Impact of prolonged storm activity on the Ecological Status of intertidal benthic habitats within oyster (Crassostrea gigas) trestle cultivation sites.

The Ecological Status (ES; sensu the Water Framework Directive) of intertidal benthic communities within six oyster trestle cultivation sites was found to be negatively impacted along the access routes to trestles in a 2013 study. All cultivation sites occur within Natura 2000 sites. The current study revisited four of the 2013 cultivation sites in February 2014 one month after the storm activity of winter 2013/14 to test if the compaction effect along access routes persisted after the storms. Three levels of the fixed factor treatment were sampled; immediately below the trestles, along the access route and 300m away from any anthropogenic activity. The compaction effect at the Access treatment persisted in spite of the major storm activity. The current study showed the IQI to be effective for assessing the impacts of aquaculture and highlights the IQI as a tool for monitoring Conservation Status of intertidal communities under the Habitats Directive.

Expression patterns of conserved microRNAs in the male gametophyte of loblolly pine (Pinus taeda).

MicroRNAs (miRNAs) are small RNAs that regulate genes involved in various aspects of plant development, but their presence and expression patterns in the male gametophytes of gymnosperms have not yet been established. Therefore, this study identified and compared the expression patterns of conserved miRNAs from two stages of the male gametophyte of loblolly pine (Pinus taeda), which are the mature (ungerminated) and germinated pollen. Microarray was used to identify conserved miRNAs that varied in expression between these two stages of the loblolly pine male gametophyte. Forty-seven conserved miRNAs showed significantly different expression levels between mature and germinated loblolly pine pollen. In particular, miRNAs representing 14 and 8 families were up- and down-regulated in germinated loblolly pine pollen, respectively. qRT-PCR was used to validate their expression patterns using representative miRNAs. Target genes and proteins were identified using psRNATarget program. Predicted targets of the 22 miRNA families belong mostly to classes of genes involved in defense/stress response, metabolism, regulation, and signaling. qRT-PCR was also used to validate the expression patterns of representative target genes. This study shows that conserved miRNAs are expressed in mature and germinated loblolly pine pollen. Many of these miRNAs are differentially expressed, which indicates that the two stages of the male gametophyte examined are regulated at the miRNA level. This study also expands our knowledge of the male gametophytes of seed plants by providing insights on some similarities and differences in the types and expression patterns of conserved miRNAs between loblolly pine with those of rice and Arabidopsis.

An exploration of the spatial scale over which orientation-dependent surround effects affect contour detection.

Contour detection is a crucial component of visual processing; however, performance on contour detection tasks can vary depending on the context of the visual scene. S. C. Dakin and N. J. Baruch (2009) showed that detection of a contour in an array of distracting elements depends on the orientation of flanking elements. Here, using a line of five collinear Gabor elements ("target contour") in a field of distractor Gabor elements, we systematically measured the effects of eccentricity, spacing, and spatial frequency on contour detection performance in three different contexts: randomly oriented distractors (control condition), flanking distractors (on either side of the collinear Gabors) aligned approximately parallel to the target contour, and flanking distractors aligned approximately orthogonal to the target contour. In the control condition, contour detection performance was best for larger Gabors (2 cpd) spaced farther apart (1.2°). Parallel flankers reduced performance for intermediate and large spacings and sizes compared to the control condition, while orthogonal flankers increased performance for the smallest spacing and size compared to the control condition. The results are fit by a model in which collinear facilitation, which is size-dependent but can persist for several degrees of visual angle, competes with orientation-dependent suppression from the flanking context when elements are separated by less than a degree of visual angle.

Challenges and strategies of dyad research: cardiovascular examples.

This article highlights challenges in recruitment, retention, attrition, data collection, and analysis when studying dyads in cardiovascular research. Including family members in studies is particularly important because family members often have a major role in treatment of patients with cardiovascular illness. The partnership of a patient-family member is referred to as a dyad and may include the cardiovascular patient and another relative, such as an adult child, sibling, spouse, son-in-law or daughter-in-law, or unmarried partner. Insights gained from previous research may facilitate and improve rigor when reviewing and conducting studies involving dyads with cardiovascular and other chronic diseases. Including patients and partners in descriptive and intervention studies will allow researchers to more fully explore family factors that may be salient in health outcomes.

Heart failure symptom assessment and management: can caregivers serve as proxy?

BACKGROUND: : Caregivers (CGs) of heart failure (HF) patients are increasingly assuming greater responsibilities in symptom assessment, evaluation, and decision making and may be asked to serve as proxies. The purpose of this study was to examine the degree of congruence between HF patients and their primary CG on symptom assessment and self-care management behaviors. METHODS: : The sample consisted of 70 HF patients receiving home healthcare and their designated CG. Patients were primarily between 40 and 85 years of age and female (60%). Caregivers were predominately female (76%) and spouses (43%). Congruence in symptom assessment and management between HF patients and their designated CGs was measured in this descriptive cross-sectional study using the Heart Failure Symptom Survey and Self-care of Heart Failure Index. RESULTS: : The Spearman correlation coefficient and concordance correlation coefficient were used to assess the degree of congruence on symptom evaluation scores from the Heart Failure Symptom Survey. Dyads had the strongest correlations on ratings of the HF patients' symptoms of extremity edema, difficulty concentrating, and dizziness. Lower congruence (Spearman correlation <0.40) was found on feeling depressed, shortness of breath at night, and shortness of breath when lying down. Scores on the Self-care of Heart Failure Index self-care management and self-care confidence scales were not significantly different within the HF dyads. CONCLUSIONS: : Based on moderate levels of correlations on most HF symptoms in this study, the CG as proxy appears to be a reasonable substitute for patient responses in a community setting. However, greater family preparation for symptom assessment is warranted to improve congruence for future symptom assessment.

Use of the concordance correlation coefficient when examining agreement in dyadic research.

BACKGROUND: The statistical measure used to quantify the degree of agreement or congruence between two research subjects has been the intraclass or the Pearson correlation coefficient; however, the concordance correlation coefficient (CCC) is another measure of agreement used to examine agreement between two observers or raters. OBJECTIVES: The aims of this study were to (a) highlight the differences among three statistical measures used to quantify the degree of agreement or congruence, (b) demonstrate the use of the CCC in examining agreement between heart failure (HF) patients and their family members, and (c) provide nurse researchers another method for evaluating agreement. METHODS: Symptom evaluation scores obtained from HF patients and their family members were used in the analysis of this study. To explain the use of the CCC in this analysis, a distinction between Pearson correlation coefficient and intraclass correlation coefficient is discussed. The CCC calculation is then described in detail. RESULTS: The HF patients in this sample were 71 +/- 9.6 years in age, 40% male, and 41.4% African American. Most (75%) family members were female. There were several different categories of family members, but most were spouses. The CCC results indicated that no symptom achieved good agreement, and 8 of 14 symptoms were in moderate agreement (.4

Family influences on heart failure self-care and outcomes.

Many patient education guidelines for teaching heart failure patients recommend inclusion of the family; however, family-focused interventions to promote self-care in heart failure are few. This article reviews the state of the science regarding family influences on heart failure self-care and outcomes. The literature and current studies suggest that family functioning, family support, problem solving, communication, self-efficacy, and caregiver burden are important areas to target for future research. In addition, heart failure patients without family and those who live alone and are socially isolated are highly vulnerable for poor self-care and should receive focused attention. Specific research questions based on existing science and gaps that need to be filled to support clinical practice are posed.

Low-technology heart failure care in home health: improving patient outcomes.

This article reports a study that pilot tested the effectiveness of a low-technology structured intervention to standardize home healthcare management of patients with heart failure (HF) within a home health agency (HHA). The purpose of this study was to use low-technology equipment to improve care for patients with HF enrolled in a home health agency. The 9-week intervention was targeted toward the home health nurses and included telephone and home visits, a teaching tool, digital scales, and a log/notebook filled out by the patients in the study. Patient outcomes included decreased rehospitalization, decreased symptoms of HF, and increased quality of life.

End-of-life care with the heart failure patient in home health: a case study.

The diagnosis of congestive heart failure accounts for a large proportion of hospital admissions and multiple readmissions over the course of the disease. During the end stage of their disease process, patients often are prescribed many complex medications such as inotropic infusion therapy. For a patient to receive an intravenous medication at home, there must be a collaborative approach between nurse, family, patient, and physician. This palliative approach can result in decreased hospital admissions and an improved quality of life for patients with end-stage congestive heart failure.