Evaluation of aerobic granulation performance bioaugmented with the auto-aggregating bacterium Pseudomonas stutzeri strain XL-2 with heterotrophic nitrification-aerobic denitrification capacity.

In this study, the possibility of an auto-aggregating bacterium Pseudomonas strain XL-2 with heterotrophic nitrification-aerobic denitrification capacity for improving granulation and nitrogen removal was evaluated. The results showed that the supplementation of strain XL-2 promoted granulation, making R1 (experimental group with strain XL-2) dominated by granules at 14 d, which was 12 days earlier than R2 (control group without strain XL-2). This was attributed to the promotion of extracellular polymeric substances (EPS) secretion, particularly proteins by adding strain XL-2, thereby improving the hydrophobicity of sludge and altering the proteins secondary structures to facilitate aggregation. Meanwhile, adding strain XL-2 improved simultaneous nitrification and denitrification efficiency of R1. Microbial community analysis indicated that strain XL-2 successfully proliferated in aerobic granule sludge and might induce the enrichment of genera such as Flavobacterium and Paracoccus that were favorable for EPS secretion and denitrification, jointly promoting granulation and enhancing nitrogen removal efficiency.

Efficient rhodamine B dye degradation by red mud-grapefruit peel biochar catalysts activated persulfate in water.

The continuous and rapid development of textile industry intensifies rhodamine B dye (RhB) wastewater pollution. Meanwhile, massive red mud (RM) solid waste generated by the industrial alumina production process poses detrimental effects to the environment after leaching. For resource utilization and to reduce the expansion of RhB pollution, RM and peel red mud-biochar composite (RMBC) catalyst were synthesized in activating peroxydisulfate (PDS) for RhB degradation. Firstly, characterization results showed that compared to RM, RMBC had a higher content of catalytically active metals (Fe, Al, Ti) (higher than 0.92-4.18%), smaller pore size, and larger specific surface area (10 times), which verified RMBC had more potential catalytic oxidation activity. Secondly, under optimal dosage (catalyst, PDS), pH 4.6, and 20 mg L-1 RhB, it was found that the RhB degradation ratio of RM was 76.70%, which was reduced to 41% after three cycles, while that of RMBC was 89.98% and 67%, respectively. The results indicated that the performance of RMBC was significantly superior to that of RM. Furthermore, the quenching experiments, electron paramagnetic resonance spectroscopy tests, FTIR, and XPS analysis showed the function of O-H, C=O, C-O, Fe-O, and Fe-OH functional groups, which converted the PDS to the active state and hydrolyzed it to produce free radicals ([Formula: see text], 1O2, [Formula: see text]) for RhB degradation. And, Q Exactive Plus MS test obtained that RhB was degraded to CO2, H2O, and intermediate products. This study aimed to raise a new insight to the resource utilization of RM and the control of dye pollution.

Efficient remediation of Mn2+ and NH4+-N in co-contaminated water and soil by Acinetobacter sp. AL-6 synergized with grapefruit peel biochar: Performance and mechanism.

Electrolysis manganese slag produced in industrial manganese production causes massive leachate containing heavy metal Mn2+ and inorganic NH4+-N, which causes serious hazard to the water body and soil. A cost-effective alternative to address the multiple pollution is urgently needed. This study investigated the synergy of grapefruit peel biochar (BC) and strain AL-6 to remediate Mn2+ and NH4+-N in sequencing batch bioreactor (SBR) and soil column. The results showed that, in SBR, under the condition of C/N 5, temperature 30°C, BC and strain AL-6 showed fabulous performance to remove Mn2+ (99.3%) and NH4+-N (97.7%). The coexisting ions Mg2+ and Ca2+ had no effects on the removal of Mn2+ and COD, however, 23.3% removal efficiency of NH4+-N was curtailed. Characterization found that the presence of MnCO3 confirmed the adsorption of Mn2+ by functional groups action, and gas chromatography indicated that BC and strain AL-6 promoted the reduction of N2O and organic carbon. In addition, BC and strain AL-6 helped to immobilize 799.41 mg L-1 of Mn2+ and 320 mg L-1 of NH4+-N after 45 d in the soil column. And the determination of TOC, CEC, pH, Eh, soil enzymatic activity (catalase and urease), and microbial diversity and abundance confirmed that BC and strain AL-6 increased the soil fertility and bioavailability of pollutants. Totally, BC and strain AL-6 possess great potential to remediate Mn2+ and NH4+-N pollution in water and soil.

Ni(II), Cr(VI), Cu(II) and nitrate removal by the co-system of Pseudomonas hibiscicola strain L1 immobilized on peanut shell biochar.

At present, the improvement of nitrate and mixed heavy metals removal in wastewater by microorganism are urgently needed. Previous studies have shown that Pseudomonas hibiscicola strain L1 exhibited Ni(II) removal ability under aerobic denitrification. In this study, the characteristics of the free strain L1, peanut shell biochar (PBC) and further the co-system of strain L1 immobilized on PBC were investigated for the removal of Ni(II), Cr(VI), Cu(II) and nitrate in mix-wastewater. The results illustrated that strain L1 could remove 15.51% - 32.55% of Ni(II) (20-100 mg·L-1), and removal ratios by co-system were ranked as Ni(II) (81.17%) > Cu(II) (45.84%) > Cr(VI) (38.21%). Scanning Electron Microscope (SEM), X-ray Diffractometer (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) images indicated that the strain L1 immobilized well on PBC and had vigorous biological activity; the crystals of Ni(OH)2, Cu(OH)2 and CrO(OH) etc. were formed on surface of co-system with various functional groups participated in. In Sequential Batch Reactor (SBR), the pollutant removal ratios by co-system were higher than that by free strain L1. This study illustrated that the co-system of strain L1 immobilized on PBC was qualified to be applied for practical scenarios of effective heavy metal removal of electroplating mix-wastewater.