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Clin Orthop Relat Res ; (394): 278-89, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11795745

ABSTRACT

The effect of standard orthopaedic implant materials on osteoblast proliferation and differentiation was investigated using a human osteoblast cell culture system. Human fetal osteoblasts 1.19 were cultured on stainless steel, cobalt-chrome-molybdenum, and commercially pure titanium for 12 days. Tissue culture polystyrene was used as a control. Cell proliferation was measured by electronic cell counting and by a colorimetric proliferation assay. To assess the degree of differentiation, levels of alkaline phosphatase activity, collagen Type I, and osteocalcin production were measured. Osteocalcin gene expression was measured by reverse transcriptase-polymerase chain reaction. Electronic cell counting and proliferation assays showed lower cell numbers and delayed proliferation on stainless steel and cobalt-chrome-molybdenum compared with titanium and polystyrene. Alkaline phosphatase and osteocalcin were measured higher on titanium than on stainless steel or cobalt-chrome-molybdenum. Differences in collagen Type I production were not found. Reverse transcriptase-polymerase chain reaction showed the highest osteocalcin gene expression on titanium. The human fetal osteoblast cell line 1.19 provides a rapidly proliferating and differentiating system for testing biomaterials in which differences in osteoblast proliferation and differentiation on orthopaedic implant materials could be revealed, suggesting that the chemistry of biomaterials has a dynamic effect on proliferation and differentiation of human osteoblasts.


Subject(s)
Alkaline Phosphatase/metabolism , Materials Testing/methods , Osteocalcin/metabolism , Prostheses and Implants , Analysis of Variance , Biocompatible Materials , Cell Division/physiology , Cells, Cultured , Female , Fetus , Humans , Osteoblasts/metabolism , Osteoblasts/physiology , Osteocalcin/genetics , Pregnancy , Probability , Reverse Transcriptase Polymerase Chain Reaction , Sensitivity and Specificity
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