping mechanism conducts suction in less than a millisecond and therefore ranks among the fastest plant movements known. Fluid acceleration reaches very high values, leaving little chance for prey animals to escape. We discovered that the door deformation is morphologically predetermined, and actually performs a buckling/unbuckling process, including a complete trapdoor curvature inversion. This process, which we predict using dynamical simulations and simple theoretical models, is highly reproducible: the traps are autonomously repetitive as they fire spontaneously after 5-20 h and reset actively to their ready-to-catch condition.

Two-dimensional flow of foam around an obstacle: force measurements.

A Stokes experiment for foams is proposed. It consists of a two-dimensional flow of a foam, confined between a water subphase and a top plate, around a fixed circular obstacle. We present systematic measurements of the drag exerted by the flowing foam on the obstacle versus various separately controlled parameters: flow rate, bubble volume, bulk viscosity, obstacle size, shape, and boundary conditions. We separate the drag into two contributions: an elastic one (yield drag) at vanishing flow rate and a fluid one (viscous coefficient) increasing with flow rate. We quantify the influence of each control parameter on the drag. The results exhibit in particular a power-law dependence of the drag as a function of the bulk viscosity and the flow rate with two different exponents. Moreover, we show that the drag decreases with bubble size and increases proportionally to the obstacle size. We quantify the effect of shape through a dimensional drag coefficient, and we show that the effect of boundary conditions is small.