Plastic invasion tolling: First evaluation of microplastics in water and two crab species from the nature reserve lagoonary complex of Kune-Vain, Albania.

Microplastic (MP) pollution is an emerging global challenge and actually has become a reality in aquatic ecosystems in Albania. According to the World Wildlife Fund (WWF) report of 2019, Albania, is one of the most problematic countries, with the highest percentage of untreated plastic waste, 73 %, and one of the top four countries with the highest norm of untreated plastic waste in the Mediterranean. This study evaluates and characterizes for the first time the MPs in water, sediment, and gastrointestinal tracts of two crab species, C. aestuarii and C. sapidus, common inhabitants of the lagoonary complex of Kune-Vain Nature Reserve, known for their important role in the lagoon ecosystem. The results showed that all sampled crabs had MPs in their gut in an average of 11.0 ± 1.85 items g-1, while the total MPs content in water ranges from 370 to 750 MPs per L-1. No significant difference in the content of MPs between the two crab species was found and a positive Pearson correlation, between microplastic abundance in the water and in the crabs regardless of species, was confirmed. The composition of microplastics showed consistency in crabs, sediment, and water, with fibers and pellets as the dominant types followed by microbeads, and fragments. Characterized MPs varied in size from <0.1 mm to 0.1-0.5 mm, showing variable colors of black, blue, and red domination. Fourier Transform Infrared Spectroscopy (FT-IR) analysis of the chemical composition of microplastics distinguished presence of the high-density polyethylene (HDPE), polypropylene (PP), polyethylene (PE), and low-density polyethylene (LDPE), which showed consistency in water, sediment, and crab samples. In conclusion, high levels of MPs pollution observed in the Kune-Vain complex represent a serious threat to the lagoon ecosystem and to the local inhabitants. Furthermore, studies on MPs' impact on biota and local population health are urgently required.

Evaluation of neutrophil-to-lymphocyte ratio and immune response in patients vaccinated with Pfizer-Biontech vaccine.

INTRODUCTION: Research on SARS-CoV-2 virus has focused on aspects such as treatment, virology, epidemiology and vaccine development. The efficacy of vaccines against SARS-CoV-2 is important for controlling the pandemic. This study assessed how the immune response is affected by age and gender, and its role in causing inflammation as measured by neutrophil-to-lymphocyte ratio (NLR) in vaccinated patients versus non-vaccinated COVID-19 negative patients. METHODOLOGY: A case-control study was done involving 187 randomly selected patients who had undergone laboratory examinations to evaluate the SARS-CoV-2 IgG antibody titer and hematological parameters at 21 to 31 days after the second dose of vaccination. Patients were divided into case and control groups according to their vaccination status. RESULTS: The average age among the cases was 51 ± 13 years whereas the average age among the control group was 47 ± 15 years. In cases where the response to immunization was measured by SARS-CoV-2 IgG antibody, results had a median of 7.7 U/mL characterized by a large variation (p < 0.0001). There was no significant difference based on age (p = 0.451) and gender (p = 0.622) in SARS-CoV-2 IgG antibody titers in patients vaccinated with two doses of Pfizer-BioNTech COVID-19 vaccine. In addition, there was no significant difference in NLR ratio between cases and controls (p = 0.117). CONCLUSIONS: Our data showed that there is no inflammation at 21 to 31 days post vaccination with Pfizer-BioNTech COVID-19 vaccine, regardless of age and gender, based on the hematological parameters.

Screening for prostate cancer: a study on the free and total prostate specific antigen.

BACKGROUND: A variety of biomarkers have been developed to monitor growth of cancerous diseases and to detect them at an early stage. Prostate-specific antigen (PSA) is a valuable prostate cancer biomarker that is now widely used for population screening, diagnosis, and monitoring of patients with prostate cancer. Other factors than prostate cancer can cause elevation of PSA levels therefore, free prostate specific antigen measurements in serum have been proposed in order to improve the specificity of laboratory identification of prostate cancer. AIM: The aim of our study was to evaluate the diagnostic significance of both total PSA and Free PSA in discriminating prostate cancer from other prostate diseases. MATERIALS AND METHODS: Our study group consisted of 1201 males admitted at outpatient clinic aged between 35 and 84 years old (mean age 63 years). All laboratory measurements were performed on serum samples. The data were statistically analyzed by using descriptive statistics for Windows. RESULTS: The mean total PSA concentration evaluated among 1038 patients was 16.17 ng/mL whereas only Free PSA concentration was evaluated in 163 serum samples and resulted in a mean value of 2.67 ng/ml. In order to calculate the correlation between total and free PSA, data among 69 /1038 patients were further analyzed through statistical program software package for data analysis. CONCLUSIONS: Measuring serum free PSA concentrations along with PSA concentrations may provide higher accuracy for detecting prostate cancer and might eliminate unnecessary biopsies in the men with PSA of more than 4.0 ng/mL.

Hemorrhagic fever with renal syndrome in Albania. Focus on predictors of acute kidney injury in HFRS.

BACKGROUND: Hemorrhagic fever with renal syndrome (HFRS) is a rodent borne zoonosis, caused by the members of the family Bunyaviridae, genus Hantavirus. The main clinical features of the infection by this virus family are fever, thrombocytopenia and acute kidney injury. OBJECTIVE: The aim of our study was to identify, for the first time, characteristic features of HFRS in the Albanian population. STUDY DESIGN: The study comprised 33 consecutive patients admitted with suspected HFRS from April 2011-April 2016 at one center. Clinical diagnosis was confirmed by ELISA and real-time PCR. Statistical analysis was performed to identify prognostic markers and indicators of disease severity. RESULTS: The virus strain causing HFRS was Dobrava type in all 33 cases. The disease outbreaks occurred during the period June-July. Mean hospital stay was 15.7±6.9days. 29 (88%) of the patients were male. The mean age was 39.7±14.1. 16 (48.5%) patients were from Northeast Albania. 8 (24.2%) patients required dialysis. The strongest correlation was the inverse relationship of nadir platelet count with urea and creatinine, p<0.0001, p<0.0079 respectively. Creatinine and hyponatremia were inversely correlated p=0.0007, whereas hyponatremia and nadir platelet count had the highest sensitivity and specificity for development of severe AKI, 92.6%, 100%; 88.9%, 83.3% respectively. Mortality rate was 9.09%. CONCLUSION: HFRS is a severe viral disease in Albania caused by Dobrava strain. It is associated with high mortality, 9.09% in our cohort. In our study, thrombocytopenia, urinary volume, hyponatremia were indicators of more severe disease.