Effects of microwave vacuum drying and conventional drying methods on the physicochemical and microstructural properties of squid shreds.

BACKGROUND: Microwave vacuum drying is an innovative technology for drying fruits and vegetables. However, this technology has not been well explored for drying seafood. In the present study, the effects of microwave vacuum drying (MVD), hot air drying (HAD) and sun drying (SD) on the proximate composition, texture, rehydration, color and microstructure of squid shreds were investigated. RESULTS: Proximate components of the samples dried by different methods were not significantly different, but the drying time was markedly lower for the MVD technique. The rehydration rate and water absorption index followed the order MVD > HAD > SD. The extent of browning as indicated by the b* value was significantly lower in MVD samples (14.38) compared with HAD (19.47) and SD (21.94) samples. MVD resulted in the lowest values for hardness, springiness and chewiness of both dried and rehydrated squid shreds, while SD recorded the highest values for the same. Scanning electron microscopy (SEM) images of the muscle fiber indicated muscle shrinkage and more toughness in SD samples, whereas Fourier transform infrared (FTIR) spectra revealed a small extent of protein degradation in MVD samples. CONCLUSION: The study revealed that higher-quality dried squid shreds can be prepared by microwave vacuum drying in a shorter time compared with the traditional methods of sun drying and hot air drying. Hence microwave vacuum drying can be used as an energy-efficient and time-saving technology to make dried seafood of superior quality. © 2019 Society of Chemical Industry.

Assessment of polyaromatic hydrocarbons and pesticide residues in domestic and imported pangasius (Pangasianodon hypophthalmus) fish in India.

BACKGROUND: The pangasius catfish (Pangasianodon hypophthalmus) is a ubiquitous item of seafood in global markets. However, pesticide residues in aquaculture fish, arising from agricultural run-off and/or direct application during pond preparation, pest control and harvest, are a potential food safety concern. This study assessed the level of chemical hazard in domestic and imported pangasius fish in India. RESULTS: A total of 119 contaminants, including polyaromatic hydrocarbons (PAHs), organochlorines (OCs) and other groups of pesticides, were screened in 148 samples during 2014-2015 as the first endeavour of its kind in an Indian context, employing a validated gas chromatography/tandem mass spectrometry (GC/MS/MS) method. Apart from the low-level incidence of OCs, pesticides such as quinalphos, malathion, parathion-methyl, etc. were detected in 38% of the samples. In comparison with Indian pangasius, fewer contaminants at low residue level were detected in pangasius fillets imported from Vietnam. CONCLUSION: The human dietary exposures of the residue concentrations detected were less than the maximum permissible intakes and hence appeared safe. However, detection of commonly used pesticides indicated their direct application in aquaculture and contamination from agricultural run-off. This emphasizes the need for continual residue monitoring in aquaculture fish. © 2015 Society of Chemical Industry.

Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches.

AIMS: The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequences. METHODS AND RESULTS: Non-O1 and non-O139 V. cholerae isolates were obtained from shrimp pond water, pond sediment, shrimp head and shrimp muscle. RS-PCR yielded fewer bands than REP-PCR and ERIC-PCR. Higher similarity was observed in RS-PCR (75-100%) than in REP-PCR (60-95%) and ERIC-PCR (40-95%). CONCLUSIONS: A 100% similarity between V. cholerae isolates was only noticed in RS-PCR. The choleratoxigenic V. cholerae (non-O1 and non-O139) showed greater genetic similarity with ctx-negative V. cholerae than among ctx-positive V. cholerae. SIGNIFICANCE AND IMPACT OF THE STUDY: The greater similarity of ctx-positive V. cholerae with ctx-negative V. cholerae isolates indicates that the ctx-positive strains (non-O1 and non-O139) might have originated from autochthonous V. cholerae in the aquatic niche.