ABSTRACT
Purpose: The aim of the present study is to determine the amount of titanium ions released into the artificial salivary medium by modified glass ionomer cement (GIC) doped with 3% and 5% (w/w) titanium dioxide nanoparticles (TiO2-NPs), and to evaluate their antibacterial properties. Materials and methods: 120 cylindrical discs with a diameter of 4 mm and a height of 6 mm were made with 3% and 5% w/w modified GIC containing TiO2 NPs, divided into two groups of 60, and immersed in a chemically synthesized salivary medium. The samples were quantified over four-time periods: 24 hours, two months, four months, and six months, using inductively coupled plasma mass spectroscopy (ICP-MS), antibacterial properties were evaluated by means of colony forming count (CFU) method. Results: The amount of titanium ions released from the discs that received 3%(w/w) TiO2 was highest in the first two months, with no significant release at successive intervals. Also, the second group, which included 5% (w/w) TiO2, saw a considerable ion release at every interval, with the second month seeing the maximum release. The levels in the 5% (w/w) group were consistently higher when the two concentrations were compared at each of the four time points, indicating a considerable increase in titanium release and antibacterial property with a concentration increase from 3% to 5%. Conclusion: 3% and 5% (w/w) concentrations may be considered safe and exhibit significant antimicrobial effect, titanium ions were discharged at higher rates in 5% (w/w) modified GIC containing TiO2-NPs than in 3% (w/w) modified GIC containing TiO2-NPs.
ABSTRACT
BACKGROUND: To evaluate the clinical non-inferiority of injectable platelet-rich fibrin and micro-needling (iPRF+MN) with free gingival grafting (FGG) in periodontal phenotype modification therapy. METHODOLOGY: Twenty participants with a gingival thickness (GT)<0.8 mm in the mandibular anterior region were included. The participants were randomly allocated into i-PRF+MN and FGG groups. i-PRF injection was done, and MN was done with the help of a lancet in the i-PRF+MN group. The clinical parameters, i.e., GT and Keratinized tissue width (KTW), were evaluated at baseline, 3 and 6 months post-operatively. Patient-reported outcomes (PROs), i.e., discomfort and esthetic satisfaction, were assessed using the visual analogue score(VAS) at one week and 3 months of re-evaluation, respectively. RESULTS: Both groups showed a significant increase in GT and KTW with no intergroup variation (P-0.32, 0.48respectively) at the end of 6 months. However, the i-PRF+ MN group showed better PROs, i.e., less discomfort(1.11±0.60) and better esthetic satisfaction(8.77±0.44). CONCLUSION: The non-invasive treatment of i-PRF+MN may lead to non-inferior clinical outcomes after 6 months with better PROs compared to more invasive FGG protocols in phenotype modification therapy.
ABSTRACT
BACKGROUND: A biological seal that protects the implant from any biological or external impingement is created by the supracrestal attached tissues. Sohn's poncho technique is a technique that utilizes a healing abutment at the implant site to stabilize the platelet rich fibrin (PRF) membrane. Thus, the aim of this study is to evaluate the efficacy of Sohn's poncho technique used for placement of leukocyte PRF (L-PRF) membrane in improving the peri-implant mucosal thickness and width of keratinized mucosa as well as in the acceleration of healing process compared to the peri-implant mucosa surrounding healing abutments placed without the L-PRF membrane. METHODS: A split mouth randomized controlled clinical trial was designed in which implants were placed in the mandibular posterior region. Healing abutment is placed along with the L-PRF membrane at the test site using Sohn's poncho technique and at control site conventional healing abutment placement was done at second stage. The thickness of peri-implant mucosa as primary outcome and the Width of keratinized tissue and healing as secondary outcomes were measured and assessed at various time intervals. RESULTS: Statistically significant difference was seen in inter-group analysis when peri-implant mucosal thickness (3.8 ± 0.4 mm vs. 2.3 ± 0.4 mm) and width of keratinized mucosa (3.6 ± 0.6 mm vs. 2.7 ± 0.3 mm) in test and control groups respectively and intragroup analysis of test and control groups at 4 weeks and 6 weeks' time points. The control group showed faster healing when compared to the test group. CONCLUSION: Sohn's poncho technique in combination with L-PRF has the potential to improve the thickness of peri-implant mucosa and the width of keratinized mucosa around implants.
ABSTRACT
Background: Peri-implantitis can be attributed to many underlying causes, one of the chief ones being due to infection caused by oral micro flora and particularly Aggregatibacter actinomycetemcomitans. Antibiotics are administered along with mechanical debridement to control the infection. The side effect of conventional antibiotic therapy and drug resistance has led to the necessity for alternate approaches to handle infections. Natural products are being investigated because of their multi-target activity and structurally different from the normal antibiotics. Propolis a product by Apis Mellifera bees as a wound healing and bone regenerating effect along with antimicrobial effect. One of the important features of Propolis is the chemical properties of Propolis changes with the different locations of procurement. Antimicrobial activity of Hubballi propolis against Aggregatibacter actinomycetemcomitans is not been reported in the literature. Aim: The aim of this study is to evaluate the antimicrobial effect of the Hubballi Propolis against Aggregatibacter actinomycetemcomitans. Methods: The two solvents used for the study were water and 70% Aq ethanol. Minimum inhibitory concentration (MIC), total phenolic contents (TPC), and total flavonoid content (TFC) were tested. Results: Hubballi Propolis sample showed antimicrobial effect against Aggregatibacter actinomycetemcomitans with MIC range from 0.1 mg/ml to 0.25 mg/ml. Conclusion: Hubballi Propolis is effective against Aggregatibacter actinomycetemcomitans infection thus may help in treating peri-implantitis. Propolis extracted with water as solvent showed better MIC, higher TPC and TFC than the propolis extracted using alcohol as solvent. This feature is noteworthy as the formulations produced using water extract is favorable than alcohol extract of propolis which irritates the mucosa and hence difficult for its application in dentistry.
ABSTRACT
BACKGROUND: The present study is aimed at analyzing the expression of inducible nitric oxide synthase (iNOS) in the epithelial lining of odontogenic keratocyst (OKC), dentigerous cyst (DC), radicular cyst (RC) in order to understand the possible role of iNOS with special reference to its neoplastic nature and local aggressive of cysts. AIM: The primary aim of the following study is to analyze the immunohistochemical expression of iNOS and secondary aim is to compare the iNOS expression, pattern and intensity of staining among the epithelial linings of OKC, DC and RC. MATERIALS AND METHODS: iNOS in the epithelial lining cells were analyzing in 10 OKC's, 10 DC's and 10 RC's using immunohistochemistry. The percentage of positive cells was assessed and presented as mean ± standard deviation. The correlation with respect to the intensity and percentage of staining within the epithelial linings of OKCs, DCs and RCs was carried out using (analysis of variance and Student's t-test) Chi-square test. RESULTS: Staining intensity of iNOS portion was seen in the entire thickness of the epithelial linings of OKC, whereas in DC's only one case had entire thickness of the epithelial lining staining and in RC's none of the cases showed entire thickness of staining. On comparing the staining intensity of iNOS between OKC, DC and RC groups, using Chi-square test, there was a statistically significant difference between these groups (P < 0.01). On analyzing the immuno-reactivity of iNOS in OKC, DC and RC there was a positive variable expression iNOS between the cysts. CONCLUSION: iNOS was over expressed in OKCs when compared with DC and RC suggesting that iNOS may contribute to the aggressive behavior of OKC. This is yet another evidence to support that OKC is the neoplasm.
