ABSTRACT
BACKGROUND: The finding of new biomarkers is needed to have a better sub-classification of primary renal tumors (RCC) as well as more reliable predictors of outcome and therapy response. In this study, we evaluated the role of circulating FGF21, an endocrine factor, as a diagnostic and prognostic biomarker for ccRCC. MATERIALS AND METHODS: Serum samples from healthy controls (HC), clear cell and chromophobe RCC cancer patients were obtained from the serum biobank "Biobanco Público de Muestras Séricas Oncológicas" (BPMSO) of the "Instituto de Oncología "Ángel H. Roffo". Serum FGF21 and leptin were measured by ELISA while other metabolic markers were measured following routinely clinical procedures. RESULTS: One of our major findings was that FGF21 levels were significantly increased in ccRCC patients compared with HC. Moreover, we showed an association between the increased serum FGF21 levels and the shorter disease free survival in a cohort of 98 ccRCC patients, after adjustment for other predictors of outcome. CONCLUSION: Our results suggest that higher FGF21 serum level is an independent prognostic biomarker, associated with worse free-disease survival.
Subject(s)
Humans , Male , Adult , Female , Middle Aged , Aged , Central Nervous System Neoplasms/diagnosis , Glioma , Astrocytoma , Glioblastoma , Biomarkers, Tumor , Gene Expression , Survival Analysis , Awards and Prizes , Retrospective Studies , Disease Progression , Prognosis , Genes, DCC , Molecular Biology , Receptors, Platelet-Derived Growth Factor , Receptors, Growth Factor , Cyclin-Dependent Kinase Inhibitor p16 , Ki-67 Antigen , Genes, bcl-2 , Tumor Suppressor Protein p53Subject(s)
Humans , Male , Adult , Female , Middle Aged , Astrocytoma , Central Nervous System Neoplasms , Gene Expression , Glioblastoma , Glioma , Biomarkers, Tumor , Survival Analysis , Awards and Prizes , Cyclin-Dependent Kinase Inhibitor p16 , Genes, bcl-2 , Genes, DCC , Molecular Biology , Prognosis , Disease Progression , Receptors, Growth Factor , Receptors, Platelet-Derived Growth Factor , Retrospective Studies , Tumor Suppressor Protein p53ABSTRACT
LP07 is a new cell line derived from P07 lung tumor, spontaneously arisen in a BALB/c mouse. LP07 is composed of heterogeneous epithelioid polyhedric cells that proliferate at a slow rate, have low plating efficiency and are unable to grow in soft agar. Only some LP07 cells expressed cytokeratins while most of them were positive for vimentin. Ultrastructure studies showed that LP07 cells established rudimentary intercellular unions, formed glandular-like conducts and presented conspicuous secretory granules, suggesting an epithelial-glandular origin, with neuroendocrine components. Upon injection LP07 cells formed poorly differentiated non-invasive adenocarcinomas, and tumor bearing mice developed leukocytosis, hypercalcemia and cachexia. This tumor cell line constitutes a useful tool to study lung tumor biology and paraneoplastic syndromes.
