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1.
Cell Microbiol ; 7(6): 849-67, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15888087

ABSTRACT

The intracellular protozoan Toxoplasma gondii lacks a de novo mechanism for cholesterol synthesis and therefore must scavenge this essential lipid from the host environment. In this study, we demonstrated that T. gondii diverts cholesterol from low-density lipoproteins for cholesteryl ester synthesis and storage in lipid bodies. We identified and characterized two isoforms of acyl-CoA:cholesterol acyltransferase (ACAT)-related enzymes, designated TgACAT1alpha and TgACAT1beta in T. gondii. Both proteins are coexpressed in the parasite, localized to the endoplasmic reticulum and participate in cholesteryl ester synthesis. In contrast to mammalian ACAT, TgACAT1alpha and TgACAT1beta preferentially incorporate palmitate into cholesteryl esters and present a broad sterol substrate affinity. Mammalian ACAT-deficient cells transfected with either TgACAT1alpha or TgACAT1beta are restored in their capability of cholesterol esterification. TgACAT1alpha produces steryl esters and forms lipid bodies after transformation in a Saccharomyces cerevisiae mutant strain lacking neutral lipids. In addition to their role as ACAT substrates, host fatty acids and low-density lipoproteins directly serve as Toxoplasma ACAT activators by stimulating cholesteryl ester synthesis and lipid droplet biogenesis. Free fatty acids significantly increase TgACAT1alpha mRNA levels. Selected cholesterol esterification inhibitors impair parasite growth by rapid disruption of plasma membrane. Altogether, these studies indicate that host lipids govern neutral lipid synthesis in Toxoplasma and that interference with mechanisms of host lipid storage is detrimental to parasite survival in mammalian cells.


Subject(s)
Cholesterol Esters/biosynthesis , Lipids/physiology , Sterol O-Acyltransferase/metabolism , Toxoplasma/metabolism , Amino Acid Sequence , Animals , Binding Sites , Cell Membrane/metabolism , Cells, Cultured , Cricetinae , Cricetulus , Endoplasmic Reticulum/enzymology , Humans , Isoenzymes/genetics , Isoenzymes/metabolism , Mice , Microscopy, Immunoelectron , Molecular Sequence Data , Mutation , Palmitates/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Sterol O-Acyltransferase/genetics , Toxoplasma/enzymology , Toxoplasma/genetics
2.
Mol Biochem Parasitol ; 138(1): 107-22, 2004 Nov.
Article in English | MEDLINE | ID: mdl-15500922

ABSTRACT

In mammalian cells, the main stored neutral lipids are triacylglycerol and cholesteryl esters, which are produced by two related enzymes, acyl-CoA:diacylglycerol acyltransferase (DGAT) and acyl-CoA:cholesterol acyltransferase (ACAT), respectively. Very little is known about the metabolism, intracellular storage and function of neutral lipids in many pathogenic lower eukaryotes. In this paper, we have characterized the activity of an important triacylglycerol synthetic enzyme in the protozoan Toxoplasma gondii. A full-length cDNA and gene encoding a T. gondii DGAT1-related enzyme were identified and designated TgDGAT1. The gene is composed of 15 exons and 14 introns, and encodes a protein with a predicted M(r) 63.5kDa, containing signature motifs characteristic of the DGAT1 family. The native protein migrates at 44kDa under reducing conditions. TgDGAT1 is an integral membrane protein localized to the parasite cortical and perinuclear endoplasmic reticulum, with the C-terminus oriented to the lumen of the organelle. When a Saccharomyces cerevisiae mutant strain lacking neutral lipid production is transformed with TgDGAT1 cDNA, a significant DGAT activity is reconstituted, resulting in triacylglycerol synthesis and biogenesis of cytosolic lipid inclusions, resembling lipid bodies in T. gondii. No production of steryl esters is observed upon TgDGAT1 expression in yeast. In contrast to human DGAT1 lacking fatty acid specificity, TgDGAT1 preferentially incorporates palmitate. Our results indicate that parasitic protozoa are also neutral lipid accumulators and illustrate the first example of the existence of a functional DGAT gene in an ancient eukaryote, demonstrating that diacylglycerol esterification is evolutionarily conserved.


Subject(s)
Acyltransferases/genetics , Acyltransferases/metabolism , Inclusion Bodies/physiology , Lipid Metabolism , Toxoplasma/enzymology , Triglycerides/metabolism , Amino Acid Sequence , Animals , CHO Cells , Cell Line , Cricetinae , Cricetulus , DNA, Complementary , Diacylglycerol O-Acyltransferase , Endoplasmic Reticulum/metabolism , Evolution, Molecular , Humans , Inclusion Bodies/genetics , Molecular Sequence Data , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Toxoplasma/genetics
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