Suppression of the Talbot effect in Fourier transform acousto-optic imaging.

We report on the observation and correction of an imaging artifact attributed to the Talbot effect in the context of acousto-optic imaging using structured acoustic waves. When ultrasound waves are emitted with a periodic structure, the Talbot effect produces π-phase shifts of that periodic structure at every half of the Talbot distance in propagation. This unwanted artifact is detrimental to the image reconstruction, which assumes near-field diffraction is negligible. Here, we demonstrate both theoretically and experimentally how imposing an additional phase modulation on the acoustic periodic structure induces a symmetry constraint leading to the annihilation of the Talbot effect. This will significantly improve the acousto-optic image reconstruction quality and allows for an improvement of the reachable spatial resolution of the image.

Optical coherent detection through multi-scattering media by wave-mixing cleaning effect in liquid-crystal OASLM.

Liquid-crystal (LC) optically addressable spatial light modulators (OASLMs) allow control of the phase and amplitude of optical beams. By performing wave mixing in an OASLM, we show that coherent phase detection can be achieved for light beams passing through highly scattering media, such as foam layers with several cm thicknesses. Thanks to the adaptive response of our OASLM, the phase information on the speckle signal is transferred at the output of the OASLM to the plane wave reference beam, allowing the cleaning of optical distortions and the direct measurement of amplitude phase modulations with a small diameter single photodiode. A good signal-to-noise ratio (SNR) is demonstrated for foam thickness up to 3 cm. These properties, together with the recently demonstrated sub-ms response time of our OASLM, make the method compatible with foreseen applications for imaging in biomedical tissues and turbid media.

In vivo ultrasound modulated optical tomography with a persistent spectral hole burning filter.

We present in vivo ultrasound modulated optical tomography (UOT) results on mice, using the persistent spectral hole burning (PSHB) effect in a Tm3+:YAG crystal. Indocyanine green (ICG) solution was injected as an optical absorber and was clearly identified on the PSHB-UOT images, both in the muscle (following an intramuscular injection) and in the liver (following an intravenous injection). This demonstration also validates an experimental setup with an improved level of performance combined with an increased technological maturity compared to previous demonstrations.

Fourier transform acousto-optic imaging with off-axis holographic detection.

Acousto-optic (AO) imaging is an in-depth optical imaging technique of highly scattering media. One challenging end-application for this technique is to perform imaging of living biological tissues. Indeed, because it relies on coherent illumination, AO imaging is sensitive to speckle decorrelation occurring on the millisecond time scale. Camera-based detections are well suited for in vivo imaging provided their integration time is lower than those decorrelation time scales. We present Fourier transform acousto-optic imaging combined with off-axis holography, which relies on plane waves and long-duration pulses. We demonstrate, for the first time to the best of our knowledge, a two-dimensional imaging system fully compatible with in vivo imaging prerequisites. The method is validated experimentally by performing in-depth imaging inside a multiple scattering sample.

Reconstruction of bi-dimensional images in Fourier-transform acousto-optic imaging.

We present a new, to the best of our knowledge, method to perform acousto-optic imaging based on a spatiotemporal structuration of long-duration acoustic plane waves. This approach is particularly relevant when using detectors with long integration times. We show how it is possible to reconstruct an image by measuring its two-dimensional Fourier components. A proof of concept is presented using a photorefractive detection scheme, demonstrating equal performances to direct imaging. The overall acquisition time is compatible with medical monitoring applications.

Structured ultrasound-modulated optical tomography.

Ultrasound-modulated optical tomography (UOT) is an imaging technique that couples light and ultrasound in order to perform in-depth imaging of highly scattering media. In previous work, we introduced plane wave UOT, an imaging method analogous to x-ray tomography based on the filtered backprojection for image reconstruction. Angle-limited measurements, however, led to drastic loss of lateral spatial resolution. Here, we present a new structured ultrasonic plane wave UOT method that allows partial recovery of the resolution. For image reconstruction, we present a generalization of the Fourier slice theorem along with a generalized filtered backprojection formalism. The method is successfully tested on simulated and experimental data.

Controlling light in complex media beyond the acoustic diffraction-limit using the acousto-optic transmission matrix.

Studying the internal structure of complex samples with light is an important task but a difficult challenge due to light scattering. While the complex optical distortions induced by scattering can be effectively undone if the medium's scattering-matrix is known, this matrix generally cannot be retrieved without the presence of an invasive detector or guide-star at the target points of interest. To overcome this limitation, the current state-of-the-art approaches utilize focused ultrasound for generating acousto-optic guide-stars, in a variety of different techniques. Here, we introduce the acousto-optic transmission matrix (AOTM), which is an ultrasonically-encoded, spatially-resolved, optical scattering-matrix. The AOTM provides both a generalized framework to describe any acousto-optic based technique, and a tool for light control and focusing beyond the acoustic diffraction-limit inside complex samples. We experimentally demonstrate complex light control using the AOTM singular vectors, and utilize the AOTM framework to analyze the resolution limitation of acousto-optic guided focusing approaches.

Ultrasound-modulated optical tomography in scattering media: flux filtering based on persistent spectral hole burning in the optical diagnosis window.

Ultrasound-modulated optical tomography (UOT) is a powerful imaging technique to discriminate healthy from unhealthy biological tissues based on their optical signature. Among the numerous detection techniques developed for acousto-optic imaging, only those based on spectral filtering are intrinsically immune to speckle decorrelation. This Letter reports on UOT imaging based on spectral hole burning in Tm:YAG crystal under a moderate magnetic field (200G) with a well-defined orientation. The deep and long-lasting holes translate into a more efficient UOT imaging with a higher contrast and faster imaging frame rate. We demonstrate the potential of this method by imaging calibrated phantom scattering gels.

Two-color interpolation of the absorption response for quantitative acousto-optic imaging.

Diffuse optical tomography (DOT) is a reliable and widespread technique for monitoring qualitative changes in absorption inside highly scattering media. It has been shown, however, that acousto-optic (AO) imaging can provide significantly more qualitative information without the need for inversion algorithms due to the spatial resolution afforded by ultrasound probing. In this Letter, we show how, by using multiple-wavelength AO imaging, it is also possible to perform quantitative measurements of absorber concentration inside scattering media.

Refractive index and gain grating in Nd:YVO4: application to speckle vibrometry and photoacoustic detection.

Probing local displacements on a scattering surface can be achieved using an adaptive interferometer. The photorefractive-crystal-based interferometer is popular, but alternatives exist such as an adaptive gain interferometer. Such setups take advantage of the nonlinear phenomena in laser media. Because of the gain saturation, it is possible to write a gain hologram and a refractive index hologram to achieve an adaptive interferometer with a linear response. In addition, laser-media-based setups have a fast response time (≤100 µs), which makes them interesting for applications such as detection of photoacoustic waves in living biological samples.

Theoretical study of Fourier-transform acousto-optic imaging.

We propose a full theoretical study of Fourier-transform acousto-optic imaging, which we recently introduced and experimentally assessed in [Opt. Lett.40, 705-708 (2015)OPLEDP0146-959210.1364/OL.40.000705] as an alternative to achieve axial resolution in acousto-optic imaging with a higher signal-to-noise ratio.

Ultrafast acousto-optic imaging with ultrasonic plane waves.

Due to multiple light scattering inside biological tissues, deep non-invasive optical medical imaging is very challenging. Acousto-optic imaging is a technique coupling ultrasound and light that allows recovering optical contrast at depths of few centimeters with a millimeter resolution. Recent advances in acousto-optic imaging are using short focused ultrasound pulses often averaged over several hundred or thousand pulses. As the pulsing rate of commercial probes is limited to about few ultrasound cycles every 100 µs, acquiring an acousto-optic image usually takes several tens of seconds due to the high number of acoustic pulses excitation. We propose here a new acousto-optic imaging technique based on the use of ultrasound plane waves instead of focused ones that allows increasing drastically the imaging rate.

Revealing the Cu(2+) ions localization at low symmetry Bi sites in photorefractive Bi12GeO20 crystals doped with Cu and V by high frequency EPR.

The sites of incorporation of Cu(2+) impurity ions in Bi12GeO20 single crystals co-doped with copper and vanadium have been investigated by electron paramagnetic resonance (EPR). While the X-band EPR spectra consist of a simple broad (ΔB ∼50 mT) line with anisotropic lineshape, the W-band EPR spectra exhibit well resolved, strongly anisotropic lines, due to transitions within the 3d(9)-(2)D ground manifold of the Cu(2+) ions. The most intense group of lines, attributed to the dominant Cu(2+)(I) center, displays a characteristic four components hyperfine structure for magnetic field orientations close to a 〈110〉 direction. The g and A tensor main axes are very close to one of the 12 possible sets of orthogonal 〈1-10〉, 〈00-1〉 and 〈110〉 crystal directions. Several less intense lines, with unresolved hyperfine structure and similar symmetry properties, mostly overlapped by the Cu(2+)(I) spectrum, were attributed to Cu(2+)(II) centers. The two paramagnetic centers are identified as substitutional Cu(2+) ions at Bi(3+) sites with low C1 symmetry, very likely resulting from different configurations of neighboring charge compensating defects.

Fourier transform acousto-optic imaging with a custom-designed CMOS smart-pixels array.

We report acousto-optic imaging (AOI) into a scattering medium using a Fourier Transform (FT) analysis to achieve axial resolution. The measurement system was implemented using a CMOS smart-pixels sensor dedicated to the real-time analysis of speckle patterns. This first proof-of-principle of FT-AOI demonstrates some of its potential advantages, with a signal-to-noise ratio comparable to the one obtained without axial resolution, and with an acquisition rate compatible with a use on living biological tissue.

Multi-modal acousto-optic/ultrasound imaging of ex vivo liver tumors at 790 nm using a Sn2 P2 S6 wavefront adaptive holographic setup.

Biological tissues are very strong light-scattering media. As a consequence, current medical imaging devices do not allow deep optical imaging unless invasive techniques are used. Acousto-optic imaging is a light-ultrasound coupling technique that takes advantage of the ballistic propagation of ultrasound in biological tissues to access optical contrast with a millimeter resolution. We have developed a photorefractive-crystal-based system that performs self-adaptive wavefront holography and works within the optical therapeutic window. As it works at an appropriate wavelength range for biological tissues imaging, it was tested on ex vivo liver samples containing tumors as a pre-clinical study. Optical contrast was obtained even if acoustical one was not significant. Ultrasound image (left) and acousto-optic image (right) of a liver biopsy with tumors. Acousto-optic imaging exhibits tumors that are not detected through ultrasound.

Modelization and optimized speckle detection scheme in photorefractive self-referenced acousto-optic imaging.

A photorefractive BSO single crystal can be used for axially resolved acousto-optic imaging of thick scattering media in absence of a reference beam. This configuration renders the experimental setup easier to realize for imaging through thick scattering media with an improved optical etendue. We present here a model and simulations that explains these results. It is based on the spatial heterogeneity of the speckle pattern incident on the crystal. Optimization of the detector position and of the speckle grain size is confirmed by the model.

Experimental study of z resolution in acousto-optical coherence tomography using random phase jumps on ultrasound and light.

Acousto-optical coherence tomography (AOCT) is a variant of acousto-optic imaging (also called ultrasound modulated optical tomography) that makes possible to get resolution along the ultrasound propagation axis z. We present here AOCT experimental results, and we study how the z resolution depends on time step between phase jumps T(φ), or on the correlation length Δz. By working at low resolution, we perform a quantitative comparison of the z measurements with the theoretical point spread function. We also present images recorded with different z resolution, and we qualitatively show how the image quality varies with T(φ), or Δz.

Dynamic ultrasound modulated optical tomography by self-referenced photorefractive holography.

Photorefractive Bi(12)SiO(20) single crystal is used for acousto-optic imaging in thick scattering media in the green part of the spectrum, in an adaptive speckle correlation configuration. Light fields at the output of the scattering sample exhibit typical speckle grains of 1 µm size within the volume of the nonlinear crystal. This heterogeneous illumination induces a complex refractive index structure without applying a reference beam on the crystal, leading to a self-referenced diffraction correlation scheme. We demonstrate that this simple and robust configuration is able to perform axially resolved ultrasound modulated optical tomography of thick scattering media with an improved optical etendue.

Acousto-optical coherence tomography with a digital holographic detection scheme.

Acousto-optical coherence tomography (AOCT) consists in using random phase jumps on ultrasound and light to achieve a millimeter resolution when imaging thick scattering media. We combined this technique with heterodyne off-axis digital holography. Two-dimensional images of absorbing objects embedded in scattering phantoms are obtained with a good signal-to-noise ratio. We study the impact of the phase modulation characteristics on the amplitude of the acousto-optic signal and on the contrast and apparent size of the absorbing inclusion.

Time resolved three-dimensional acousto-optic imaging of thick scattering media.

Acousto-optic imaging is based on light interaction with focused ultrasound in a scattering medium. Thanks to photorefractive holography combined with pulsed ultrasound, we perform a time-resolved detection of ultrasound-modulated photons in the therapeutic window (780 nm). A high-gain SPS:Te crystal is used for this purpose and enables us to image through large optical thickness (500 mean free paths). We are able to generate three-dimensional (3D) acousto-optic images by translating a multielement ultrasound probe in only one direction. A 3D absorbing object is imaged through a 3 cm thick phantom.