Production and Evaluation of Encapsulated Zinc Oxide on Performance, Ileal Digestibility and Zinc Transporter Gene Expression in Broiler Chicken.

The present study was undertaken for the production of encapsulated zinc and its evaluation in broiler chicken diet. The process of microencapsulation involved the use of polymers, gum arabic and maltodextrin with a maximum encapsulation of efficiency of 66%. Encapsulated material contained about 20% zinc oxide (ZnO) as core material following the freeze-drying process. One hundred and ninety-two-day-old broiler chicks were distributed in four groups in six replications having eight birds in each. The four groups comprised control (inorganic source of zinc), En-Zn-100 (encapsulated zinc at 100% of control), En-Zn-50 (encapsulated zinc at 50% of control), and Org-Zn-50 (Zn-methionine at 50% of control). The experiment was carried out for 35 days following standard management practices. The live weight gain, feed intake and FCR were comparable among groups. Plasma and muscle zinc (ppm) content was unaffected by the level or source of zinc supplementation. The zinc apparent ileal digestibility coefficient was significantly (P < 0.05) higher in En-Zn-50 fed groups, while crude protein digestibility was not affected by the level or form of Zn supplementation. Bone weight, length, and zinc content were comparable, and bone ash content was significantly different among the groups. Relative expression of ZnT2 was significantly upregulated in encapsulated zinc-fed groups. From the study, it could be concluded that supplementation of zinc either as encapsulated or organic form at 50% of inorganic source (ZnO) could be sufficient to maintain the growth performance, serum, tissue and bone mineral content in broiler chicken.

Nano zinc supplementation in goat (Capra hircus) ration improves immunity, serum zinc profile and IGF-1 hormones without affecting thyroid hormones.

The trial was aimed at evaluating probable superiority, if any of nano zinc (NZn) over inorganic zinc (Zn) on immunity, serum minerals and T3 , T4 , and IGF-1 hormone profiles in goats. NZn was synthesized by using 0.45 M aqueous solution of Zn nitrate and 0.9 M aqueous solution of sodium hydroxide (average particle size 74 nm). Twenty-four male goats were grouped into four groups as per their body weight and were supplemented with either a basal diet with concentrate and straw at 50:50 ratio (Negative control, NC) alone or supplemented with 50 mg/kg Zn (Control) from inorganic Zn source, that is ZnO (IZn-50), 50 mg/kg Zn from NZn (NZn-50) or 25 mg/kg Zn from NZn (NZn-25). No change was observed in thyroid hormone status on zero and 90th day of experimental feeding, but NZn supplementation improved (p < 0.05) IGF-1 level on 90th day serum samples. Zn supplementation improved the humoral immunity in all the groups irrespective of the source. Similarly, cell-mediated immunity (CMI) measured by skinfold thickness after injecting Con-A, was also improved in Zn supplemented groups than control at 6, 12 and 48 h of incubation. NZn-50 animals showed highest HI (haemagglutination inhibition) titre as well as skin thickness. The CD 4 + (cluster of differentiation in %) was more (p < 0.05) in Zn supplemented groups. NZn-50 showed higher (p < 0.05) CD 8 + count than NC and similar (p > 0.05) to IZn-50 and NZn-25 groups without affecting (p > 0.05) the ratio of CD 4 + , CD 8 + among the treatment groups. Thus, NZn supplementation at 25 mg/kg had similar immunity and serum T3 , T4 and IGF-1 profiles compared with IZn at 50 mg/kg dose.

Effect of Supplementation of Nano Zinc Oxide on Nutrient Retention, Organ and Serum Minerals Profile, and Hepatic Metallothionein Gene Expression in Wister Albino Rats.

A study was conducted to validate the effects of nano form of zinc (NZn) on nutrient digestibility, zinc retention, organ and serum zinc profile, and hepatic metallothionein gene expression in Wistar albino rats (WAR). Nano zinc (NZn) was synthesized through chemical method, by using 0.45 M zinc nitrate [Zn(NO3)2.6H2O] and 0.9 M sodium hydroxide (NaOH). The NZn particle in its oxide form was characterized by TEM-EDAX and XRD, and found to be in nano range (below 100 nm. Zinc was supplemented to the Wistar albino rats (WAR) through synthetic semi-purified diet either without Zn, or as inorganic zinc (IZn; 25 mg/kg), or as synthesized NZn (25, 12.5, 6.25, 3.125 or 50 mg/kg DM) for 60 days. The zinc content was observed to be significantly (P < 0.05) higher in liver, bone, kidney, and serum due to NZn supplementation where NZn-50 had highest zinc content and control had the least, without affecting Fe, Mn, and Cu. NZn at 12.5 mg/kg group rats were either comparable or better than IZn at 25 mg/kg in terms of zinc retention, CP digestibility, zinc level in serum, liver, bone, and kidney suggesting its better bioavailability simultaneously also reduced fecal excretion of zinc to the environment. Metallothionein mRNA expression was upregulated in NZn at 25 mg/kg and NZn at 50 mg/kg than IZn at 50 mg/kg. Thus, in WAR, NZn at half of the ICAR recommendation (25 mg/kg DM) is as effective as inorganic zinc at 100% of recommended dose.

Evaluation of maize grain and polyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, sperm functional parameters and fertility.

The objective of the present study was to elucidate the effect of different sources of dietary energy (maize vs polyunsaturated fatty acid (PUFA) on semen functional parameters and fertility of adult rams. Eighteen adult rams were divided into two groups (maize and PUFA, n=9). The main energy source for the rams in the maize group was coarsely ground maize grain, whereas in the PUFA group it was sunflower oil (rich in 18:2 linoleic acid, an omega-6 acid). The ration was fed for a minimum period of 60 days and thereafter semen was collected for evaluation. The proportion of progressive forward motility was significantly (P<0.05) higher in the PUFA group compared with the maize group. Sperm lipid peroxidation as measured by malondialdehyde formation (µM per 1×10(9) spermatozoa) was significantly (P<0.05) higher in the PUFA group compared with the maize group. When the semen was diluted with Tris-egg yolk-citrate buffer and incubated for 24h at 4°C, the proportions of plasmalemma integrity, the sperm subpopulation positive for functional membrane and acrosomal integrities, and mitochondrial membrane potential were significantly (P<0.05) higher in PUFA-fed than in maize-fed animals. The different sources of energy did not influence the serum and seminal plasma IGF-I levels. The cleavage rate (percentage) did not differ significantly between PUFA- (45.4±4.91) and maize- (44.63±6.8) fed animals. In conclusion, PUFA feeding influenced sperm quality by altering or stabilising membrane integrity. The present study indicates that PUFA may improve semen quality but did not improve in vitro fertilisation.