[Method for determining prostaglandins in urine]. / Metod opredeleniia prostaglandinov v moche.

A method is described for estimation of prostaglandins in human urine. The prostaglandins were isolated from urine using Amberlite HAD-2 and fractionated on a column with silicic acid. For the fractionation of the samples specific markers were required for each column. Two methods of column chromatography were compared. The radioimmunoassay was shown to be an accurate and highly reliable method. The advantages of the method are that PGE2 is converted into PGF2 alpha using sodium borhydride and the latter is estimated in a reaction with the specific antiserum supplied with the Kit.

[Comparative characteristics of ribonuclease isolated from the lliver and pancreas of burned and intact rats]. / Sravnitel'naia kharakteristika ribonukleazy, vydelennoi iz pecheni i podzheludochnoi zhelezy obozhzhennykh i intaktnykh krys

Alkaline RNA-ase was isolated from the pancreas and liver of intact and burnt rats, and purified 1400 and 2000 times accordingly. The optimum of the enzyme activity was achieved at pH 7.6-7.8. Normal and post-burn RNA-ase biosynthesis was studied using radioamino acids (C14-lysine and C14-glycine). Burnt animals displayed a slowed down alkaline RNA-ase biosynthesis, both in the liver and in the pancreas. Ribonuclease "half-life periods" of the liver of intact rats was 65 min, whereas in that of the burnt animals it was prolonged to 100.7 min. The end-preparation activity of the liver and pancreas alkaline RNA-ase of the intact and burnt animals showed no significant difference.

Influence of anesthesia on the rate of insulin biosynthesis.

Rates of insulin and total pancreatic protein biosynthesis were investigated by means of 1-14C-glycine under thiopental-induced deep anesthesia and thalamonal-induced neuroleptanalgesia. Blood levels of insulin and 11-OCS were also investigated. It was shown that both anesthetics - thalamonal and thiopental - inhibited rates of insulin synthesis and decrease its secretion. Only thiopental reduced the 11-OCS blood level. Changes of rate of insulin synthesis were observed on the background of inhibition of biosynthesis of total pancreatic proteins. But despite the absence of specific changes in the rate of insulin biosynthesis as compared to total pancreatic protein synthesis under both types of anesthesia, deep thiopental-induced anesthesia resulted in more severe changes in the biosynthesis of hormone and total proteins neuroleptanalgesia, since the latter induced only slight inhibition of rate of its synthesis.