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1.
Appl Environ Microbiol ; 79(15): 4744-50, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23793628

ABSTRACT

Lymph nodes (mandibular, mesenteric, mediastinal, and subiliac; n = 68) and fecal (n = 68) and hide (n = 35) samples were collected from beef carcasses harvested in an abattoir in Mexico. Samples were analyzed for Salmonella, and presumptive colonies were subjected to latex agglutination. Of the isolates recovered, a subset of 91 was characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility phenotyping. Salmonella was isolated from 100% (hide), 94.1% (feces), 91.2% (mesenteric), 76.5% (subiliac), 55.9% (mandibular), and 7.4% (mediastinal) of samples. From the 87 typeable isolates, eight Salmonella enterica serotypes, including Kentucky (32.2%), Anatum (29.9%), Reading (17.2%), Meleagridis (12.6%), Cerro (4.6%), Muenster (1.1%), Give (1.1%), and Mbandaka (1.1%), were identified. S. Meleagridis was more likely (P = 0.03) to be recovered from lymph nodes than from feces or hides, whereas S. Kentucky was more likely (P = 0.02) to be recovered from feces and hides than from lymph nodes. The majority (59.3%) of the Salmonella isolates were pansusceptible; however, multidrug resistance was observed in 13.2% of isolates. Typing by PFGE revealed that Salmonella strains generally clustered by serotype, but some serotypes (Anatum, Kentucky, Meleagridis, and Reading) were comprised of multiple PFGE subtypes. Indistinguishable PFGE subtypes and, therefore, serotypes were isolated from multiple sample types, and multiple PFGE subtypes were commonly observed within an animal. Given the overrepresentation of some serotypes within lymph nodes, we hypothesize that certain Salmonella strains may be better at entering the bovine host than other Salmonella strains or that some may be better adapted for survival within lymph nodes. Our data provide insight into the ecology of Salmonella within cohorts of cattle and offer direction for intervention opportunities.


Subject(s)
Cattle Diseases/microbiology , Polymorphism, Genetic , Salmonella Infections, Animal/microbiology , Salmonella/classification , Salmonella/genetics , Abattoirs , Animals , Cattle , Cattle Diseases/epidemiology , Drug Resistance, Multiple, Bacterial , Electrophoresis, Gel, Pulsed-Field/veterinary , Feces/microbiology , Lymph Nodes/microbiology , Mexico , Microbial Sensitivity Tests/veterinary , Phylogeny , Salmonella/drug effects , Salmonella/isolation & purification , Salmonella Infections, Animal/epidemiology , Serotyping/veterinary , Skin/microbiology
2.
Rev. bioméd. (México) ; 10(2): 93-101, abr.-jun. 1999. tab
Article in Spanish | LILACS | ID: lil-254628

ABSTRACT

Introducción. La producción porcina ocupa un lugar importante dentro de la producción animal nacional y local. Los laboratorios de diagnóstico veterinario, representan una fuente de información para determinar el estado de salud de los animales en determinada región. El conocimiento de los problemas de salud de los porcinos en nuestro estado, representa uno de los aspectos básicos para el control y prevención de las enfermedades. El trabajo reporta los diagnósticos relevantes durante el periodo 1988-1997. Material y métodos. Se revisaron los reportes anuales de los diagnósticos realizados en el Departamento de Patología de Facultad de Medicina Veterinaria y Zootecnia de la Universidad Autónoma de Yucatan. Basándose en su frecuencia e importancia epidemiológica se seleccionaron 550 diagnósticos. Los diagnósticos se basaron en la observación de lesiones macroscópicas y/o histopatología y/o el aislamiento del agente causal. Resultados. Las afecciones más frecuentes fueron: en el sistema respiratorio; Actinobacillus pleuropneumoniae, Pasterurella multocida y lesiones sugestivas a Mycoplasma hyopnenumoniae. En sistema digestivo degeneración hepático y enteropatía proliferativa porcina. En sistema nervioso las meningoencefalitis no supurativos y meningitis supurativas por Streptococos a hemoliticos. Destacaron las lesiones sugestivas de Haemophilus parasuis. Conclusiones. Las enfermedades diagnosticadas y su frecuencia, coinciden con las reportadas como importantes en la porcicultura mundial. Los cambios en los sistemas de producción han modificado la presentación de los problemas de salud animal a través de los años


Subject(s)
Animals , Swine Diseases/diagnosis , Veterinary Public Health , Mexico
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