ABSTRACT
Egyptian henbane (Hyoscyamus muticus L. Mant. Pl. 1767) is an important medicinal plant of the family Solanaceae. Its complete chloroplast (cp) genome was assembled using Illumina high-throughput sequencing technology to contribute to its conservation genetics studies. Here, we report the complete sequence of the chloroplast genome of H. muticus. The cp genome was 156,271 bp in length with an asymmetric base composition (30.9% A, 18.9% C, 18.6% G and 31.6% T). It encodes 132 genes comprising 87 protein-coding genes, 29 tRNA genes, and 8 rRNA genes. The overall GC content of the H. muticus chloroplast genome was 37.5%, and the corresponding values in the large single-copy (LSC), the small single-copy (SSC), and the inverted repeat (IR) regions were 35.5%, 31.5%, and 43.0%, respectively. The complete chloroplast genome sequence was deposited to the GenBank (NCBI, Accession number: MZ450974). The maximum likelihood phylogenetic analysis showed that the H. muticus and H. niger were clustered into one clade with strong support values, indicating their closer relationship.
ABSTRACT
The aim of the present study was to analyze the chemical composition of Curcuma longa, Pimenta dioica, Rosmarinus officinalis, and Syzygium aromaticum essential oils (EOs) and their antifungal and anti-conidiogenic activity against Fusarium verticillioides. The chemical profile of the EOs was determined by GC/MS. The antifungal and anti-conidiogenic activities were evaluated by the agar dilution method. The tested concentrations were 1000ppm, 500ppm, 250ppm and 125ppm. S. aromaticum EO exhibited the highest antifungal effect, followed by P. dioica and to a lesser extent C. longa. The major compounds of these EOs were eugenol (88.70% in S. aromaticum and 16.70% in P. dioica), methyl eugenol (53.09% in P. dioica), and α-turmerone (44.70%), ß-turmerone (20.67%), and Ar-turmerone (17.27%) in C. longa. Rosmarinus officinalis poorly inhibited fungal growth; however, it was the only EO that inhibited conidial production, with its major components being 1,8-cineole (53.48%), α-pinene (15.65%), and (-)-camphor (9.57%). Our results showed that some compounds are capable of decreasing mycelial growth without affecting sporulation, and vice versa. However, not all the compounds of an EO are responsible for its bioactivity. In the present work, we were able to identify different major compounds or mixtures of major compounds that were responsible for antifungal and anti-conidiogenic effects. Further experiments combining these pure components are necessary in order to achieve a highly bioactive natural formulation against the phytopathogenic fungus F. verticillioides.
