Plug-and-play adaptive optics for commercial laser scanning fluorescence microscopes based on an adaptive lens.

In this Letter, we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics are implemented by the introduction of a multiactuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom-made multiphoton excitation microscope.

Correction of non-common path aberrations in pyramid wavefront sensors to recover the optimal magnitude gain using a deformable lens.

Adaptive optics (AO) correction based on pyramid wavefront sensors (P-WFSs) has been successfully implemented in several instruments for astronomical observation due to the P-WFS advantages in terms of sensitivity with respect to other WFSs, such as the Shack-Hartmann. The correction of non-common path aberrations (NCPAs) between the sensing and the scientific arm, commonly performed introducing offsets to the Zernike coefficients of the measured wavefront in the AO closed loop, reduces the sensitivity of P-WFSs causing a loss in sky coverage and scientific throughput. We propose a technique to exploit the full capabilities of P-WFSs compensating the NCPAs up to the fourth order on the WFS channel by means of a multi-actuator adaptive lens (MAL). We show the preliminary results obtained in a dedicated laboratory test bench.

Optical characterization and adaptive optics correction of polymer adaptive lens aberrations.

Adaptive lenses based on fluid-filled polymer membranes allow for great simplification of optical systems providing large focal length variation and reduction of size, weight, and power consumption. However, aberrations can reduce their optical quality and, for some demanding applications, their correction by means of adaptive optics implies increased complexity, especially if reflective wavefront correctors are used. In this work, we characterize two adaptive lenses in terms of optical power and aberrations. We then correct the gravity-induced aberrations by means of a multiactuator adaptive lens in a closed-loop adaptive optics configuration, with a minimal increase in optical setup complexity. The improvements in the performance of an imaging system are shown.