ABSTRACT
Let's go ChemBioTalents! ChemBioChem is excited to introduce its very first Early Career Advisory Board - packed with 15 exceptional early career researchers from all over the world.
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Advisory Committees , Career Choice , Research Personnel , HumansABSTRACT
Talent is our future! ChemBioChem wants to help strengthen the ties between chemistry and biology by further supporting young talented researchers, the future of this research area. Be sure not to miss our young researcher Issue ChemBioTalents!
ABSTRACT
The broader, the better! ChemBioChem had a successful year in 2017 receiving its 10 000 th submission. By broadening the journal scope through the addition of biomaterials, ChemBioChem will help to intertwine chemistry and biology even further. Also up next in 2018, a young chemists Special Issue, so gather up ChemBioTalents!
Subject(s)
Publishing , Peer Review, Research , Periodicals as TopicABSTRACT
Superoxide dismutases (SOD) are a group of enzymes that catalyze the dismutation of superoxide (O2-) radicals into molecular oxygen (O2) and H2O2 as a first line of defense against oxidative stress. Here, we show that glycine-functionalized copper(ii) hydroxide nanoparticles (Gly-Cu(OH)2 NPs) are functional SOD mimics, whereas bulk Cu(OH)2 is insoluble in water and catalytically inactive. In contrast, Gly-Cu(OH)2 NPs form water-dispersible mesocrystals with a SOD-like activity that is larger than that of their natural CuZn enzyme counterpart. Based on this finding, we devised an application where Gly-Cu(OH)2 NPs were incorporated into cigarette filters. Cigarette smoke contains high concentrations of toxic reactive oxygen species (ROS, >1016 molecules per puff) including superoxide and reactive nitrogen species which lead to the development of chronic and degenerative diseases via oxidative damage and subsequent cell death. Embedded in cigarette filters Gly-Cu(OH)2 NPs efficiently removed ROS from smoke, thereby protecting lung cancer cell lines from cytotoxic effects. Their stability, ease of production and versatility make them a powerful tool for a wide range of applications in environmental chemistry, biotechnology and medicine.
Subject(s)
Copper , Glycine , Hydroxides , Nanoparticles , Reactive Oxygen Species/isolation & purification , Superoxide Dismutase/chemistry , A549 Cells , Humans , Hydrogen Peroxide , Smoke , Tobacco ProductsABSTRACT
Surface functionalized ZrO2 nanoparticles show strong photoluminescence and are a versatile tool for cellular targeting due to their chemical functionality. They are highly photostable, biocompatible and amenable to coupling with bioligands (e.g. secondary goat anti-rabbit antibody (GAR) and tri-phenyl phosphine (TPP)) via carbodiimide chemistry. Antibody (GAR) functionalized ZrO2 nanoparticles were used to image the nuclear protein Sirt6, whereas triphenyl phosphonium ion (TPP) functionalized ZrO2 nanoparticles specifically targeted the mitochondria. The versatility and easiness of the ZrO2 surface modification opens up new possibilities for designing non-toxic water dispersible and photostable photoluminescent NPs.
ABSTRACT
Sulfite oxidase is a mitochondria-located molybdenum-containing enzyme catalyzing the oxidation of sulfite to sulfate in the amino acid and lipid metabolism. Therefore, it plays a major role in detoxification processes, where defects in the enzyme cause a severe infant disease leading to early death with no efficient or cost-effective therapy in sight. Here we report that molybdenum trioxide (MoO3) nanoparticles display an intrinsic biomimetic sulfite oxidase activity under physiological conditions, and, functionalized with a customized bifunctional ligand containing dopamine as anchor group and triphenylphosphonium ion as targeting agent, they selectively target the mitochondria while being highly dispersible in aqueous solutions. Chemically induced sulfite oxidase knockdown cells treated with MoO3 nanoparticles recovered their sulfite oxidase activity in vitro, which makes MoO3 nanoparticles a potential therapeutic for sulfite oxidase deficiency and opens new avenues for cost-effective therapies for gene-induced deficiencies.
Subject(s)
Metal Nanoparticles/chemistry , Molybdenum/chemistry , Nanotechnology/methods , Oxides/chemistry , Sulfite Oxidase/chemistry , Amino Acid Metabolism, Inborn Errors , Binding Sites , Electrochemistry , Electrodes , Electronics , Kinetics , Light , Nanoparticles/chemistry , Nanowires/chemistry , Oxidation-Reduction , Photochemistry , Photons , Sulfite Oxidase/deficiency , Zinc Oxide/chemistryABSTRACT
Polypeptides are successfully incorporated into poly(l-lactide) (PLLA) chains in a ring-opening polymerization (ROP) of l-lactide by using them as initiators. The resulting ABA triblock copolymers possess molecular weights up to 11000 g·mol(-1) and polydispersities as low as 1.13, indicating the living character of the polymerization process. In a nonaqueous emulsion, peptide-initiated polymerization of l-lactide leads to well-defined nanoparticles, consisting of PLLA-block-peptide-block-PLLA copolymer. These nanoparticles are easily loaded by dye-encapsulation and transferred into aqueous media without aggregation (average diameter of 100 nm) or significant dye leakage. Finally, internalization of PLLA-block-peptide-block-PLLA nanoparticles by HeLa cells is demonstrated by a combination of coherent anti-Stokes Raman spectroscopy (CARS) and fluorescence microscopy. This demonstrates the promise of their utilization as cargo delivery vehicles.
