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1.
Neurotoxicol Teratol ; 30(6): 503-9, 2008.
Article in English | MEDLINE | ID: mdl-18565728

ABSTRACT

Amyloid precursor protein (APP) is overexpressed in the developing brain and portions of its extracellular domain, especially amino acid residues 96-110, play an important role in neurite outgrowth and neural cell differentiation. In the current study, we evaluated the developmental abnormalities caused by administration of exogenous APP(96-110) in sea urchin embryos and larvae, which, like the developing mammalian brain, utilize acetylcholine and other neurotransmitters as morphogens; effects were compared to those of beta-amyloid 1-42 (Abeta42), the neurotoxic APP fragment contained within neurodegenerative plaques in Alzheimer's Disease. Although both peptides elicited dysmorphogenesis, Abeta42 was far more potent; in addition, whereas Abeta42 produced abnormalities at developmental stages ranging from early cleavage divisions to the late pluteus, APP(96-110) effects were restricted to the intermediate, mid-blastula stage. For both agents, anomalies were prevented or reduced by addition of lipid-permeable analogs of acetylcholine, serotonin or cannabinoids; physostigmine, a carbamate-derived cholinesterase inhibitor, was also effective. In contrast, agents that act on NMDA receptors (memantine) or alpha-adrenergic receptors (nicergoline), and that are therapeutic in Alzheimer's Disease, were themselves embryotoxic, as was tacrine, a cholinesterase inhibitor from a different chemical class than physostigmine. Protection was also provided by agents acting downstream from receptor-mediated events: increasing cyclic AMP with caffeine or isobutylmethylxanthine, or administering the antioxidant, a-tocopherol, were all partially effective. Our findings reinforce a role for APP in development and point to specific interactions with neurotransmitter systems that act as morphogens in developing sea urchins as well as in the mammalian brain.


Subject(s)
Acetylcholine/analogs & derivatives , Amyloid beta-Peptides/pharmacology , Amyloid beta-Protein Precursor/pharmacology , Cannabinoids/metabolism , Embryonic Development/drug effects , Peptide Fragments/pharmacology , Sea Urchins/drug effects , Serotonin/analogs & derivatives , Acetylcholine/metabolism , Animals , Cannabinoids/agonists , Cannabinoids/pharmacology , Chlorpyrifos/pharmacology , Dose-Response Relationship, Drug , Drug Interactions , Embryo, Nonmammalian , Larva/drug effects , Sea Urchins/growth & development , Serotonin/metabolism , Serotonin/pharmacology , Time Factors
2.
Brain Res Bull ; 75(1): 94-100, 2008 Jan 31.
Article in English | MEDLINE | ID: mdl-18158101

ABSTRACT

Accumulation of beta-amyloid protein is an Alzheimer's disease hallmark but also may be mechanistically involved in neurodegeneration. One of its cleavage peptides, Abeta42, has been used to evaluate the mechanisms underlying amyloid-induced cytotoxicity and targeting of acetylcholine systems. We studied Sphaerechinus granularis sea urchin embryos which utilize acetylcholine and other neurotransmitters as morphogens. At a threshold concentration of 0.1 microM Abeta42, there was damage to the larval skeleton, accumulation of ectodermal cells in the blastocoele and underdevelopment of larval arms. Raising the Abeta42 concentration to 0.2-0.4 microM produced anomalies depending on the stage at which Abeta42 was introduced: at the first cleavage divisions, abnormalities appeared within 1-2 cell cycles; at the mid-blastula stage, the peak period of sensitivity to Abeta42, gastrulation was blocked; at later stages, there was progressive damage to the larval skeleton, digestive tract and larval spicules, as well as regression of larval arms. Each of these anomalies could be offset by the addition of lipid-permeable analogs of acetylcholine (arachidonoyl dimethylaminoethanol), serotonin (arachidonoyl serotonin) and cannabinoids (arachidonoyl vanillylamine), with the greatest activity exhibited by the acetylcholine analog. These results indicate that sea urchin embryos provide a model suitable to characterize the mechanisms underlying the cytotoxicity of Abeta42, as well as providing a system that enables the rapid screening of potential therapeutic interventions. The protection provided by neurotransmitter analogs, especially that for acetylcholine, points to unsuspected advantages of existing therapies that enhance cholinergic function, as well as indicating novel approaches that may prove protective in Alzheimer's disease.


Subject(s)
Amyloid beta-Peptides/toxicity , Disease Models, Animal , Drug Evaluation/methods , Embryonic Development/drug effects , Neurotoxicity Syndromes , Neurotransmitter Agents/therapeutic use , Peptide Fragments/toxicity , Age Factors , Animals , Dose-Response Relationship, Drug , Embryo, Nonmammalian , Neurotoxicity Syndromes/drug therapy , Neurotoxicity Syndromes/etiology , Neurotoxicity Syndromes/physiopathology , Sea Urchins/embryology
3.
Brain Res Bull ; 74(4): 221-31, 2007 Sep 28.
Article in English | MEDLINE | ID: mdl-17720543

ABSTRACT

Lower organisms show promise for the screening of neurotoxicants that might target mammalian brain development. Sea urchins use neurotransmitters as embryonic growth regulatory signals, so that adverse effects on neural substrates for mammalian brain development can be studied in this simple organism. We compared the effects of the organophosphate insecticide, chlorpyrifos in sea urchin embryos with those of the monoamine depleter, reserpine, so as to investigate multiple neurotransmitter mechanisms involved in developmental toxicity and to evaluate different therapeutic interventions corresponding to each neurotransmitter system. Whereas reserpine interfered with all stages of embryonic development, the effects of chlorpyrifos did not emerge until the mid-blastula stage. After that point, the effects of the two agents were similar. Treatment with membrane permeable analogs of the monoamine neurotransmitters, serotonin and dopamine, prevented the adverse effects of either chlorpyrifos or reserpine, despite the fact that chlorpyrifos works simultaneously through actions on acetylcholine, monoamines and other neurotransmitter pathways. This suggests that different neurotransmitters, converging on the same downstream signaling events, could work together or in parallel to offset the developmental disruption caused by exposure to disparate agents. We tested this hypothesis by evaluating membrane permeable analogs of acetylcholine and cannabinoids, both of which proved effective against chlorpyrifos- or reserpine-induced teratogenesis. Invertebrate test systems can provide both a screening procedure for mammalian neuroteratogenesis and may uncover novel mechanisms underlying developmental vulnerability as well as possible therapeutic approaches to prevent teratogenesis.


Subject(s)
Adrenergic Uptake Inhibitors/toxicity , Chlorpyrifos/toxicity , Embryonic Development/drug effects , Insecticides/toxicity , Neurotoxicity Syndromes , Reserpine/toxicity , Animals , Disease Models, Animal , Dose-Response Relationship, Drug , Drug Interactions , Embryo, Nonmammalian/drug effects , Neurotoxicity Syndromes/drug therapy , Neurotoxicity Syndromes/etiology , Neurotoxicity Syndromes/metabolism , Neurotoxicity Syndromes/physiopathology , Neurotransmitter Agents/therapeutic use , Sea Urchins/embryology
4.
Brain Res ; 925(2): 169-75, 2002 Jan 25.
Article in English | MEDLINE | ID: mdl-11792365

ABSTRACT

The uptake of principal salvageable nucleobase hypoxanthine was investigated across the basolateral membrane of the sheep choroid plexus (CP) perfused in situ. The results suggest that hypoxanthine uptake was Na+-independent, which means that transport system on the basolateral membrane can mediate the transport in both directions. Although the unlabelled nucleosides adenosine and inosine markedly reduce the transport it seems that this inhibition was due to nucleoside degradation into nucleobases in the cells, since non-metabolised nucleoside analogue NBTI did not inhibit the transport. The presence of adenine also inhibits hypoxanthine uptake while the addition of the pyrimidines does not show any effect, so it seems that the transport of purine nucleobases through basolateral membrane is mediated via a common transporter which is different from the nucleoside transporters. The inclusion of allopurinol in the perfusion fluid did not change the value and general shape of the curve for the uptake which suggest that degradation of hypoxanthine into xanthine and uric acid does not occur in the CP. The capacity of the CP basolateral membrane to transport hypoxanthine is high (90.63+/-3.79 nM/min/g) and close to the values obtained for some essential amino acids by the CP and blood-brain barrier, while the free diffusion is negligible. The derived value of Km (20.72+/-2.42 microM) is higher than the concentration of hypoxanthine in the sheep plasma (15.61+/-2.28 microM) but less than a half of the concentration in the CSF, which indicates that the transport system at basolateral membrane mostly mediates the efflux of hypoxanthine from the cerebrospinal fluid in vivo.


Subject(s)
Choroid Plexus/metabolism , Hypoxanthine/metabolism , Hypoxanthine/pharmacokinetics , Thioinosine/analogs & derivatives , Adenine/metabolism , Allopurinol/pharmacology , Animals , Binding, Competitive/drug effects , Biological Transport/drug effects , Biological Transport/physiology , Carbon Radioisotopes , Choroid Plexus/drug effects , Dose-Response Relationship, Drug , Hypoxanthine/cerebrospinal fluid , In Vitro Techniques , Mannitol/pharmacokinetics , Perfusion , Sheep , Sodium/metabolism , Thioinosine/pharmacology , Thymine/metabolism , Tritium , Uracil/metabolism , Xanthine Oxidase/antagonists & inhibitors
5.
J Drug Target ; 10(8): 633-6, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12683668

ABSTRACT

The aim of this study was to analyse the uptake of the synthetic nucleoside tiazofurin and glucoso-linker-tiazofurin conjugate (GLTC) into rat C6 glioma cells in vitro. Results indicated that C6 cells accumulated [3H] tiazofurin slowly with time and that accumulation was reduced by the presence of unlabelled GLTC in the medium which implies that GLTC competes with tiazofurin for transport sites. Uptake of [14C] 2 deoxy-glucose into these cells was very rapid and was not affected by the presence of unlabelled GLTC. To prove the true rate of uptake, the HPLC analysis of cellular extract was performed. After the 360 min of incubation in medium that contained 0.15 mM of tiazofurin, the sum of the concentration of tiazofurin and it's metabolite thiazole-adenine dinucleotide (TAD) in the cells was a total of approximately 4.8% of the amount added to each flask. After the same period of incubation in medium which contained 0.15 mM of GLTC, the sum of concentrations of conjugate, free tiazofurin and TAD represented less than 1/3 of the total concentration measured after the incubation with free tiazofurin and was further reduced in the presence of dipyridamole. Therefore, it can be concluded that GLTC shows some affinity for the nucleoside transporter, but the actual rate of uptake is low.


Subject(s)
Antimetabolites, Antineoplastic/metabolism , Glioma/metabolism , Glucose/chemistry , Ribavirin/metabolism , Animals , Antimetabolites, Antineoplastic/chemistry , Binding, Competitive/drug effects , Cell Membrane/metabolism , Chromatography, High Pressure Liquid , Deoxyglucose/pharmacology , Dipyridamole/pharmacology , Kinetics , Rats , Ribavirin/analogs & derivatives , Ribavirin/chemistry , Tumor Cells, Cultured
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