Differential expression of long non-coding RNAs under Peste des petits ruminants virus (PPRV) infection in goats.

Peste des petits ruminants (PPR) characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, is an acute, highly contagious viral disease of sheep and goats. The role of long non-coding RNAs (lncRNAs) in PPRV infection has not been explored to date. In this study, the transcriptome profiles of virulent Peste des petits ruminants virus (PPRV) infected goat tissues - lung and spleen were analyzed to identify the role of lncRNAs in PPRV infection. A total of 13,928 lncRNA transcripts were identified, out of which 170 were known lncRNAs. Intergenic lncRNAs (7625) formed the major chunk of the novel lncRNA transcripts. Differential expression analysis revealed that 15 lncRNAs (11 downregulated and 4 upregulated) in the PPRV infected spleen samples and 16 lncRNAs (13 downregulated and 3 upregulated) in PPRV infected lung samples were differentially expressed as compared to control. The differentially expressed lncRNAs (DElncRNAs) possibly regulate various immunological processes related to natural killer cell activation, antigen processing and presentation, and B cell activity, by regulating the expression of mRNAs through the cis- or trans-regulatory mechanism. Functional enrichment analysis of differentially expressed mRNAs (DEmRNAs) revealed enrichment of immune pathways and biological processes in concordance with the pathways in which correlated lncRNA-neighboring genes were enriched. The results suggest that a coordinated immune response is raised in both lung and spleen tissues of the goat through mRNA-lncRNA crosstalk.

ACC deaminase and antioxidant enzymes producing halophilic Enterobacter sp. PR14 promotes the growth of rice and millets under salinity stress.

Rhizobacteria are known to ameliorate salinity stress through a wide variety of mechanisms including the production of aminocyclopropane-1-carboxylate deaminase (ACCD). Application of ACCD positive halophilic rhizobacteria ameliorate soil salinity along with its plant growth promotion activity. An effect of the inoculation of ACCD and antioxidant positive and halophilic Enterobacter sp. PR14 was reported on the seed germination and growth of rice and millet seedlings grown in saline and alkaline soil was evaluated. The rhizobacterial strain grew well over a high level of NaCl (15-90 M); at a wide range of pH (5-9); and produced a wide variety of plant growth-promoting (PGP) traits viz. indole-acetic acid (13 µg mL-1), ACCD (5.20 M mg-1 h-1), phosphate solubilization (0.99 g mL-1) and antioxidant enzymes such as superoxide dismutase (5.143 IU mg-1 protein), catalase (0.43 IU mg-1 protein) and glutathione (19.077 µg mg-1 protein) during log phase (30 h) of its growth. The stress with alkaline pH (9) and high salinity (90 M) caused a further increase in the synthesis of PGP traits, ACCD, and antioxidant enzymes. The combined application of Enterobacter sp. PR14, ammonium sulfate (as a substitute of ACC), and NaCl (30 M) resulted in a further increase in the seed germination and vigor in rice and millets vis-à-vis control and other treatments. After 15 days of growth, 61.72% more seed germination in rice and millet and 63.15% increase in sorghum was recorded over the control, and after 30 days of growth, 99.67%, 30%, and 54%, root length 50%, 30% and 54% shoot length in rice, sorghum and millet were observed respectively. A significant increase of 38.13%, 30.75%, and 16.36% in dry weight of rice, sorghum, and millet shoots was recorded respectively. Enterobacter sp PR 14, showing multiple plant growth-promoting traits has a great potential to be used as an efficient bioinoculant for growth promotion of rice and millets under alkaline and saline conditions.

Advances in nanomaterials induced biohydrogen production using waste biomass.

Recent advances on biohydrogen production using different types of waste biomass with the implementation of nanomaterials are summarized. Inspired by exceptional physicochemical and catalytic properties of nanomaterials, the present review focuses on several approaches including impact of nanomaterials on cellulosic biohydrogen production, possible pretreatment technology, as well as improved enzyme & sugar production in order to enhance the biohydrogen yield. Particularly, impacts of nanomaterial are elaborated in detail on different pathways of biohydrogen production (e.g. dark fermentation, photo-fermentation and hybrid-fermentation) using variety of waste biomass. Additionally, emphases are made on the feasibility of nanomaterials for making the biohydrogen production process more economical and sustainable and hence to develop advanced techniques for biohydrogen production using waste biomass.

Bioprospecting microalgae from natural algal bloom for sustainable biomass and biodiesel production.

Natural algal bloom consists of promising algal species which could be a feasible option for the source of bulk biomass and biodiesel production. It has been found in five natural fresh water algal blooms (Uttar Pradesh, India), containing high nitrogen (N) (4.6 ± 0.32 mg/L) and phosphorus (P) (4.12 ± 0.29 mg/L) concentration during spring (23.9-25.9 °C) and summer season (32.0-35.0 °C). Among the isolated algae from naturally occurring bloom, Chlorella sorokiniana MKP01 exhibited highest biomass (1.02 ± 0.02 g/L) and lipid content (174.1 ± 9.6 mg/L) in untreated tap water and urea/single super phosphate (SSP) in the ratio (2:1). The biodiesel quality was assessed and found to be with the Indian and international standards. Algal bloom was artificially developed in the open pond containing 10,000 l tap water supplemented with Urea/SSP (2:1) for a consistent supply of bulk biomass, yielded 8 kg of total biomass and lipid 1.3 kg.

Nanoengineered cellulosic biohydrogen production via dark fermentation: A novel approach.

The insights of nanotechnology for cellulosic biohydrogen production through dark fermentation are reviewed. Lignocellulosic biomass to sugar generation is a complex process and covers the most expensive part of cellulose to sugar production technology. In this context, the impacts of nanomaterial on lignocellulosic biomass to biohydrogen production process have been reviewed. In addition, the feasibility of nanomaterials for implementation in each step of the cellulosic biohydrogen production is discussed for economic viability of the process. Numerous aspects such as possible replacement of chemical pretreatment method using nanostructured materials, use of immobilized enzyme for a fast rate of reaction and its reusability along with long viability of microbial cells and hydrogenase enzyme for improving the productivity are the highlights of this review. It is found that various types of nanostructured materials e.g. metallic nanoparticles (Fe°, Ni, Cu, Au, Pd, Au), metal oxide nanoparticles (Fe2O3, F3O4, NiCo2O4, CuO, NiO, CoO, ZnO), nanocomposites (Si@CoFe2O4, Fe3O4/alginate) and graphene-based nanomaterials can influence different parameters of the process and therefore may perhaps be utilized for cellulosic biohydrogen production. The emphasis has been given on the cost issue and synthesis sustainability of nanomaterials for making the biohydrogen technology cost effective. Finally, recent advancements and feasibility of nanomaterials as the potential solution for improved cellulose conversion to the biohydrogen production process have been discussed, and this is likely to assist in developing an efficient, economical and sustainable biohydrogen production technology.

A novel strategy to enhance biohydrogen production using graphene oxide treated thermostable crude cellulase and sugarcane bagasse hydrolyzate under co-culture system.

Graphene oxide (GO) treated thermostable crude cellulase has been obtained via fungal co-cultivation of strain Cladosporium cladosporioides NS2 and Emericella variecolor NS3 using mix substrate of orange peel and rice straw under solid state fermentation (SSF). Enzyme activity of 60 IU/gds FP, 300 IU/gds EG and 400 IU/gds BGL are recorded in the presence of 1.0% GO in 96 h. This crude enzyme showed 50 °C as optimum incubation temperature, thermally stable at 55 °C for 600 min and stability in the pH range 4.5-8.0. Further, 70.04 g/L of sugar hydrolyzate is obtained from enzymatic conversion of 3.0% alkali pre-treated baggase using GO treated crude cellulase. Finally, 2870 ml/L cumulative biohydrogen production having bacterial biomass ∼2.2 g/L and the complimentary initial pH 7.0 is recorded from sugar hydrolyzate via dark fermentation using co-culture of Clostridium pasteurianum (MTCC116) and a newly isolated Bacillus subtilis PF_1.

Recent development on sustainable biodiesel production using sewage sludge.

Biodiesel as a renewable energy is an important alternative to biofuels in current scenario to explore green energy sources. It is well known that the major cost involved in biodiesel production technology is dependent upon the used feedstock. This review presents an overview of biodiesel production using municipal sewage sludge as a cost-effective substrate. Municipal sewage sludge which possesses high lipid content with zero cost availability can meet the characteristics of a potential feedstock to produce biodiesel. Different types of substrates based processes to produce biodiesel have been also explored in brief. In addition, limitations of the existing process technology for biodiesel production with sustainable solutions have been also discussed.

Overcoming antibiotic resistance: Is siderophore Trojan horse conjugation an answer to evolving resistance in microbial pathogens?

Comparative study of siderophore biosynthesis pathway in pathogens provides potential targets for antibiotics and host drug delivery as a part of computationally feasible microbial therapy. Iron acquisition using siderophore models is an essential and well established model in all microorganisms and microbial infections a known to cause great havoc to both plant and animal. Rapid development of antibiotic resistance in bacterial as well as fungal pathogens has drawn us at a verge where one has to get rid of the traditional way of obstructing pathogen using single or multiple antibiotic/chemical inhibitors or drugs. 'Trojan horse' strategy is an answer to this imperative call where antibiotic are by far sneaked into the pathogenic cell via the siderophore receptors at cell and outer membrane. This antibiotic once gets inside, generates a 'black hole' scenario within the opportunistic pathogens via iron scarcity. For pathogens whose siderophore are not compatible to smuggle drug due to their complex conformation and stiff valence bonds, there is another approach. By means of the siderophore biosynthesis pathways, potential targets for inhibition of these siderophores in pathogenic bacteria could be achieved and thus control pathogenic virulence. Method to design artificial exogenous siderophores for pathogens that would compete and succeed the battle of intake is also covered with this review. These manipulated siderophore would enter pathogenic cell like any other siderophore but will not disperse iron due to which iron inadequacy and hence pathogens control be accomplished. The aim of this review is to offer strategies to overcome the microbial infections/pathogens using siderophore.

Production and Optimization of Physicochemical Parameters of Cellulase Using Untreated Orange Waste by Newly Isolated Emericella variecolor NS3.

Cellulase enzymes have versatile industrial applications. This study was directed towards the isolation, production, and characterization of cellulase enzyme system. Among the five isolated fungal cultures, Emericella variecolor NS3 showed maximum cellulase production using untreated orange peel waste as substrate using solid-state fermentation (SSF). Maximum enzyme production of 31 IU/gds (per gram of dry substrate) was noticed at 6.0 g concentration of orange peel. Further, 50 °C was recorded as the optimum temperature for cellulase activity and the thermal stability for 240 min was observed at this temperature. In addition, the crude enzyme was stable at pH 5.0 and held its complete relative activity in presence of Mn2+ and Fe3+. This study explored the production of crude enzyme system using biological waste with future potential for research and industrial applications.

Efficient dark fermentative hydrogen production from enzyme hydrolyzed rice straw by Clostridium pasteurianum (MTCC116).

In the present work, production of hydrogen via dark fermentation has been carried out using the hydrolyzed rice straw and Clostridium pasteurianum (MTCC116). The hydrolysis reaction of 1.0% alkali pretreated rice straw was performed at 70°C and 10% substrate loading via Fe3O4/Alginate nanocomposite (Fe3O4/Alginate NCs) treated thermostable crude cellulase enzyme following the previously established method. It is noticed that under the optimized conditions, at 70°C the Fe3O4/Alginate NCs treated cellulase has produced around 54.18g/L sugars as the rice straw hydrolyzate. Moreover, the efficiency of the process illustrates that using this hydrolyzate, Clostridium pasteurianum (MTCC116) could produce cumulative hydrogen of 2580ml/L in 144h with the maximum production rate of 23.96ml/L/h in 96h. In addition, maximum dry bacterial biomass of 1.02g/L and 1.51g/L was recorded after 96h and 144h, respectively with corresponding initial pH of 6.6 and 3.8, suggesting higher hydrogen production.

Evaluation of fatty acid profile and biodiesel properties of microalga Scenedesmus abundans under the influence of phosphorus, pH and light intensities.

The present study dealt with biomass, lipid concentration, fatty acid profile and biodiesel properties of microalga Scenedesmus abundans under different phosphate concentrations, pH and light intensities, one at a time. Among different phosphate concentrations, higher biomass (770.10±11.0mg/L) and lipid concentration (176.87±4.6mg/L) were at the concentration of 60mg/L. Light intensity at 6000lux yielded higher biomass and lipid concentration of 742.0±9.7 and 243.15±9.1mg/L, respectively. The biomass (769.0±12.3mg/L) and lipid (179.47±5.5mg/L) concentration were highest at pH 8 and pH 6, respectively. All the culture treatments showed marked effect on the fatty acid profile and biodiesel properties of the extracted oil. FAME derived biodiesel properties were compared with European biodiesel standards (EN 14214), Indian biodiesel standards (IS 15607) and American biodiesel standards (ASTM D 6751-08) to assess the suitability of algal oil as biodiesel feedstock.

Molecular evolution, characterization and expression profiling of uterine aldoketoreductase 1B5 gene in endometrium of goat (Capra hircus).

Aldoketoreductase 1B5 (AKR1B5), a member of the Aldoketoreductase family, is involved in the production of Prostaglandin F2α (PGF2α) as one of vital prostaglandin F synthase (PGFS). PGs (Prostaglandins) play a crucial role in female reproductive system. In the present study, we cloned and characterized the full-length open reading frame of AKR1B5 gene in Black Bengal (BB) goat. The complete coding sequence of AKR1B5 comprises an entire open reading frame of 951 bp, encoding 316 amino acid (AA) residues. BB AKR1B5 showed >82.9% identity with that of cattle, rabbit, human, and rat at nucleotide and amino acid levels, respectively. Further, a systematic study of AKR1B5 sequence evolution was also conducted using Phylogenetic Analysis by Maximum Likelihood (PAML), entropy plot, and Blossum 62 in a phylogenetic context. Analysis of nonsynonymous to synonymous nucleotide substitution rate ratios (Ka/Ks) revealed that negative selection may have been operating on this gene during evolution in goat, cattle, rabbit, human, and rat, which showed its conservation across species. Further, expression of AKR1B5 was determined by quantitative real-time PCR in goat endometrial tissues at different stages of the estrous cycle and early pregnancy. Our results indicated its high expression at luteolytic phase (stage III; day 16-21) during the estrous cycle. However, during early (day ∼30-40) pregnancy the expression was highest as compared to estrous cycle.

Fresh water green microalga Scenedesmus abundans: A potential feedstock for high quality biodiesel production.

Present investigation studied the potential of fresh water green microalga Scenedesmus abundans as a feedstock for biodiesel production. To study the biomass and lipid yield, the culture was grown in BBM, Modified CHU-13 and BG-11 medium. Among the tested nitrogen concentration using Modified CHU-13 medium, the highest biomass and lipid yield of 1.113±0.05g/L and 489±23mg/L respectively was found in the culture medium with 0.32g/L of nitrogen (KNO3). Different lipid extraction as well as transesterification methods were also tested. Fatty acid profile of alga grown in large scale indigenous made photobioreactor has shown abundance of fatty acids with carbon chain length of C16 and C18. Various biodiesel properties such as cetane number, iodine value and saponification value were found to be in accordance with Brazilian National Petroleum Agency (ANP255) and European biodiesel standard EN14214 which makes S. abundans as a potential feedstock for biodiesel production.

Effect of Linum usitatissimum (linseed/flaxseed) fixed oil on experimental esophagitis in albino rats.

INTRODUCTION: The present study was undertaken to elucidate the effect of Linum usitatissimum fixed oil on experimental esophagitis in albino rats. METHODS: Group of rats (n = 6), treated with vehicle control (0.9% NaCl, 3 mL/kg, i.p.) or L. usitatissimum fixed oil (1, 2, 3 mL/kg, i.p.) or omeprazole (30 mg/kg, i.p.). Rats were subjected to pylorus and forestomach ligation to induce esophagitis and were compared to a control sham group. Animals were sacrificed after 6 h and evaluated for the gastric pH, gastric volume, total acidity and esophagitis index. Esophageal tissues were further subjected to estimations of sialic acid, collagen, thiobarbituric acid reactive substances, tissue glutathione, catalase and superoxide dismutase. RESULTS: Treatment with fixed oil significantly inhibited the gastric secretion, total acidity and esophagitis index. The oil also altered the levels of sialic acid and collagen towards normal with significant antioxidant activity in esophageal tissues. CONCLUSION: The lipoxygenase inhibitory, histamine antagonistic, antisecretory (anticholinergic) and antioxidant activity of the oil was attributed for its effect in reflux esophagitis.

Progress of tissue culture and genetic transformation research in pigeon pea [Cajanus cajan (L.) Millsp.].

Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in many developing countries. In India, it is the second important food legume contributing to 80% of the global production. Several biotic and abiotic stresses are posing a big threat to its production and productivity. Attempts to address these problems through conventional breeding methods have met with partial success. This paper reviews the chronological progress made in tissue culture through organogenesis and somatic embryogenesis, including the influence of factors such as genotypes, explant sources, and culture media including the supplementation of plant growth regulators. Comprehensive lists of morphogenetic pathways involved in in vitro regeneration through organogenesis and somatic embryogenesis using different explant tissues of diverse pigeon pea genotypes are presented. Similarly, the establishment of protocols for the production of transgenics via particle bombardment and Agrobacterium-mediated transformation using different explant tissues, Agrobacterium strains, Ti plasmids, and plant selectable markers, as well as their interactions on transformation efficiency have been discussed. Future research thrusts on the use of different promoters and stacking of genes for various biotic and abiotic stresses in pigeon pea are suggested.

Molecular characterization and marker based chemotaxonomic studies of Podophyllum hexandrum Royle.

Detailed chemical studies and RAPD analysis were done in different populations of Podophyllum hexandrum collected from high altitude regions of North Western Himalayas. Random amplified polymorphic DNA (RAPD) analysis revealed a high degree of genetic diversity among the 12 collected accessions, attributed to their geographical and climatic conditions. HPLC analysis also revealed variation in the concentration of two major marker compounds which lead to the identification of a chemotype. The study demonstrated that RAPD and chemical markers are very useful tools to compare the genetic relationship and pattern of variation among such prioritized and endangered medicinal plants.

In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants.

Coleus forskohlii is an endangered multipurpose medicinal plant that has widespread applications. In spite of this, there have been few attempts to propagate its cultivation in India. The present communication presents an in vitro rapid regeneration method using leaf explants of Coleus forskohlii through direct organogenesis. Leaf explants that were excised into three different segments i.e. proximal (P), middle (M) and distal (D) were cultured on Murashige and Skoog (MS) basal medium supplemented with cytokinins. MS Media containing 5.0 mg L(-1) BAP (6-Benzylaminopurine) promoted regeneration of multiple shoots through direct organogenesis from the leaf, which were further elongated on MS media augmented with 0.1 mg L-1 BAP and 0.1 mg L(-1) IAA (Indole-3-acetic acid), cytokinin and auxin combination. Regenerated and elongated shoots, when transferred to ose resulted in profuse rooting plants that were transferred to soil after acclimatization and maintained in a green house. The current protocol offers a direct, mass propagation method bypassing the callus phase of C. forskohlii and is suitable for conservation, large-scale commercial cultivation, and genetic transformation with agronomically desirable traits.

Genetic correlation between chromium resistance and reduction in Bacillus brevis isolated from tannery effluent.

AIMS: To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS: Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic basis of Cr(VI) resistance and reduction, plasmid transfer and curing studies were performed. Among all, B. brevis was resistant to 180 microg Cr(VI) ml(-1) and showed the greatest degree of Cr(VI) reduction (75.8%) within 28 h and its transformant was resistant to 160 microg Cr(VI) ml(-1) and reduced 69.9% chromate. It harboured a stable 18 kb plasmid DNA. Transfer and curing studies revealed that both the chromate resistance and reduction were plasmid mediated. The presence of other metal cations did not have any significant effect on Cr(VI) bioreduction. CONCLUSIONS: Bacillus brevis was resistant to elevated Cr(VI) levels and may potentially reduce it in short time from an environment where other metal ions are also present in addition to chromium ions. The strain tested shows a positive correlation between genetic basis of Cr(VI) resistance and reduction. SIGNIFICANCE AND IMPACT OF THE STUDY: To our knowledge, this is the first study on the genetic correlation between chromium resistance and reduction in bacteria. Such strains may potentially be useful in biotechnological applications and in situ Cr(VI) bioremediation.