ABSTRACT
Genetic efficiency data of nine short tandem repeat (STR) loci were determined by multiplex PCR using fluorescently labeled primers and subsequent analysis by capillary electrophoresis (ABI 310). For each locus 7-14 alleles were detected. The combined matching probability is about 1 x 10(-11). No deviations from Hardy-Weinberg equilibrium were observed.
Subject(s)
DNA Fingerprinting/methods , Dental Enamel Proteins/genetics , Gene Frequency/genetics , Genetic Markers/genetics , Minisatellite Repeats/genetics , Polymerase Chain Reaction/methods , Alleles , Amelogenin , Austria , Black People/genetics , Discriminant Analysis , Electrophoresis, Capillary , Humans , Paternity , Reproducibility of Results , United States , White People/geneticsABSTRACT
Population genetic data of the short tandem repeat system FGA were determined by PCR analysis in two Austrian population samples, one population north of the Alps and one population south of the Alps. A total of 15 different alleles could be observed in 500 unrelated individuals. No significant differences were found between the phenotype frequencies in the two populations, as determined by R x C contingency test, so the populations could be pooled for further analysis. Both the single populations and the pooled population are in accordance with Hardy-Weinberg equilibrium. FGA proves to be very efficient for both stain analysis and paternity testing. The presented allele and genotype data allow the statistical interpretation of this system for Austrians.
Subject(s)
DNA/analysis , Genetic Markers/genetics , Genetics, Population , Repetitive Sequences, Nucleic Acid/genetics , Alleles , Austria , Child , DNA/genetics , DNA Fingerprinting , DNA Primers/chemistry , Female , Gene Frequency/genetics , Genotype , Humans , Male , Phenotype , Polymerase Chain ReactionABSTRACT
Allele- and genotype frequencies of the two short tandem repeat (STR) systems VWA and TH01 were determined in an Austrian population sample by polymerase chain reaction (PCR) analysis. A total of 9 alleles for VWA and 6 alleles for TH01 could be observed in a population of 278 (VWA) and 276 (TH01) individuals. Both systems are in accordance with Hardy-Weinberg equilibrium. They are highly informative for individualization from stain analysis and, in addition, they are very useful for paternity testing. The data presented here allow the statistical interpretation of PCR results for an Austrian population.
Subject(s)
Alleles , DNA/analysis , Minisatellite Repeats , Austria , DNA Primers/chemistry , Electrophoresis, Polyacrylamide Gel , Forensic Medicine/methods , Gene Frequency , Humans , Polymerase Chain Reaction , Polymorphism, Restriction Fragment LengthABSTRACT
Allele frequencies of the AmpFLP system APO B were determined in an Austrian population sample consisting of 210 unrelated Caucasian individuals living in the Salzburg region. A total of 25 different alleles could be observed. The allele distributions were in accordance with Hardy-Weinberg equilibrium. No new mutations were found in 184 meioses and seven "interalleles" and four alleles < 29 could be detected.
Subject(s)
Apolipoproteins B/genetics , Gene Frequency , White People/genetics , Alleles , Austria , DNA/analysis , Forensic Medicine/methods , Humans , Meiosis , Mutation , Polymerase Chain ReactionABSTRACT
The Pharmacia Multiphor II horizontal electrophoresis chamber is a widespread tool for analysis of PCR products in forensic casework. Up to date, however, there is no protocol for successfully running high-resolution denaturing PAGE (poly-acrylamide gel electrophoresis) on a horizontal electrophoresis chamber. We modified the electrophoresis conditions to make this possible.
Subject(s)
Electrophoresis, Polyacrylamide Gel/methods , Repetitive Sequences, Nucleic Acid/genetics , DNA , Humans , Polymerase Chain Reaction , Polymorphism, Genetic/geneticsABSTRACT
Allele frequencies of the two short tandem repeat (STR) systems F13B and CD4 were determined in an Austrian population sample by PCR analysis. A total of 6 alleles for F13B and 8 alleles for CD4 could be observed in a population of 216 (F13B) and 198 (CD4) unrelated individuals. No significant deviations from Hardy-Weinberg equilibrium were observed.
Subject(s)
Gene Frequency , Repetitive Sequences, Nucleic Acid/genetics , Austria , DNA , Humans , Polymorphism, GeneticABSTRACT
This paper describes the first case in Austria where a suspect was been convicted and condemned because of salivary traces found on an empty bottle which were successfully typed by PCR-DNA-analysis. This was the only evidence in that case. In contrast to cigarette butts, which are already relatively routine for PCR-DNA-typing, bottles found on crime scenes usually contain much less material which can be investigated. As the result of permanent refinement of the methods used, it is demonstrated that it should be possible to solve other similar cases in the future.
