ABSTRACT
The action of reducing, oxidizing and thiol-alkylating agents on early steps of Junin virus (JV) multiplication in Vero cells was investigated. The presence of reducing agents during virus adsorption as well as incubation of viral particles with these compounds before infection enhanced JV infectivity. On the contrary, the thiol-alkylating agent 5,5' dithiobis (2-nitrobenzoic acid) and the oxidizing compound potassium periodate showed an inhibitory effect, suggesting that sulfhydryl groups, and certain sugar moieties of viral glycoproteins play an important role in the first steps of JV infection. Also enzymatic treatment of cell monolayers and addition of concanavalin A to cultures prior to infection suggest that cellular glycoproteins are involved in virus attachment.
Subject(s)
Alkylating Agents/pharmacology , Junin virus/drug effects , Junin virus/physiology , Oxidants/pharmacology , Reducing Agents/pharmacology , Virus Replication/drug effects , Animals , Chlorocebus aethiops , Concanavalin A/pharmacology , Dithionitrobenzoic Acid/pharmacology , Dithiothreitol/pharmacology , Ficain/pharmacology , Membrane Glycoproteins/physiology , Mercaptoethanol/pharmacology , Periodic Acid/pharmacology , Potassium Compounds/pharmacology , Receptors, Virus/drug effects , Receptors, Virus/physiology , Sulfhydryl Reagents/pharmacology , Type C Phospholipases/pharmacology , Vero Cells , Virulence/drug effects , Virulence/physiologyABSTRACT
Se investigó la naturaleza bioquímica de las estructuras presentes en la superficie de células Vero, implicadas en la interacción con el virus Junín (cepa XJ C13) y una mutante de rango de huésped denominada Cl 67. Los tratamientos enzimáticos realizados a las células antes de la infección indican que las primeras moléculas que interaccionan con el virus Junín serían proteínas o grupos proteícos con aminoácidos básicos o aromáticos expuestos, existiendo diferencias en la afinidad hacia las cepas estudiadas (AU)
Subject(s)
Junin virus , Vero Cells/enzymology , ArgentinaABSTRACT
Se investigó la naturaleza bioquímica de las estructuras presentes en la superficie de células Vero, implicadas en la interacción con el virus Junín (cepa XJ C13) y una mutante de rango de huésped denominada Cl 67. Los tratamientos enzimáticos realizados a las células antes de la infección indican que las primeras moléculas que interaccionan con el virus Junín serían proteínas o grupos proteícos con aminoácidos básicos o aromáticos expuestos, existiendo diferencias en la afinidad hacia las cepas estudiadas
Subject(s)
Vero Cells/enzymology , Junin virus , ArgentinaABSTRACT
The chemical nature of cellular structures involved in the attachment of Junin virus (wild type XJC13 and host range mutant Cl 67) to Vero cells was investigated. Enzyme treatment of cells before virus infection indicated that whereas lipids are not directly involved in virus attachment, cellular proteins play a significant role in early interaction with JV. Moreover aromatic residues, leucine and basic amino acid seem to actively participate in this interaction with different affinity for the assayed strains.
Subject(s)
Enzymes/pharmacology , Junin virus/drug effects , Vero Cells/drug effects , Animals , Cells, Cultured , Chlorocebus aethiops , Female , Junin virus/physiology , Male , Vero Cells/physiology , Vero Cells/virologyABSTRACT
The chemical nature of cellular structures involved in the attachment of Junin virus (wild type XJC13 and host range mutant Cl 67) to Vero cells was investigated. Enzyme treatment of cells before virus infection indicated that whereas lipids are not directly involved in virus attachment, cellular proteins play a significant role in early interaction with JV. Moreover aromatic residues, leucine and basic amino acid seem to actively participate in this interaction with different affinity for the assayed strains.
