ABSTRACT
Introduction Dental biofilm constitutes micro-organisms existing in an intercellular matrix containing organic and inorganic materials derived from saliva, gingival crevicular fluid, and bacterial products. Dental plaque biofilm inhibition by certain herbs and medicinal plants has been used as a treatment modality for the prevention of white spot lesions in orthodontic subjects. The aim of this study was to evaluate the anti-quorum sensing and anti-biofilm activity of Terminalia catappa and Murraya koenigiiagainst Streptococcus mutans. Materials and methods Samples of dental plaque were taken from patients receiving orthodontic care. The colonies of the S. mutans were isolated and biochemical characterization was done. Leaf extracts of Terminalia catappa and Murraya koenigii were used in the study. Methanolic extracts were subjected to evaluation of minimum inhibitory concentration (MIC) using the broth microdilution (two-fold) method and anti-biofilm activity using the crystal violet staining method. Results The MIC of methanol leaf extracts of Murraya koenigii against S. mutans was noted at 0.62 mg/ml and Terminalia catappa at 1.25 mg/ml. At the lowest concentration of 0.03 mg and 0.01 mg methanol extract of Murraya koenigii had remarkably inhibited biofilm formation of 57.6% and 43.6% against S. mutans, respectively. Terminalia catappa leaf extracts did not show any anti-biofilm activity when the organisms were grown in the presence of S. mutans. Conclusion Both Murraya koenigii and Terminalia catappa had antibacterial effects against S. mutans and Murray koenigii remarkably inhibited biofilm formation by S. mutans.
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Introduction Dental plaque biofilms are a collection of microorganisms that are adhered to the tooth enamel surface. Inhibition of plaque biofilms is required to prevent dental caries and periodontitis and currently, there are many chemical and herbal products in use for inhibition of biofilms but with limited success. Materials and methods Dental plaque collection was done from subjects undergoing orthodontic therapy followed by isolation of Streptococcus mutans. Isolated S. mutans were subjected to disk diffusion assay with 4-HCA (baseline 10mg/mL) for the zone of inhibition and broth micro-dilution to evaluate the minimum inhibitory concentration (MIC) and sub-MIC. Crystal violet staining was done for biofilm inhibition assay. Results Growth of S. mutans was inhibited by 4-HCA at concentrations as low as 0.31 mg/mL. 4-HCA (40µL) inhibited the bacterial growth and a clear zone (15 mm) was observed. 4-Hydroxycinnamic acids treated culture showed progressive reduction in the biofilm production at the concentration of 0.01 mg/mL. The 4-HCA concentration as low as 4 mg and 2 mg has remarkably inhibited biofilm formation of 49.3% and 34.3%, respectively. Conclusion The anti-quorum sensing and anti-biofilm activity of 4-Hydroxycinnamic acid against S. mutans isolated from subjects undergoing orthodontic treatment showed a remarkable result.
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Introduction Class II malocclusions are commonly associated with some muscle disharmony and imbalance. Diagnosis of muscle imbalance helps in treating the malocclusion as well as preventing relapse of the treatment. The aim of this study is to compare the muscle activity of masseter and temporalis in patients with skeletal Class II division 1 malocclusion with varying overjet using surface electromyography (sEMG). Materials and methods Ten subjects in the age range 18-35 years with skeletal Class II malocclusion and varying overjets who required orthodontic treatment were included in this study. Out of these 10 patients, five of them had a 2-4mm overjet and the other five had an overjet >4mm. A four-channel sEMG system was used to conduct the sEMG of muscles. Muscle activity, synergy, and symmetry of masseter and temporalis muscles were assessed and compared between the two groups with an Independent t-test. Results There were no significant differences in the muscle activities of the temporalis and masseter muscles in both groups. Symmetry and synergy of these muscles in the two groups also showed no significant difference (p>0.05) at rest and clenching. However, during chewing, the masseter muscle showed poor balance and activity. Conclusion The overjet in Class II division 1 malocclusions did not seem to affect the muscle activity at rest and during clenching. In patients with increased overjet, during chewing, masseter activity in terms of intensity and balance was poor.
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Background: Bonding is an important step in fixed orthodontic therapy and evaluation of bracket bond failures while using different bonding systems is required. The aim of the present study was to evaluate and compare bracket failure rates of a novel no primer adhesive with conventional primer-based orthodontic adhesives. Methods: This split mouth study was conducted among fifteen patients who underwent therapy with fixed orthodontic appliances using metal brackets. Total of 300 brackets were bonded and the bracket bond failure rates were assessed at the end of 3 months. The difference in bond failure rates between the two groups were assessed in different teeth. Descriptive statistics and chi-square test was performed. Results: Evaluation of bracket bond failure rates showed a higher incidence of bond failures in the group bonded with the primerless adhesive (6.3%) compared to conventional adhesive (2.3%) but there was no statistically significant difference (P>0.05). No intergroup difference was found in the bracket failure rates of individual teeth (P>0.05). Conclusion: Higher incidence of bond failures were noted with brackets bonded with primerless adhesive when compared to primer-based adhesive but no significant difference was noted over a period of 3 months. Mandibular canine and premolars had a higher bracket failure rate with no significant difference between the adhesives.
ABSTRACT
Aims and Objectives: Mini screws placed buccal to the maxillary first or second molars in the infra zygomatic crest (IZC) region can be used as anchors for various types of tooth movement. En masse distal movement of the maxillary dentition with IZC anchorage is routinely practiced nowadays as more patients demand a non-extraction treatment and it should be evaluated. The goal of this study was to assess dentoalveolar and airway changes in individuals with class II malocclusion after en masse distal movement of the maxillary dentition utilizing infrazygomatic anchorage. Materials and Methods: This prospective study included patients who required en masse distal movement of the maxillary dentition. Following initial leveling and aligning, mini screws were placed in the IZC region, and the maxillary arch was distalized en-masse. Pre (T0) and post distalization (T1) lateral cephalograms were traced for dentoalveolar and airway changes. Statistical tests were done with SPSS software. Shapiro-Wilk test for normality and paired T test for comparison between before and after en masse distalization were done. Results: The changes in dental angular and linear measurements such as U1 to N-A, L1 to N-B and interincisal angle, U1 to N-A and U1 to point A distance, U1 to palatal plane, L1 to N-B, L1 to Apo line distance, U6 to PtV were statistically significant (P > 0.05). Linear parameters such as L1 to ApO line, upper airway, and lower airway were not statistically significant (<0.05). Conclusion: Class II div I malocclusions can be efficiently corrected without extractions using IZC anchorage by en masse distal movement of the maxillary dentition. Significant reduction in upper anterior inclination, intrusion of maxillary anterior teeth, and distal movement of the posterior teeth were noted. No changes in airway dimensions were noted.
