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Biotechniques ; 73(1): 18-24, 2022 06.
Article in English | MEDLINE | ID: mdl-35698846

ABSTRACT

Highly sensitive diagnostic tools are crucial for individual screening during an epidemic of leptospirosis. To aid in developing a diagnostic tool for the sensitive detection of pathogenic strains, a new approach targeting nucleic acid amplification that combines quantitative PCR (qPCR) and strand displacement isothermal amplification was evaluated. The effectiveness of the combined approach, a quantitative polymerase chain displacement reaction (qPCDR), was compared with a qPCR technique. The results showed that qPCDR presented higher sensitivity (at least tenfold) and shorter reaction time than the qPCR approach for pathogenic Leptospira spp. detection. Thus, the qPCDR-based technique developed in this study is a promising approach for pathogenic Leptospira spp. detection and the further development of a diagnostic kit.


Subject(s)
Leptospira , Leptospirosis , Nucleic Acids , Humans , Leptospira/genetics , Leptospirosis/diagnosis , Real-Time Polymerase Chain Reaction/methods , Sensitivity and Specificity
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