Long-Term, High-Spatiotemporal Resolution Recording From Cultured Organotypic Slices with High-Density Microelectrode Arrays.

A novel system to cultivate and record brain slices directly on high-density microelectrode arrays (HD-MEA) was developed. This system allows to continuously record electrical activity of selected individual neurons at high spatial resolution, while monitoring neuronal network activity at the same time. For the first time, properties of single neurons and the corresponding neuronal network in an organotypic hippocampal slice culture were studied over four consecutive weeks at daily intervals.

[Neural manifestations in Lyme disease (Lyme borreliosis of the nervous system)]. / Nervne pojave u Lajmskoj bolesti (Lajm-borelioza nervnog sistema).

The involvement of the nervous system is common during Lyme's disease, and the term neuroborreliosis has been established. All structures of the nervous system, from meninges to periferial nerves, can be involved. Neurological manifestations are most common in the second stage (dissemination). The article deals with the most important neurological manifestations, as well as with the contemporary pathogenetic considerations and therapy. Eleven patients with neuroborreliosis who were treated at Dr. Kosta Todorovitsh Institute of Infectious and Tropical Diseases, are reviewed. Five of them had acute meningoencephalitis, of whom two had concurrent neuritis; one patient had Banawart's syndrome with arthralgias, arthritis and fatigue syndrome; two patients had neuritis; one had bilateral facial palsy; two had chronic fatigue syndrome.

The analysis of virus-caused alterations of cellular and humoral immunoresponsiveness during the first days of morbilli rush in the young adult population.

The parameters of cellular and humoral immune responsiveness were studied during the first days of morbilli rush in the group of 34 young adult patients. The analysis of cell-mediated immune response included T cell relative and absolute numbers, mitogen-induced T cell proliferative response, as well as the relative and absolute numbers of mononuclear phagocytes. The tested parameters of humoral immune response included B cell relative and absolute numbers, serum concentrations of immunoglobulins IgG, IgA and IgM, and serum level of immune complexes (IC). The reduction of total leukocyte number, T cell and B cell relative and absolute numbers, as well as diminished mitogen-induced T cell proliferative response, associated with the elevation of IgM and IC serum levels were found in the majority of analyzed patients. Moreover, the subsequent analysis revealed positive correlations between T cell percentage and T cell proliferative response, as well as between serum concentrations of IgM and IC. These data confirmed the presence of acute morbilli infection-caused disturbances of patients' immunocompetence.

Cross finger flap. A new technique.

Avulsion injuries of the thumb resulting in volar defects and loss of neurovascular bundles are presented. Treatment should aim at restoration of the skin and pulp with as near normal sensation as possible. A new technique is described using a cross-finger flap or flaps with transfer of the superficial terminal branch of the radial nerve. It is claimed that this gives near normal restoration of sensation to the tactile surface of the thumb. The fingers may be treated in the same way for similar injuries.