ABSTRACT
Porcine reproductive and respiratory syndrome virus (PRRSV) still poses a threat to the swine industry worldwide. Currently, commercial vaccines against PRRSV, which consist of modified live or inactivated virus, reduce symptoms and viremia in immunized pigs, but efficacy against heterologous strains is variable. This has led to the development of subunit vaccines that contain viral antigens that show the highest variability. In this work, a chimeric protein comprising short amino acid sequences from glycoprotein 3 (GP3), glycoprotein 4 (GP4), glycoprotein 5 (GP5), and M (matrix protein) proteins of PRRSV was designed and expressed in Escherichia coli. This protein, designated as PRRSVchim, was purified by immobilized metal affinity chromatography and evaluated. PRRSVchim was identified by immunoglobulin G (IgG) presence in serum samples from PRRSV-positive pigs. Also, the protein probed to be antigenic in immunized mice and piglets and provided some degree of protection against challenge with a PRRSV field isolate. These results show the potential of PRRSVchim protein for both PRRSV diagnostic and immunoprophylaxis.
Subject(s)
Antigens, Viral/immunology , Porcine Reproductive and Respiratory Syndrome/prevention & control , Porcine respiratory and reproductive syndrome virus/immunology , Recombinant Fusion Proteins/immunology , Viral Proteins/immunology , Viral Vaccines/immunology , Animals , Antibodies, Viral/blood , Antibodies, Viral/immunology , Antigens, Viral/administration & dosage , Antigens, Viral/genetics , Epitopes/genetics , Escherichia coli , Female , Glycoproteins/administration & dosage , Glycoproteins/genetics , Glycoproteins/immunology , Glycoproteins/isolation & purification , Immunogenicity, Vaccine , Immunoglobulin G/blood , Immunoglobulin G/immunology , Mice , Porcine Reproductive and Respiratory Syndrome/immunology , Porcine Reproductive and Respiratory Syndrome/virology , RAW 264.7 Cells , Recombinant Fusion Proteins/administration & dosage , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/isolation & purification , Swine , Vaccination/veterinary , Vaccines, Synthetic/administration & dosage , Vaccines, Synthetic/genetics , Vaccines, Synthetic/immunology , Vaccines, Synthetic/isolation & purification , Viral Proteins/administration & dosage , Viral Proteins/genetics , Viral Proteins/isolation & purification , Viral Vaccines/administration & dosage , Viral Vaccines/genetics , Viral Vaccines/isolation & purificationABSTRACT
Actibacillus pleuropneumonia (App) es el agente causal de la pleuroneumonía contagiosa porcina (PCP), este microorganismo puede ser clasificado en 12 serotipos o puede ser no tipificable. Se aislaron 81 cepas de A. Pleuropneumoniae de pulmones neumónicos de cerdo. Todas las cepas fueron serotipificadas por coagulación (CoA) y hemoaglutinación indirecta (HI), 43 cepas (53 por ciento) fueron clasificadas en el mismo serotipo por las dos técnicas, 31 correspondieron al serotipo 1, nueve al serotipo 5 y una de los serotipos 3, 4 y 6. Catorce cepas poliaglutinaron al usar CoA, pero fueron clasificadas en el serotipo 1 por la HI. El 13 por ciento de las cepas fueron clasificadas en diferente serotipo por cada una de las pruebas usadas. Finalmente, 13 cepas fueron no tipificables por la HI y clasificadas en diferentes serotipos por la CoA. Se sugiere el uso de la CoA para la tipificación rutinaria, mientras que la HI se recomienda para la identificación final de los aislamientos. En relación con los serotipos identificados, los resultados de esta investigación coinciden con lo registrado por otros autores, ya que de 81 cepas, 45 correspondieron al serotipo 1, y nueve al serotipo 5; lo que confirma que estos dos serotipos son los más frecuentemente aislados en México
