ABSTRACT
30 strains of Plesiomonas shigelloides isolated from patients with acute diarrheal disease at different health centers of the country were studied. The were phenotypically characterized by conventional biochemical tests and the antimicrobial susceptibility to 11 drugs was determined by the Kirby Bauer's method. It was found that the strains of Plesiomonas shigelloides were sensitive to 7 and resistant to 6 of the investigated drugs. The presence of plasmids in 12 of the 29 analyzed strains was determined and the diversity in their plasmidic patterns was proved.
Subject(s)
Anti-Bacterial Agents/pharmacology , Plasmids/isolation & purification , Plesiomonas/drug effects , Plesiomonas/genetics , Acute Disease , DNA, Bacterial/isolation & purification , Diarrhea/microbiology , Feces/microbiology , Humans , Microbial Sensitivity Tests , PhenotypeABSTRACT
By means of the polymerase chain reaction (PCR) it was obtained a probe for the gen that codifies the subunit B of cholerae toxin (CTxB), which carried a Vibrio cholerae 01 reference strain. The checking of the amplified product was performed by using the hybridization techniques in colonies. This product hybridized with the gen that codifies for the subunit B of cholerae toxin isolated from Peru and Ecuador, representing the present epidemics in Latin America, but it did not so with the phylogenetically related strains.
Subject(s)
Cholera Toxin/genetics , In Situ Hybridization/methods , Vibrio cholerae/genetics , Bacteriological Techniques , Ecuador , Genes, Bacterial , Humans , India , Peru , Polymerase Chain Reaction , Vibrio cholerae/isolation & purificationABSTRACT
Twenty-seven cases of Aeromonas isolated from 300 five-years children with acute diarrheal disease (EDA) were studied, with the aim for demonstrating the occurrence of some phenotypical markers associated with enteropathogenicity; among them were lysine decarboxylation, acetil methyl carbinol production, enterotoxigenicity, cytotoxicity and hemolysis. The percentage of analysed strains had two or more of the investigated markers, and 13 (48.1%) lysed the rabbit erythrocytes with titres higher than than 1:16. The presence of markers in Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae was demonstrated.
Subject(s)
Aeromonas/genetics , Diarrhea, Infantile/microbiology , Diarrhea/microbiology , Gram-Negative Bacterial Infections/microbiology , Acute Disease , Aeromonas/isolation & purification , Aeromonas/pathogenicity , Child, Preschool , Cuba , Humans , Infant , PhenotypeABSTRACT
100 Aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from January to July, 1990. Using the Janda-modified Popoff's and Veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in Aeromonas sobria, Aeromonas hydrophila, and Aeromonas caviae, using also supplementary tests.
Subject(s)
Aeromonas/classification , Acute Disease , Aeromonas/isolation & purification , Bacteriological Techniques , Child, Preschool , Cuba , Diarrhea/microbiology , Diarrhea, Infantile/microbiology , Feces/microbiology , Humans , InfantABSTRACT
A study was conducted on 40 Mycobacterium fortuitum strains isolated from symptomatic patients suffering from respiratory diseases and skin lesions. Their susceptibility to different antimicrobial agents was determined by the broth microdilution method, measuring minimal inhibitory concentration. There was sensitivity of the strains to gentamicin and tetracycline as well as resistance to the tuberculostatic drugs used (isoniazid and ethambutol) and cefalotin.
Subject(s)
Mycobacterium fortuitum/drug effects , Anti-Bacterial Agents/pharmacology , Antitubercular Agents/pharmacology , Bacteriological Techniques , Ethambutol/pharmacology , Gentamicins/pharmacology , Humans , Microbial Sensitivity Tests , Mycobacterium fortuitum/growth & development , Tetracycline/pharmacologyABSTRACT
The presence of Yersinis enterocolitica was investigated in feces of 1,300 children younger than 5 years, hospitalized because of presenting acute diarrheic disease in Cuba during 1985-1988 period at different provincial pediatric hospital, as well as in Havana City. Selective agar base for Yersinia was used supplemented with cephsuloidin, Irgasan and novoviocin (CIN) and buffered saline solution as enrichment medium. This last one at 4 degrees C during 21 days with weekly resowings to the selective medium. Isolation of Yersinia enterocolitica was not obtained in any of the patients under study.