ABSTRACT
Aim: To evaluate the cleaning ability of two single-file rotary systems- Self-Adjusting File (SAF) and Wave one (WO) systems in removing the smear layer using sodium hypochlorite (NaOCl) + ethylene diamine tetraacetic acid (EDTA) and NaOCl + Qmix as irrigants at apical one-third of the root canal. Methods: Forty extracted human mandibular premolars were selected and working length was determined. The canal was manually instrumented up to a number 25 size K-file. The roots were divided into the following groups with 10 samples each - Group 1 using SAF: Group 1a- 3% NaOCl + 17% EDTA, Group 1b- 3% NaOCl + Qmix. Group2 using WO: Group 2a- 3% NaOCl + 17% EDTA, Group 2b- 3% NaOCl + Qmix. In the SAF group, the irrigation was performed continuously using the special irrigation apparatus. In the WO group, syringe irrigation was done followed by final irrigant activation using passive ultrasonic irrigation (PUI). The roots were sectioned longitudinally and subjected to scanning electron microscopic (SEM) examination. The amount of smear layer was evaluated using a five score index at the apical third level. Statistical analysis was performed using the Chi-square test. Results: Group 1 (SAF) showed better canal cleanliness at apical third compared to Group 2 (WO) with both irrigant combinations and the results were statistically significant (p<0.05). 3% NaOCl + Qmix was equally as effective as 3% NaOCl + 17% EDTA in removing the smear layer with no significant difference between them. Conclusion: Within the limitation of this study, SAF in combination with 3% NaOCl + Qmix or 3% NaOCl + 17% EDTA should be used for removing smear layer in critical areas of the root canal.
ABSTRACT
AIM: The aim of this study was to compare the osteogenic potential of conventional glass-ionomer cement (GIC) with chitosan-modified GIC (CH-GIC) and bioactive glass-modified GIC (BAG-GIC) as a function of time in varying proportions. MATERIALS AND METHODS: CH-GIC was prepared by adding 10 v/v% (Group II) and 50 v/v% (Group III) CH to the commercial liquid of GIC. BAG-GIC was prepared by the addition of 10 wt% (Group IV) and 30 wt% (Group V) of BAG to the GIC powder. Conventional GIC was kept as Group I. Nine round-shaped samples measuring 2 mm thick and 5 mm in diameter were prepared for every experimental material. Human osteosarcoma cells were cultured and cell proliferation was assessed at 24, 48, and 72 h using 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay, and cell differentiation was assessed at 7,14, and 21 days using alkaline phosphatase (ALP) assay. All experiments were done in triplicate. The data obtained were analyzed using one-way analysis of variance and Tukey honestly significant difference post hoc multiple comparisons at 0.05 level significance. RESULTS: Cell culture studies showed a significant increase in proliferative activity and ALP activity in Group II, III, IV, and V than Group I at all-time intervals (P < 0.05). There was no statistically significant difference in osteogenic potential between CH-GIC and BAG-GIC groups. CONCLUSION: The osteogenic potential was significantly higher in CH-GIC and BAG-GIC compared to conventional GIC.
ABSTRACT
AIM: This study aims to compare the cleaning efficacy of root canal walls using two rotary single file systems: self-adjusting file (SAF) and WaveOne (WO). MATERIALS AND METHODS: Forty extracted human premolars with similar range of canal curvature were selected, decoronated, working length determined and roots were divided into two groups of 20 samples each: Group I - SAF and Group II - WO. All root canals were irrigated with 3% sodium hypochlorite (NaOCl) and 17% ethylenediaminetetraacetic acid (EDTA). The roots were sectioned longitudinally and subjected to scanning electron microscopic examination. The amount of debris and smear layer was evaluated using five score index at coronal, middle and apical third levels. Statistical analysis was performed using the Chi-square test and significance was set at a P < 0.05. RESULTS: Statistically significant difference was observed between the groups in cleaning the apical third. Group I (SAF) showed better canal cleanliness compared to Group II (WO) in the apical third. CONCLUSION: Within the limitations of this study, SAF in combination with 3% NaOCl and 17% EDTA irrigating solution had significantly better cleaning efficacy in the apical third of root canals when compared to WO rotary file system.
ABSTRACT
This case report describes endodontic treatment completed in a peg-shaped maxillary lateral incisor, with single root and five root canals of which, one is due to dens invaginatus. Cone beam computed tomogram scanning confirmed the unique morphology of the tooth. New nomenclature for the five canals is proposed.
