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Ann Biol Clin (Paris) ; 68(4): 449-53, 2010.
Article in French | MEDLINE | ID: mdl-20650740

ABSTRACT

The discriminatory potency of variable number of tandem repeats (VNTR), based on 7 loci (MIRU 26, 27 and 5 ETRs A, B, C, D, E) was assayed on Mycobacterium bovis strains obtained from samples due to tuberculosis in two slaughterhouses in Algeria. The technique of MIRU-VNTR has been evaluated on 88 strains of M. bovis and one strain of M. caprea and shows 41 different profiles. Results showed that the VNTR were highly discriminatory with an allelic diversity of 0.930 when four loci (ETR A, B, C and MIRU 27) were highly discriminatory (h>0.25) and three loci (ETR D and E MIRU 26) moderately discriminatory (0.11

Subject(s)
Minisatellite Repeats/genetics , Mycobacterium bovis/genetics , Abattoirs , Algeria , Animals , Cattle/microbiology , Chromosome Mapping/methods , Genes, Bacterial , Genetic Variation , Meat/microbiology , Mycobacterium bovis/classification , Mycobacterium bovis/isolation & purification , Polymerase Chain Reaction
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