Selected bacteria in sheep stool depending on breed and physiology state.

One of the important factors influencing the microbial community of ruminants, besides environment or diet, are breed and physiology. Therefore, the purpose of this study was to assess these changes in the levels of basic microbial phyla and families. For this study, qPCR analysis was performed to determine the level of bacteria (Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria clusters and Clostridiaceae, Lactobacillaceae families) in the feces of ewes of three native Polish sheep breeds (Polish Lowland Sheep (PON), Swiniarka Sheep (SW), and synthetic line BCP) at different physiological periods (conception, early pregnancy, lambing, end of lactation). The animals were kept in the same environment and were at the same age (2-years). The results showed a significant effect of both breed (p = 0.038) and physiological period (p < 0.05, p < 0.01) on the levels of bacteria analyzed. The breed showed differences across physiological periods. The influence of the race factor was noted primarily between the BCP synthetic line and the other two breeds (differences in terms of all analyzed clusters and families except Actinobacteria phyla). In the case of SW and PON, however, the observed differences were only at the level of Proteobacteria cluster and Clostridiaceae family. On the other hand, the early pregnant and lambing periods were the most microbiologically diverse in terms of the analyzed clusters and families of bacteria.

Influence of selected factors on the Firmicutes, Bacteroidetes phyla and the Lactobacillaceae family in the digestive tract of sheep.

In this study, we used 10 healthy sheep, which gave birth to healthy twins. Stool samples were collected from mothers and their offspring 3 times during the study (0, 28 and 56 day postpartum). Milk samples were taken from the mothers at the same time. RT PCR analysis of faeces and milk was performed in order to assess the level of bacteria from the Firmicutes and Bacteroidetes phyla including the family Lactobacillaceae (phylum Firmicutes). The composition of mother's milk was also analyzed and their BCS. The data were compiled statistically. The obtained results showed that the level of the studied groups of bacteria may change due to the change of diet. Additionally, there were significant differences between lambs and mothers in the levels of the studied groups of bacteria. Analysis also shown that in the digestive system of mothers was a smaller disproportion in the level of the studied bacterial phyla than in lambs. The results also indicated the occurrence of differences in the bacterial composition at the individual level, both in ewes and their offspring. Additionally, in the conducted experiment, there were differences in the level of Firmicutes and Bacteroidetes groups depending on the sex.

Variation in Wool Characteristics across the Body in a Herd of Alpacas Kept in Poland.

Wool characteristics vary depending on where on the body the wool is produced. Knowledge of this variation is important in order to separate the fleece into more homogenous parts. Similar parts from different animals can then be pooled to create batches of wool with similar characteristics. This will allow for better alpaca products with less variation. The aim of this study was to determine the variation in fiber diameter, medullation, and staple length across the body of alpacas from one herd. Wool samples were collected from 16 adult (3-5 years old) females: from the saddle (S), midside (MS), neck (N), and pieces (P). The mean fiber diameter (MFD) and medullation were measured using a projection microscope according to the IWTO-8-2011 standard. The fiber diameter of the pieces was greater (p < 0.05) than for the S, MS, and N areas. The highest medullation was found on the neck. The neck was also characterized by the shortest wool. The MFD for the fleece, excluding P, showed the strongest correlation (r = 0.927) with the MFD of MS. The study showed that due to the variation of fiber diameter, the incidence of medullation, and staple length, the wool from the pieces and the neck should be separated from the total fleece. The most appropriate site from where to collect a representative sample of wool from the fleece for testing purposes is the midside site of the animal.

Evaluation of Changes in the Levels of Firmicutes and Bacteroidetes Phyla of Sheep Feces Depending on the Breed.

Studies carried out so far have indicated the effect of the microbiome on the composition of ruminant products. Recent studies have shown that not only diet, but also genetic factors can affect the microbiological composition of the digestive system. The aim of the study was to determine the differences in the levels of selected bacterial phyla in terms of breed differences. Three sheep breeds, i.e., Olkuska, Romanov, and old-type Polish Merino, differing in their use (meat-wool, meat, prolificacy) and country of breed origin were included in the study. Sheep at the same age and of the same sex were kept for a period of 3 months in the same environmental conditions and fed the same feed in the same proportions. The study included real-time PCR (polymerase chain reaction) analysis of feces collected before the slaughter and measurements of body weight and chilled carcasses. The obtained results showed significant differences between the breeds in the levels of bacterial populations tested. There were also differences in body weight between the breeds during the first weight measurements, however, the final results did not show any differences-after three months of maintenance all of them reached similar body weights, despite differences in fecal microbiological composition. The study suggests that in addition to diet and environmental conditions, the microbiology can also be influenced by breed.

The effect of genotype and muscle type on the physico-chemical characteristics and taurine, carnosine and L-carnitine concentration in lamb meat.

An experiment was conducted to determine the chosen bioactive components and physico-chemical characteristics of lamb meat of different animal genotypes and the muscle types. The 22 ram lambs of Polish Merino (PM) and 22 crossbreeds of Polish Merino  × â€¯Berrichone du Cher (PMB) were fattened to achieve their slaughter weight of 40 kg. After slaughter, the carcasses were kept at 4  ∘ C for 24 h. Then, the samples of longissimus lumborum (LL) and gluteus medius (GM) muscle were collected to analyse the physico-chemical traits; fatty acid profile; and concentrations of taurine, carnosine, L-carnitine. The GM muscle compared to LL had the higher value ( P  < 0.05) of L * and a lower value ( P  < 0.05) of b * and H * both in PM and PMB lambs. The value of expressed juice was lower ( P  < 0.05) in both LL and GM muscles of PM lambs. A higher amount ( P  < 0.05) of collagen was found in LL muscle compared to GM both in PM and PMB lambs. The GM muscle of PM lambs showed higher ( P  < 0.05) conjugated linoleic acid (CLA) content, as well as higher total polyunsaturated acids (PUFAs), PUFA n-6, and PUFA n-3 ( P  < 0.05). The GM muscle was characterized by a higher ( P  < 0.05) content of taurine, while in the LL muscle there was a higher amount ( P  < 0.05) of carnosine. A larger amount ( P  < 0.05) of L-carnitine was found in GM muscle but only within PMB lambs. The obtained results showed a greater impact of the lamb's genotype on the physical characteristics of meat than on its chemical composition and the content of bioactive components. The muscle type had an effect on meat colour; collagen content; fatty acid profile; and amount of taurine, carnosine, and L-carnitine present.

The effect of aging and muscle type on the quality characteristics and lipid oxidation of lamb meat.

The research carried out on meat from 45 ram lambs of the Polish merino breed allowed to determine the effect of meat aging and muscle type on physicochemical characteristics and oxidative stability of lipids. Analysis of physicochemical traits (pH, meat color, expressed juice, cooking loss, shear force, moisture, protein, fat and total collagen content) was performed on fresh and meat aged for 14 d in the longissimus lumborum (LL) and gluteus medius (GM) muscles. The meat aging determined all physicochemical characteristics except protein and fat content. More changes in pH and meat color parameters were defined in the GM muscle compared to the LL muscle. The increase in the tenderness of meat expressed as a reduction ( P < 0.05 ) of shear force values was observed in both muscles aged for 14 d. A lower value ( P < 0.05 ) of the shear force, despite the higher content of collagen, was determined in the GM muscle compared to LL. The investigated muscles differed in the degree of lipid peroxidation expressed as thiobarbituric acid-reactive substances (TBARS) in both fresh and aged meat. The TBARS value was lower ( P < 0.05 ) in the LL muscle than in GM. In the longissimus lumborum muscle, the significantly lower content of polyunsaturated fatty acids (PUFAs) and PUFA n-6 has been recorded. The oxidation stability was not influenced by the meat aging.

The fatty acid profile of muscle tissue of ram lambs with diverse genotypes.

The aim of the study was to determine the fatty acid profile of intramuscular fat for genetically diverse sheep breeds kept in the same environmental and feeding conditions. The study was conducted on 30 (15 in each breed) wrzosówka and zelaznenska ram lambs slaughtered at 23-25 kg of life weight. The meat samples for analysis were taken from longissimus lumborum muscle. The meat of wrzosówka ram lambs contained almost twofold less (P < or = 0.01) intramuscular fat compared to zelaznenska sheep. Lower (P < or = 0.05) total of SFA and higher (P < or = 0.05) MUFA content in muscle tissue of wrzos6wka sheep was shown. The predominant monounsaturated acid was oleic acid which amounted to almost 90% of all MUFA. The activity of enzyme Delta9 desaturase evidenced by the higher values of C14:1/C14:0, C18:1/Cl8:0 (P < or = 0.01) and C16:1/C16:0 (P < or = 0.05) indices was also recorded for wrzos6wka ram lambs. The UFA/SFA (P < or = 0.05) and MUFA/SFA (P < or = 0.05) ratios as well as the value of the trombogenic index (TI) (P < or = 0.05) were more favourable in muscle tissue for wrzos6wka than zelaznenska rams lambs. Therefore, the meat from leaner animals is more beneficial for human health.

The content of conjugated linoleic acid (CLA) isomer groups in milk of two Polish sheep breeds determined by silver ion liquid chromatography (Ag(+)-HPLC).

Sheep milk is rich in CLA isomers which are biologically active components influencing human health. There are four geometric CLA isomer pairs: cis, trans; trans, cis; trans, trans and cis, cis. The aim of the present study was the analysis of CLA isomer groups content by Ag+-HPLC in milk fat of Zelaznenska (ZS) and Wrzosówka (WS) sheep breeds. The ewes of both breeds were kept under the same environmental and nutritional conditions. Milk samples were collected from 60 suckling ewes (30 from each breed), at the age of 3-4 years and in their 4th week of lactation. A higher total amount of all CLA isomer groups was obtained in milk of ZS ewes, however, this result was statistically insignificant. The percentage of the main c9, t11 isomer in total CLA was higher in Wrzosówka milk (68% vs. 74%). The content of the trans, trans isomer group in milk fat of the studied breeds was similar. The percentage of this group in total CLA in milk of WS and ZS constitutes 7.2% and 7.7%, respectively. The amount of cis,cis isomers in milk fat of ZS was higher thanWS (P < or = 0.01).

The Ewe's Reproductive Performance, Growth Rate and Carcass Quality of Lambs Kept in a Barn vs Those Kept under an Overhead Shelter.

A HERD OF POLISH LOWLAND LOCAL SHEEP WAS DIVIDED INTO TWO EQUAL GROUPS: the first group was kept under an overhead shelter, and the second group was kept in a warm barn. The effect of maintenance on ewe's reproductive performance, survival as well as the growth rate of lambs, and their carcasses quality was investigated. The lack of differences in fertility and prolificacy of ewes as well as in the survival and growth rate between the groups confirmed a good adaptation of Zelaznienska sheep to low temperature. Harsh environmental conditions did not cause a significant decrease of the body weight growth; however, they brought in an (insignificant) reduction of subcutaneous fat thickness and meatiness of the loin part of a lamb's body. The fat content of carcasses obtained from lambs reared under the overhead shelter was significantly lower, with no differences of meat and bones contribution between the groups.