ABSTRACT
A new sensitive, rapid and accurate spectrofluorimetric method, suitable for the determination of micromolar concentrations of iron(III) in bovine liver, using 4-hydroxyquinoline (4-HQ) in an alkaline medium (KOH 2.0 x 10(-2) mol/l) as fluorescent agent, is described. The fluorescence intensity of the working solutions was measured at lambda ex 305 nm and lambda em 380 nm. The observed decrease of the above mentioned intensity was mainly due to the quenching, caused by the interaction between the iron(III) to be analysed and the potentially fluorescent 4-HQ. The accuracy and the precision of the proposed method, after an experimental investigation, could be considered as very satisfactory. Potassium fluoride and triethylenetetramine solutions were successfully used for the masking of those metal cations existing in the bovine liver which interfere seriously with the determination of iron(III).
Subject(s)
Fluorescent Dyes/chemistry , Hydroxyquinolines/chemistry , Iron/analysis , Liver/chemistry , Spectrometry, Fluorescence/methods , Animals , Artifacts , Cattle , Hydrogen-Ion Concentration , Indicators and Reagents , Reference Standards , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
A new high-sensitive and at the same time accurate and well documented indirect spectrofluorimetric method for the determination of sub-micromolar concentrations of manganese(II) being as a normal trace element in the bovine liver, was described. The purpose of the experimental work is performed by means of two fluorescence intensity measurements; the measurement of the fluorescence (F1) of a 2.0 x 10(-5) mol/l solution of pure 2-hydroxyquinoline (2-HQ) (lambda ex: 320 nm: lambda em: 375 nm), and that of the fluorescence of the above solution after the addition of Mn(II) to be analyzed (F2). The observed difference of the fluorescence intensities measured (F1-F2) = delta F, is exclusively owed to the quenching of the Mn(II) on the free 2-HQ. The presence of various metal ions considerably interferes with the above described determination of Mn(II). For this reason, the separation and/or masking of these undesirable metal ions it is advisable to apply before any analytical praxis.
