ABSTRACT
The purpose of this paper is to quantify by in vitro experiment free radical activity in jaw bone demineralization processes. Sample were take and graphic interpolation of calcium ions undertake. The significance of the results is compared with the paper that are published in literature on the same subject.
Subject(s)
Bone Matrix/drug effects , Calcium/metabolism , Hydrogen Peroxide/pharmacology , Maxilla/drug effects , Minerals/metabolism , Oxidants/pharmacology , Animals , Bone Matrix/metabolism , Cattle , Dose-Response Relationship, Drug , Free Radicals/metabolism , In Vitro Techniques , Maxilla/metabolism , Time FactorsABSTRACT
Two different standard ways of DNA extraction (salting out and phenol-chloroform methods) were assayed in order to recovery nucleic acids from dental tissues. The DNA extracted was tested for purity by means of transverse alternating field electrophoresis (TAFE) using Saccharomyces cerevisiae chromosomes as markers. Both extraction methods give similar qualitative and quantitative results being a DNA yield from hard dental tissues approximately 30% of those extracted from the whole tooth. Our results indicate salting out as a preferable method due to its rapidity and usefulness.
Subject(s)
DNA/isolation & purification , Tooth/chemistry , DNA/analysis , Electrophoresis/instrumentation , Electrophoresis/methods , Humans , Spectrophotometry, UltravioletABSTRACT
After having considered the elements which can rightly address to a precox diagnosis of the multiple myeloma, the AA. analyse the principal laboratory exams. Then, they refer about different therapeutic certificates underlining that the obtained results by the Prof. Reizenstein and Mathè teams are nearly corresponding. They discuss about the compositions and posologies of the most present therapeutic certificates underlining the importance of a precox diagnosis.
