[Realization of a measurement module for determining pH value in perfusion cultures]. / Realisierung eines Messmoduls für die pH-Wert-Bestimmung in Perfusionskulturen.

For cultivating animal cells in bioreactors the maintenance of a fixed pH-value is of elementary importance. In a perfused cell culture system the pH-value is measured by electrochemical flow rate sensors. For the acceptance of a technical solution the integration in complex workplaces is necessary, thereby small size and low costs are the main features. For that a special measurement module was developed, consisting of measuring amplifier and microcontroller component with CAN-Bus-interface.

[Development of a CAN (controller area network) bus for modules of a perfused cell culture system]. / Entwicklung einer CAN-Bus-Kopplung für Module eines perfundierten Zellkultursystems.

For computer-aided data acquisition and automated running of experiments in cell cultivation reactors networking of all sensoric and actoric devices is required. A low cost and high performance solution to this demand can be found by using the widely established CAN (Controller Area Network) bus. If standard PCs running MS Windows are used to control this network special measures have to be taken to prevent loss of data caused by differences in the computing power of the PC on one hand and microcontroller-based devices on the other.

[Recording and control of culture parameters in perfused cell culture systems]. / Erfassung und Steuerung von Versorgungsparametern in perfundierten Zellkultursystemen.

For the cultivation of animal cells perfused cell culture systems are advantageous. The measurement of the process parameters for the check of the culture is difficult through the small volumes and dimensions. A developed measuring system allows the measuring of the parameters temperature, pH-value and dissolved oxygen.