ABSTRACT
Diabetes mellitus is a severe devastating epidemic has an effect on both developing and developed countries. Diabetic neuropathy (DN) is a secondary microvascular complication of diabetes causing damage to the nerves and is characterized by fall in nerve conduction velocity, severe pain, impaired sensation and degeneration of nerve fibres. In the present study, we investigated the neuroprotective effect of ethanolic extract of Cleome viscosa (EECV) against streptozotocin (STZ) induced diabetic neuropathy in Wistar rats. Intraperitoneal injection of STZ resulted in significant increase in thermal hyperalgesia and hyperlipidemia after four weeks. Antioxidant enzyme [superoxide dismutase (SOD), glutathione (GSH) and catalase) levels were reduced and malondialdehyde (MDA) level was increased significantly in diabetic rats as compared to the vehicle control rats. Four weeks of chronic treatment with EECV (100, 200 and 400 mg/kg) attenuated the level of nociceptive threshold significantly and dose dependently. It also decreased the elevated levels of lipids, lipid peroxidation, and oxidative stress significantly and dose dependently. The present study provides investigational evidence of the protective effect of EECV on nociception; hyperlipidemia and oxidative stress in STZ induced diabetic neuropathy.
Subject(s)
Cleome , Diabetes Mellitus, Experimental/complications , Diabetic Neuropathies/drug therapy , Hyperalgesia/drug therapy , Lipids/blood , Oxidative Stress/drug effects , Phytotherapy , Plant Extracts/therapeutic use , Animals , Catalase/metabolism , Lipid Peroxidation/drug effects , Male , Rats , Rats, Wistar , StreptozocinABSTRACT
The present article is an attempt to validate the in vitro skin corrosion human skin model test as per the OECD test guidelines 431 as an alternative method for in vivo skin corrosion/irritation test. All over India, in vivo skin corrosion/irritation test is commonly used, rather than in vitro skin corrosion models. Hence, the present study was under taken with EpiSkinTM, SNC, Lyon, France to validate this in vitro model. Various corrosive and non corrosive chemicals with their known skin corrosive property were used to validate the study as specified under the guideline (OECD 431). The results obtained in this study were in accordance with the corrosive properties of the respective chemicals.
