ABSTRACT
We studied in mice the neuropharmacological effect of some 5-substituted bromopyrazolinic spirobarbiturates. LD50 and CL50 values were estimated for all the investigated compounds. The effect on potentiation of sodium pentobarbital activity and on generalised tonic-clonic seizures produced by PTZ on mice was studied. All the compounds had the same pharmacological profile, they extended sleeping-time induced by sodium pentobarbital and showed protection against PTZ-induced convulsions, decreasing the death rate.
Subject(s)
Anticonvulsants/chemical synthesis , Anticonvulsants/pharmacology , Hypnotics and Sedatives/chemical synthesis , Hypnotics and Sedatives/pharmacology , Pyrazoles/chemical synthesis , Pyrazoles/pharmacology , Animals , Anticonvulsants/toxicity , Behavior, Animal/drug effects , Convulsants/pharmacology , Drug Synergism , Hypnotics and Sedatives/toxicity , Injections, Intraperitoneal , Male , Mice , Pentobarbital/pharmacology , Pentylenetetrazole/antagonists & inhibitors , Pyrazoles/toxicity , Rats , Rats, Wistar , Seizures/chemically induced , Seizures/prevention & control , StereoisomerismABSTRACT
A methanolic extract of Hypericum empetrifolium Willd. was evaluated for anti-inflammatory properties in rats (subplantar edema induced by carrageenan) and analgesic effects in mice (hot plate and writhing tests). Our results showed that the methanolic extract exhibits a significant anti-inflammatory activity and analgesic effects only in one of the experimental models (writhing test). Therefore, we may suppose that the methanolic extract of H. empetrifolium is active against inflammatory pain.