Oral health and related outcomes in children and adolescents with cystic fibrosis: a scoping review.

PURPOSE: Good oral health is important for children and adolescents with cystic fibrosis (CF). The purpose of this scoping review is to describe the existing evidence base regarding oral health in children and adolescents with CF and provide recommendations for future research. METHODS: Using a scoping review framework, a comprehensive search was undertaken using medline, embase, and PubMed. The search strategy included broad terms relating to CF, oral health, and children and adolescents and included only papers written in English. RESULTS: 61 articles were included. Topics investigated included dental caries, enamel defects, periodontal health, dental staining, oral health related quality of life, dental management, and dental development of children and adolescents with CF. CONCLUSION: Dental outcomes of children and adolescents with CF differ from the healthy population. The current literature describing dental health in children and adolescents with CF includes predominately descriptive analyses. A shift to hypothesis-based studies to explore causal relationships that explain the differences in dental outcomes seen in the CF population offers an opportunity to better understand the problems faced by children and adolescents with CF. Research that actively engages stakeholders, including children and adolescents with CF and their families will enable evidence-based recommendations to improve their oral health.

Multiple ocean parameter-based potential fishing zone (PFZ) location generation and validation in the Western Bay of Bengal.

A new conceptual framework based on satellite data, including chlorophyll (CHL), sea surface temperature (SST) fronts, relative winds, current vectors, Ekman transport, and eddies, has been developed to identify potential fishing zones (PFZ) in the Bay of Bengal (BoB). The framework aims to provide persistent forecasts, even under cloudy conditions, based on feature propagation. The validation of the PFZ was carried out using fish catch data collected by the Fishery Survey of India (FSI) between 2016 and 2018. Hooking rates (HR) from longlines and catch per unit effort (CPUE) from trawl nets were used to analyse the data points in hook rate categories (1.0-3.0 and > 3.0) and CPUE categories (50-100 kg and > 100 kg) and interpret them with the PFZ maps. The analysis showed that the high fish catch locations were consistent with persisting features in the BoB, such as high chlorophyll patches, SST fronts, and cyclonic eddies. The high fish catch locations based on hook rate and high CPUE were found to be collocated with the high chlorophyll persisting features and thermal gradients in the BoB. The regression analysis shows that availability of the food (CHL) had the strongest correlation with fish catch, followed by the comfort condition (fronts and eddies).

A new white-spotted moray eel, Gymnothorax aurocephalus sp. nov. (Muraenidae: Muraeninae) from Andaman Sea, India.

Gymnothorax aurocephalus sp. nov. is described herein based on 4 specimens. Three were collected from off Swaraj Dweep Island of Andaman and Nicobar Islands (AN Islands) from a depth of 125-130 m and one specimen off Interview Island, AN Islands at a depth of 90 m. This new species is distinguishable from its congeners by the following combination of characters: chocolate brown color overlain with small irregular white spots, golden skin shadings close to rictus; anus slightly before midbody; pointed and serrated jaw teeth; uniserial teeth in jaws and vomer; vertebral formula 7/61/148-149. The species is compared to all its congeners with white spots.

Developmental toxicity in embryo-larval zebrafish (Danio rerio) exposed to strobilurin fungicides (azoxystrobin and pyraclostrobin).

Azoxystrobin and pyraclostrobin are broad spectrum strobilurin fungicides that have been measured in the aquatic environment. Strobilurins inhibit mitochondrial respiration by binding to the mitochondrial respiratory complex III. The goal of this study was to investigate mitochondrial dysfunction and oxidative stress in the developing zebrafish from exposure to azoxystrobin and pyraclostrobin. Exposure studies were performed where zebrafish embryos were exposed to azoxystrobin and pyraclostrobin at 0.1, 10, 100 µg/L from 4 hpf to 48 hpf to measure mitochondrial dysfunction and oxidative stress mRNA transcripts, and 5 dpf to measure movement, growth, oxygen consumption, enzymatic activities, and mRNA transcripts. Results from this study indicated that there was a significant reduction in both basal and maximal respiration at 48 hpf in zebrafish exposed to 100 µg/L of pyraclostrobin. There was no difference in oxidative stress or apoptotic mRNA transcripts at 48 hpf, indicating that the two strobilurins were acting first on mitochondrial function and not directly through oxidative stress. At 5 dpf, standard body length was significantly reduced with exposure to pyraclostrobin and azoxystrobin exposure as compared to the control. These reductions in apical endpoints corresponded with increases in oxidative stress and apoptotic mRNA transcripts in treatment groups at 5 dpf indicating that strobilurins' exposure followed the adverse outcome pathway for mito-toxicants. Our results indicate that strobilurins can decrease mitochondrial function, which in turn lead to diminished growth and movement.

Further report of Bariaka alopiae Cressey, 1966 (Copepoda, Siphonostomatoida) from the Indian Ocean with new host and geographic record.

A copepod parasite, Bariaka alopiae Wilson, 1932 (Eudactylinidae) infested on thresher sharks caught from the Indian Exclusive Economic Zone off Andaman and Nicobar archipelago, eastern Indian Ocean is further reported. Adult females of B. alopiae were collected from the gill filaments of two host species, Alopias pelagicus Nakamura and A. superciliosus Lowe. The species B. alopiae can be easily distinguished from other species within the genus by the following characteristic features: cylindrical body devoid of spines, eighteen segmented antennules and four segmented abdomen. In the Indian Ocean, this parasite was known only from its original description 51 years ago, based on materials from western Indian Ocean off Madagascar. Further the present study reports, A. pelagicus as a new host for this parasite.

Comparative Lipid Peroxidation and Apoptosis in Embryo-Larval Zebrafish Exposed to 3 Azole Fungicides, Tebuconazole, Propiconazole, and Myclobutanil, at Environmentally Relevant Concentrations.

Azole fungicides have entered the aquatic environment through agricultural and residential runoff. In the present study, we compared the off-target toxicity of tebuconazole, propiconazole, and myclobutanil using embryo-larval zebrafish as a model. The aim of the present study was to investigate the relative toxicity of tebuconazole, propiconazole, and myclobutanil using multiple-level endpoints such as behavioral endpoints and enzymatic and molecular biomarkers associated with their mode of action. Zebrafish embryos were exposed to azoles at environmentally relevant and high concentrations, 0.3, 1.0, and 1000 µg/L, starting at 5 h postfertilization (hpf) up to 48 hpf, as well as 5 d postfertilization (dpf). Relative mRNA expressions of cytochrome P450 family 51 lanosterol-14α-demethylase, glutathione S-transferase, caspase 9, phosphoprotein p53, and BCL2-associated X protein were measured to assess toxicity attributable to fungicides at the mRNA level, whereas caspase 3/7 (apoptosis) and 3,4-methylene​dioxy​amphetamine (lipid peroxidation) levels were measured at the enzymatic level. Furthermore, mitochondrial dysfunction was measure through the Mito Stress test using the Seahorse XFe24 at 48 hpf. In addition, light to dark movement behavior was monitored at 5 dpf using Danio Vision® to understand adverse effects at the organismal level. There was no significant difference in the light to dark behavior with exposure to azoles compared to controls. The molecular biomarkers indicated that propiconazole and myclobutanil induced lipid peroxidation, oxidative stress, and potentially apoptosis at environmentally relevant concentrations (0.3 and 1 µg/L). The results from the mitochondrial respiration assay indicated a slight decrease in spare respiratory capacity with an acute exposure (48 hpf) to all 3 azoles at 1000 µg/L. Based on the present results, propiconazole and myclobutanil are acutely toxic compared to tebuconazole in aquatic organisms at environmentally relevant concentrations. Environ Toxicol Chem 2019;38:1455-1466. © 2019 SETAC.

Unusual complication of repair of fractured mandibular angle.

Internal fixation with miniplates remains an accepted and reliable approach to the management of mandibular fractures, and erosion through the oral mucosa is a well-recognised long-term complication. We report a case of erosion of a miniplate through the skin 5 years after internal fixation of a fracture of the left mandibular angle.

Bisphosphonate-associated osteonecrosis of the jaw: does it occur in children?

BACKGROUND: Bisphosphonate use in adult patients has been linked to osteonecrosis of the jaw (ONJ). This complication has not been systematically assessed in a paediatric population receiving bisphosphonates. OBJECTIVE: To assess our cohort of paediatric patients treated with intravenous bisphosphonate for occurrence of ONJ. DESIGN: Observational study at a tertiary children's hospital. PATIENTS: A total of 42 paediatric patients with osteoporosis who received bisphosphonate infusions for a mean of 6.5 years (SD 2.7 years) were assessed clinically and radiographically for possible ONJ. Among 42, 37 patients had received disodium pamidronate 1 mg/kg/dose at a mean cumulative dose of 19.8 mg/kg and zoledronic acid (ZA) 0.05 mg/kg/dose at a mean cumulative dose of 0.49 mg/kg; four had received ZA and one received pamidronate alone. Invasive dental treatment during bisphosphonate treatment, a known risk factor for osteonecrosis, was specifically assessed. RESULTS: In all patients assessed, including 11 who had invasive dental treatment, there were no cases of osteonecrosis. CONCLUSION: ONJ has so far not been demonstrated in this patient group.

Adding casein phosphopeptide-amorphous calcium phosphate to sports drinks to eliminate in vitro erosion.

PURPOSE: Enamel erosion can occur with frequent consumption of sports drinks. The purpose of this study was to determine a minimal concentration of casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP) which when added to a sports drink would eliminate such erosion in vitro. METHODS: Human enamel specimens were immersed in: (1) the sports drink Powerade; (2) Poweradeplus 4 concentrations of CPP-ACP (0.063%, 0.09%, 0.125%, 0.25%); or (3) double deionized water. Windows of test and control enamel were profiled, and the enamel surface characteristics were examined under scanning electron microscopy (SEM). RESULTS: The pH of test solutions increased and the titratable acidity decreased with increasing CPP-ACP concentrations. Erosive step lesions occurred in specimens immersed in Powerade (mean depth=38.70kA +/- 5.60), which were eliminated by the addition of CPP-ACP to Powerade at all test concentrations except 0.063% CPP-ACP. Microscopic surface irregularities on test enamel were observed, apparent as adherent granules or globules. These may represent redeposited mineral phases following mobilization of calcium and phosphate from CPP-ACP. Tasters in a taste panel could not distinguish Powerade from Powerade plus 0.125% CPP-ACP. CONCLUSIONS: Adding casein phosphopeptide-stabilized amorphous calcium phosphate to the sports drink Powerade significantly reduced the beverage's erosivity without affecting the product's taste.

Effect of various infection-control methods for light-cure units on the cure of composite resins.

This study (1) compared the curing-light intensity with various barrier infection-control methods used to prevent cross contamination, (2) compared the Knoop hardness value of cured composite resin when various barrier control methods were used, and (3) correlated the hardness of the composite resin with the light-intensity output when different infection-control methods were used. The light-cure unit tips were covered with barriers, such as cellophane wrap, plastic gloves, Steri-shields, and finger cots. The control group had no barrier. Composite resins were then cured for each of the five groups, and their Knoop hardness values recorded. The results showed that there was significant statistical difference in the light-intensity output among the five groups. However, there was no significant statistical difference in the Knoop hardness values among any of the groups. There was also no correlation between the Knoop hardness value of the composite resin with the light-intensity output and the different infection-control methods. Therefore, any of the five infection-control methods could be used as barriers for preventing cross-contamination of the light-cure unit tip, for the light-intensity output for all five groups exceeded the recommended value of 300 W/m2. However, to allow a greater margin of error in clinical situations, the authors recommend that the plastic glove or the cellophane wrap be used to wrap the light-cure tip, since these barriers allowed the highest light-intensity output.