ABSTRACT
Abiotic stress is one of the major constraints which restrain plant growth and productivity by disrupting physiological processes and stifling defense mechanisms. Hence, the present work aimed to evaluate the sustainability of bio-priming salt tolerant endophytes for improving plant salt tolerance. Paecilomyces lilacinus KUCC-244 and Trichoderma hamatum Th-16 were obtained and cultured on PDA medium containing different concentrations of NaCl. The highest salt (500 mM) tolerant fungal colonies were selected and purified. Paecilomyces at 61.3 × 10-6 conidia/ml and Trichoderma at about 64.9 × 10-3 conidia/ml of colony forming unit (CFU) were used for priming wheat and mung bean seeds. Twenty- days-old primed and unprimed seedlings of wheat and mung bean were subjected to NaCl treatments at 100 and 200 mM. Results indicate that both endophytes sustain salt resistance in crops, however T. hamatum significantly increased the growth (141 to 209%) and chlorophyll content (81 to 189%), over unprimed control under extreme salinity. Moreover, the reduced levels (22 to 58%) of oxidative stress markers (H2O2 and MDA) corresponded with the increased antioxidant enzymes like superoxide dismutase (SOD) and catalase (CAT) activities (141 and 110%). Photochemical attributes like quantum yield (FV/FM) (14 to 32%) and performance index (PI) (73 to 94%) were also enhanced in bio-primed plants in comparison to control under stress. In addition, the energy loss (DIO/RC) was considerably less (31 to 46%), corresponding with lower damage at PS II level in primed plants. Also, the increase in I and P steps of OJIP curve in T. hamatum and P. lilacinus primed plants showed the availability of more active reaction centers (RC) at PS II under salt stress in comparison to unprimed control plants. Infrared thermographic images also showed that bio-primed plants were resistant to salt stress. Hence, it is concluded that the use of bio-priming with salt tolerant endophytes specifically T. hamatum can be an effective approach to mitigate the salt stress cosnequences and develop a potential salt resistance in crop plants.
ABSTRACT
The sunflower (Helianthus annus L.) is a vital oilseed crop exposed to drought globally. A vast proportion of research is devoted to the naturally occurring microbes and their interaction with plants to alleviate stress consequences. Halophilic bacterial strains, i.e., Bacillus cereus KUB-15 (accession number NR 074540.1), KUB-27 (accession number NR 074540.1), and Bacillus licheniformis strain AAB9 (accession number MW362506), were isolated. Later, isolated strains were used for sunflower through inoculation. Plants were allowed to grow, and thirty-days-old plants were exposed to fixed moisture stress (40-45%). The functionality of photosystem II, light-harvesting ability, and physiological tolerance of cultivars were examined. Bacterial strains B. licheniformis sustained substantial electron flow in between photosystem II (PS II) and photosystem I (PS 1) that not only favored the passable photosynthetic performance but also enhanced antioxidant enzyme activity under stress condition. Compared to other halophilic strains, Bacillus licheniformis did manage reasonable relative water content (RWC), chlorophyll content index (CCI) and biomass production under stress condition. In comparison to both sunflower cultivars, bacterial inoculation was greatly restored growth and photosynthetic performance in Agsun-5264 than S-278 under moisture stress environment. Hence, it is suggested that that bacterial strain and plants cultivar compatibility are essential aspect for sustainable agriculture production.
