[Influence of calcium ionophore A23187 on neurotransmitter release in rat brain synaptosomes]. / Vliianie kal'tsevogo ionofora A23187 na osvobozhdenie neiromediatorov iz sinaptosom mozga krys.

The effect of calcium ionophore A23187 on the release of nonmetabolizable glutamate analogues [3H]D-aspartate and the exocytosis registered by fluorescent dyes in synaptosomes was investigated. It was shown that A23187 is able to induce neurotransmitter release both in calcium-containing and calcium-free medium, the effect in the latter case being more pronounced. Calcium ionophore is able to induce exocytosis registered by acridine orange and FM 2-10. The influence of A23187 on the fluorescence of acridine orange was mainly calcium-independent, whereas the change in the fluorescence of FM 2-10 was calcium-dependent. It was suggested that the calcium-independent increase in acridine orange fluorescence is related to the dissipation of pH gradient in synaptic vesicles. Probably, the calcium-independent release of D-aspartate is also associated with the dissipation of pH gradient and subsequent leakage of neurotransmitters.

Effects of nitric oxide donors on Ca2+-dependent [14C]GABA release from brain synaptosomes: the role of SH-groups.

Nitric oxide (NO) modulates processes of synaptic transmission at pre- and postsynaptic levels. In the present work we studied the mechanisms of action of NO on [gamma-14C]amino-n-butyric acid ([14C]GABA) release in rat cortical synaptosomes. NO donors--S-nitroso-L-cysteine and hydroxylamine (but not sodium nitroprusside)--inhibited the neurotransmitter efflux in a concentration range from 10 microM to 1 mM. Nitrosocysteine completely and selectively suppressed the Ca2+-dependent (vesicular) [14C]GABA release, while not affecting the Ca2+-independent component of the [14C]GABA transport. The influence of NO donors was not related to activation of guanylyl cyclase, since the membrane-permeable cGMP analog dibutyryl-cGMP did not mimic and the guanylyl cyclase inhibitor methylene blue did not change the NO effects. In contrast, the membrane-permeable SH-reagent N-ethylmaleimide (NEM) resembled the effects of NO donors on the Ca2+-dependent [14C]GABA release. The degree of inhibition of the release by nitrosocysteine, hydroxylamine, and NEM correlated with their ability to oxidize intra-synaptosomal SH-groups. These data suggest that synaptosomal sulfhydryl groups are the target for NO action at the presynaptic level. The NO-induced oxidation of thiols may be involved in physiological and, especially, pathological effects of nitric oxide in the central nervous system.

Bioenergetic response of isolated nerve terminals of rat brain to osmotic swelling.

The swelling of nerve terminals of rat brain in a hypotonic medium (230 mOsm) induced the potential-independent entrance of 45Ca2+ into synaptosomes and intrasynaptosomal mitochondria that changed the energy status of synaptosomes, the rate of O2 consumption and the content of ATP being decreased. The ratio ATP/ADP decreased from 6.5 +/- 0.26 (310 mOsm medium) to 3.1 +/- 0.18 (the medium 230 mOsm). Studies on the equilibrium distribution of K+ (86Rb+) and [3H]TPP+ showed that contents of these cations in the nerve terminals were virtually the same on incubation in both iso- and hypotonic media. This indicated that the swelling did not damage intrasynaptosomal mitochondria and plasma membranes of the synaptosomes. The inhibition of oxidative phosphorylation increased twofold the rate of glycolysis. The incubation of synaptosomes in calcium-free medium (230 mOsm) in the presence of EGTA (1 mM) prevented the inhibition of oxidative phosphorylation and synthesis of ATP by the osmotic swelling. Ruthenium Red (10 microM) in the medium 230 mOsm inhibited the entrance of 45Ca2+ into the intrasynaptosomal mitochondria and normalized the oxidative phosphorylation to the control level (310 mOsm medium). The decrease in the energy potential of synaptosomes induced by the hypoosmotic shock is suggested to be associated with the increase in Ca2+ content in the cytoplasm, its transport into the mitochondria, and the inhibitory effect on oxidative phosphorylation.

[Effect of ischemic damage factors on lipid peroxidation in rat brain synaptosomes]. / Vliianie faktorov ishemicheskogo povrezhdeniia na perekisnoe okislenie lipidov v sinaptosomakh mozga krys.

Accumulation of TBA-reactive substances after a 40 min incubation of rat brain synaptosomes at 37 degrees C was analysed. A lowering of pH to 6.5-5.5 or arachidonic acid (0.1-1.0 mM) increased lipid peroxidation which was blocked by antioxidants. Acidosis (pH 6.0) and arachidonic acid used in combination had a strong synergic effect. Depolarisation of plasma membranes or intrasynaptosomal mitochondria were without influence on lipid peroxidation at neutral or acid pH. The results support a leading role of acidosis and phospholipases in stimulation of peroxidation under ischaemia.

Osmotic regulation of sodium pump in rat brain synaptosomes: the role of cytoplasmic sodium.

The effect of hypoosmolality of incubation medium on the rat of ouabain-sensitive 86Rb+ transport in rat brain synaptosomes was studied. A decreased osmolality from 310 to 250 mOsm increased the rate of 86Rb+ uptake from 3.72 to 6.23 nmol/mg of protein min. To evaluate the involvement of cytoplasmic sodium in sodium pump stimulation inhibitors of ion channels and transport pathways able to increase [Na+]in were used. Tetrodotoxin (1 microM), amiloride (0.5 mM) and verapamil (0.1 mM) had no influence on the osmotic response of the sodium pump. The decrease of sodium concentration in incubation medium to 15 mM, leading to a practical loss of its transmembrane gradient, did not abolish stimulation of pump. No increase in 22Na+ influx or intrasynaptosomal sodium content was registered at hypotonic conditions. It is suggested that osmotic regulation of Na+,K(+)-ATPase is not connected with an increase of internal sodium through opening of sodium channels, or with activation of other membrane sodium-transporting systems.

[Osmotic regulation of furosemide-sensitive K+ (86Rb+) transport in rat brain synaptosomes]. / Osmoticheskaia reguliatsiia furosemid-chuvstvitel'nogo transporta K+ (86Rb+) v sinaptosomakh mozga krys.

In the rat brain synaptosomes the furosemide-sensitive component of 86Rb+ uptake constituted 30.8% of the total uptake in the medium containing 132 mM Na+. A decrease in the medium tonicity from 310 to 230 mOsm increased the rate of 86Rb+ uptake from 2.38 +/- 0.58 to 7.12 +/- 0.52 nMoles/mg of protein/min.

[Osmotic regulation of the sodium pump in rat brain synaptosomes]. / Osmoticheskaia reguliatsiia natrievogo nasosa v sinaptosomakh golovnogomozga krys.

Effect of medium osmolarity on 3H-ouabain binding and the rate of ouabain-sensitive 86Rb+ transport in the rat brain synaptosomes was studied. A decrease in tonicity to 230 mOsm increases both parameters indicating the activation of the sodium pump upon synaptosome swelling. The effect is retained in the absence of inside-oriented Na+ gradient, i. e. a rise in Na(in) is not responsible for hypoosmotic activation. Colchicine (5mM) decreased and cytochalasin B (40 microM) increased the ouabain binding. In the presence of cytochalasin B the inhibition of binding observed under hypotonic conditions was shifted to higher osmolarity values. It is suggested that volume regulation of the sodium pump is controlled by the cytoskeleton elements.

[Cytoskeletal regulation of the sensitivity of brain synaptosomes to the depolarizing action of veratrine]. / Reguliatsiia tsitoskeletom chuvstvitel'nosti sinaptosom mozga k depoliarizuiushchemu deistviiu veratrina.

Using a radioactive permeant cation 3H-tetraphenylphosphonium, the sensitivity of rat brain synaptosomes to depolarizing action of veratrine, which specifically opens the sodium channels, was compared before and after destruction of microtubules and microfilaments. Depolymerization of microtubules with colchicin and vinblastine decreased an apparent affinity of veratrine to its receptor in the channel, while destruction of microfilaments with cytochalasin B had the opposite effect. Colchicine did not change allosteric interactions between the receptor for veratrine and that for scorpion venom in the sodium channel evaluated by the ability of scorpion venom to facilitate veratrine-induced depolarization of synaptosomes. It is suggested that two main cytoskeleton subsystems control the state of sodium channels in the nerve ending.

[Calcium transport in brain synaptosomes during depolarization. The role of potential-dependent channels and Na+/Ca2+ metabolism]. / Transport kal'tsiia v sinaptosomakh mozga pri depoliarizatsii. Rol' potentsialzavisimykh kanalov i Na+/Ca2+-obmena.

The contribution of Ca2+ channels and Na+/Ca2+ exchange to Ca2+ uptake in rat brain synaptosomes upon long- (t greater than or equal to 30 s) and short-term (t less than 30 s) depolarization by high K+ was studied by measuring the 45Ca content and free Ca2+ concentration (from Quin-2 fluorescence). At 37 degrees C, the system responsible for the K+-stimulated uptake of 45Ca (t greater than or equal to 30 s) and the Na+/Ca+ exchanger are characterized by a similar concentration dependence of external Ca2+ (Ca0(2+] and K0+ as well as by an equal sensitivity to verapamil (Ki = approximately 20-40 microM) and La2+ (Ki = approximately 50 microM). These data and the results from predepolarization suggest that the 45Ca entry into synaptosomes at t greater than or equal to 30 s is due to the activation of Na+/Ca+ exchange caused by its electrogenic component, while the insignificant contribution of Ca2+ channels can be accounted for by their inactivation. At low temperatures (2-4 degrees C) which decelerate the inactivation, the initial phase of 45Ca uptake is fully provided for by Ca2+ channels, showing a lower (as compared to the exchanger) affinity for Ca0(2+) (K0.5 greater than 1 mM)m a greater sensitivity to La3+ (Ki = approximately 0.2-0.3 microM) and verapamil (Ki = approximately 2-3 microM); these channels are fully inactivated by predepolarization with K0+, ouabain and batrachotoxin. The Ca2+ channels can be related to T-type channels, since they are not blocked by nicardipine and niphedipine.

[Effect of microenvironment on the conformation of m-cholinoreceptors in brain synaptic membranes]. / Vliianie mikrookruzheniia na konformatsionnoe sostoianie m-kholinoretseptorov sinapticheskikh membran mozga.

Sensitivity of m-cholinergic receptor of synaptic membranes to SH-reagents (PChMB and NEM) was compared before and after its solubilization with detergents. PChMB blocked specific binding of m-antagonist QNB to both forms of the receptor in equal extent, while NEM was effective only after solubilization. After the membranes modification with butanol or arachidonic acid the membrane-bound receptor became sensitive to NEM. The solubilized receptor lost its ability to allosteric inhibition by acid. It is suggested that the receptor conformation is under the control of the membrane microenvironment.

Inactivation of muscarinic acetylcholine receptors in brain synaptic membranes by free fatty acids. Evaluation of the role of lipid phase.

Arachidonic, linolenic and linoleic acids decreased the binding of the m-cholinergic antagonist [3H] QNB and did not affect the ratio of high to low affinity binding sites to the agonist carbamoylcholine in rat brain synaptic membranes. In the presence of arachidonic acid, SH-reagent N-ethylmaleimide acquired the ability to block QNB binding to receptor. Lipids in the bilayer and annular regions were probed by fluorescence of 1,6-diphenyl-1, 3, 5-hexatriene and pyrene. A microviscosity drop induced by increasing temperature from 10 to 37 degrees C did not affect the level of QNB equilibrium binding, whereas arachidonic acid strongly inhibited the binding at concentrations inducing the same drop in microviscosity as that induced by heating. For various unsaturated fatty acids an equal extent of receptor blocking was reached at quite different degrees of bilayer fluidization, the state of annular lipid being not changed under these conditions. It is suggested that the effect of unsaturated acids is reached through their direct interaction with the receptor, which undergoes a conformational change, rather than by an alteration of the physical state of the lipid phase of the membrane.

[Membrane potential and transport of neuromediators in synaptosomes of the brain of rats with spontaneous hypertension]. / Membrannyi potentsial i transport neiromediatorov v sinaptosomakh golovnogo mozga krys so spontannoi gipertenziei.

The transport of 3H-GABA, 14C-choline, 14C-noradrenaline and 3H-serotonin in brain synaptosomes of spontaneously-hypertensive rats (SHR) which is controlled by the carrier whose activity is dependent on the sodium gradient and electric potential of the plasma membrane was studied. The initial rate of GABA uptake by SHR synaptosomes was reduced by 25%. The steady state of the content of GABA, noradrenaline and serotonin in SHR synaptosomes was reduced by 15-20%, whereas the steady state of choline content by 35%. The differences are levelled when synaptosomes are depolarized, both due to the elimination of K+-diffusion potential, and the opening of Na channels. An increase in the extracellular calcium concentration up to 1 mmol results in a 20% synaptosomal GABA rise that is unrelated to membrane hyperpolarization. In the presence of the Ca-ionophore A-23187, a similar effect is observed with a 1-2 mmol Ca2+ concentration. The electric potential of SHR synaptosomes which is studied by means of the fluorescent dye dis-C3-(5) was shown to be reduced by 5-10 mV. Partial depolarization of SHR synaptosomes is supposed to be the principal cause of the decrease in both the initial rate of uptake and the steady state of the content of neuromediators.

Effect of tetracaine on veratrine-mediated influx of sodium into rat brain synaptosomes.

Depolarization of synaptosomes with veratrine (0.1 mg/ml) or 50 mmol/l of KCl results in the release of radioactivity from 14C-choline loaded synaptosomes with partial dependence on external Ca2+. Like tetrodotoxin, tetracaine prevented veratrine but not KCl action, with a half-maximal effect at approximately 10(-5) mol/l of anesthetic. A similar half-maximal value was obtained for tetracaine blockade of veratrine-stimulated tetrodotoxin-sensitive 22Na influx into synaptosomes, complete blockage being achieved at 10(-4) mol/l. At this concentration tetracaine failed to modify Ca2+ channels measured by KCl-induced 45Ca uptake. Microviscosity of a lipid bilayer in synaptic membranes evaluated with 5- and 16-doxylstearate spin labels decreased at tetracaine concentrations exceeding 10(-3) mol/l. It is suggested that Na+ channels of synaptosomes are blocked by direct action of anesthetic or through changes in the channel annular lipids.

[Do anesthetics cause conformational changes in the majority of proteins in synaptic membranes?]. / Vyzyvaiut li anestetiki konformatsionnye perestroiki bol'shinstva belkov v sinapticheskikh membranakh?

Cationic and neutral anesthetics at concentrations 1--10 mM increase SH-group accessibility to DTNB in the rat brain synaptic membranes, these effects for NEM-reactive groups being 5--10 times lower. High ionic strength depresses partially the influence of cationic but not neutral anesthetics in experiments with DTNB. Pronase treatment causing cleavage of all membrane proteins effects the anesthetic action in a small extent only. It is suggested that changes in the number of exposed SH-groups in the presence of anesthetics are related to modification of lipid phase rather than to conformational transitions in a majority of membrane proteins.

[Effect of physiologic concentrations of local anesthetics of the procaine series on the structural and functional state of cerebral synaptosome membranes]. / Vliianie fiziologicheskikh kontsentratsii lokal'nykh anestetikov prokainovogo riada na strukturnoe i funktsional'noe sostoianie sinaptosomal'nykh membran mozga.

Studies are presented of the effect of procaine group anesthetics on rat brain synaptosome stability to dodecyl sulfate and on catalytic properties of the membrane bound alkaline phosphatase. The dose curves of detergent stability are characterized by two maxima, one at 3.10(-4) M for tetracaine, 2.10(-6) M for lidocaine and 5.10(-5) M for procaine; the other being at 10(-3) M for all anesthetics. The curves of Vmax and KM versus procaine concentration to exhibit the minimum at 5.10(-7) M and maximum at 3,2.10(-6) M. Procaine at 5.10(-7) M increases enthalpy and enthropy of membraneous alkaline phosphatase. It is suggested that interactions between anesthetics and centers of high affinity lead to synaptosome structural rearrangements, which affect the properties of membraneous enzymes.

[Thermal structural transitions in the membranes of fat cells]. / Termicheskie strukturnye perestroiki v membranakh zhirovykh kletok.

Studies have been carried out of the structural state of plasma membranes of epididimal fat cells from rats at 5--40 degrees C. The temperature dependence of the fluorescence intensity bound ANS and the rate of solubilization by 0.2% sodium dodecyl sulfate have shown sharp changes at 19--24 degrees C. At the same temperature intervals there was a break in the Arrhenius curve for activity of the membrane 5'-nucleotidase. ANS fluorescence and light scattering of liposomes from membrane lipids have revealed discontinuities at 21 degrees and 29--30 degrees C. The data are interpreted as thermal rearrangements initiated in the lipid membrane phase.

[Structural rearrangements in synaptosomal membranes of the brain]. / Strukturnye perestroiki v sinaptosomal'nykh membranakh mozga

Studies were carried out on structural state of synaptosomes and synaptic plasma membranes from brain of rats and guinea pigs at 5--50C. In membranes from guinea pig brain there were two breaks at 8 and at 18--20C in the curves of temperature dependence of light scattering. At the same temperatures adhesive properties of membranes had changed in jump-like manner when evaluating the degree of aggregation at pH4. The degree of aggregation in membranes from rat brain as well as rate of solubilization by 0.2% SDS (stoped-flow measurements) are characterized by single break point at 27C in Arrhenius curves. The breaks were interpreted as phase transitions with membrane surface involvement. Chlorpromazine and tetraciane (10(4)--10(3) M) abolished or modified these transitions. Because the number of negative charges and tryptophanyl and ANS fluorescence parameters were unchanged it was suggested that redistribution of ionogenic and hydrophobic goups took place at membrane surface during phase transitions.