Permeability Data of Organosulfur Garlic Compounds Estimated by Immobilized Artificial Membrane Chromatography: Correlation Across Several Biological Barriers.

Among healthy vegetables, those of the genus Allium stand out. Antioxidant and anti-inflammatory properties have been associated with these vegetables, attributed mainly to organosulfur compounds (OSCs). In turn, they are linked to a protective effect counteracting cardiovascular disease development. Now, to really ensure the bioactive efficacy of the said compounds once consumed, it is necessary to previously evaluate the ADME (absorption, distribution, metabolism, and excretion) profile. Alternatively, in vitro and in silico methods attempt to avoid or reduce experimental animals' use and provide preliminary information on drugs' ability to overcome the various biological barriers inherent in the ADME process. In this sense, in silico methods serve to provide primary information on drugs' bioavailability mechanisms. High-performance liquid chromatography (HPLC) using a stationary phase composed of phospholipids, the so-called immobilized artificial membrane (IAM), has been widely recognized as a valuable alternative method to extract and quantify information about the structure and physicochemical properties of organic compounds which are extensively used in studies of quantitative structure-activity relationships (QSARs). In the present study, the chromatographic capacity factors (log k' (IAM)) for 28 OSCs were determined by IAM-HPLC. In order to evaluate the ability of the IAM phase in assessing lipophilicity of the compounds under study, several quantitative structure-retention relationships (QSRRs) were derived from exploring fundamental intermolecular interactions that govern the retention of compounds under study on IAM phases. As expected, the hydrophobic factors are of prime importance for the IAM retention of these compounds. However, the molecular flexibility and specific polar interactions expressed by several electronic descriptors (relative negative charge, RNCG, and Mulliken electronegativity) are also involved. We also evaluated the IAM phase ability to assess several ADME parameters for the OSCs under study obtained using the SwissADME web tool integrated into the SwissDrugDesign workspace and the PreADMET web tool. The human gastrointestinal absorption (HIA), blood-brain barrier (BBB) permeation, and skin permeability were investigated through QSAR modeling, using several chemometric approaches. The ADME properties under study are strongly dependent on hydrophobic factors as expressed by log k'(IAM), which provide evidence for the great potential of the IAM phases in the development of QSAR models.

Multi-phytochemical determination of polar and non-polar garlic bioactive compounds in different food and nutraceutical preparations.

Amongst functional foods, garlic and its by-products stand out given their rich phytochemical profile. A comprehensive analytical approach becomes necessary to fully address garlic preparations health-promoting activities, considering the coexistence of several active ingredients from different chemical families. For this, we developed a multi-phytochemical protocol combining Ultrasound and Dispersive Liquid-Liquid Microextraction, coupled to Liquid Chromatography, for the determination of flavonols, organosulfur compounds, and inulin. Hydrophilic interaction chromatography showed an adequate resolution of flavonols and sugars in a shorter time. The protocol showed a suitable performance and acceptable quantitative yields for garlic powder, cooked garlic, black garlic, and liquid garlic flavouring samples. Additionally, the proposed methodology represented a useful tool to assess how the different garlic products related to functional properties, taking into account the various phytochemical families present in each sample. This is the first time a comprehensive and multi-phytochemical validated analysis of garlic preparations is proposed.

Evaluación de la fuerza muscular en niños: una revisión de la literatura / Evaluation of muscle strength in children: a review of literature

Introducción: la fuerza muscular es un indicador importante de la función neuromuscular y un componente de la aptitud física esencial para el desempeño en actividades cotidianas. Su alteración puede causar deficiencias funcionales, afectar la independencia y los roles que desempeña una persona. Por esto, evaluar la fuerza muscular en niños es necesario para determinar el grado de rendimiento motor e identificar riesgos asociados al crecimiento y al desarrollo. Objetivo: describir el estado del arte relacionado con la utilización de escalas de valoración de la fuerza muscular en niños entre 3 y 8 años de edad. Materiales y Métodos: se realizó una revisión de la literatura empleando la estrategia PICO, con búsqueda bibliográfica en las bases de datos Ovid, Sage, Science Direct, Proquest y Clinical Key. Resultados: en la fase inicial del proceso de cribado se identificaron 69 registros elegibles por título y resumen, de los cuales se excluyeron 56 por no cumplir con los criterios de inclusión. Adicionalmente se identificaron 3 libros con información relevante, razón por la cual fueron tenidas en cuenta 16 referencias que hacen parte de la síntesis cualitativa del presente estudio. Conclusiones: estrategias y pruebas como la dinamometría, la Functional Strength Measurement, el Test de Competencias Motoras de Bruininks Oseretsky, el Fitness Testing Preschool Children y FUPRECOL son herramientas que permiten evaluar la fuerza muscular en la infancia, y aportan elementos importantes en la identificación temprana de factores de riesgo para la salud de la persona..Au

Introduction: muscle strength is an important indicator of neuromuscular function and a component of physical fitness essential for performance in different activities of daily life. Its alteration may cause functional limitations and affects the independence and the roles that a person develops. That is why the evaluation of muscle strength is necessary to determine the level of motor performance and identify risks associated with growth and development. Objective: to describe the state of art related to the use of muscle strength assessment scales in children between 3 and 8 years of age. Materials and Methods: a review of the literature was performed using the PICO strategy, with bibliographic search in the Ovid, Sage, Science Direct, Proquest and Clinical Key databases. Results: in the initial phase of the screening process, 69 eligible records were identified by title and abstract, of which 56 were excluded because they did not meet the inclusion criteria. Additionally, 3 books with relevant information were identified, which is why 16 references that are part of the qualitative synthesis of this study were taken into account. Conclusions: srategies and tests such as dynamometry, Functional Strength Measurement, Bruininks Oseretsky's Motor Skills Test, Fitness Testing Preschool Children and FUPRECOL are tools that allow evaluating muscle strength in childhood, and provide important elements in early identification of risk factors for the person's health..Au

Development of garlic bioactive compounds analytical methodology based on liquid phase microextraction using response surface design. Implications for dual analysis: Cooked and biological fluids samples.

Organosulphur compounds (OSCs) present in garlic (Allium sativum L.) are responsible of several biological properties. Functional foods researches indicate the importance of quantifying these compounds in food matrices and biological fluids. For this purpose, this paper introduces a novel methodology based on dispersive liquid-liquid microextraction (DLLME) coupled to high performance liquid chromatography with ultraviolet detector (HPLC-UV) for the extraction and determination of organosulphur compounds in different matrices. The target analytes were allicin, (E)- and (Z)-ajoene, 2-vinyl-4H-1,2-dithiin (2-VD), diallyl sulphide (DAS) and diallyl disulphide (DADS). The microextraction technique was optimized using an experimental design, and the analytical performance was evaluated under optimum conditions. The desirability function presented an optimal value for 600µL of chloroform as extraction solvent using acetonitrile as dispersant. The method proved to be reliable, precise and accurate. It was successfully applied to determine OSCs in cooked garlic samples as well as blood plasma and digestive fluids.