ABSTRACT
BACKGROUND: Mast cells are one of the characteristic factors in angiogenesis, growth, and metastatic spread of tumors. Further studies are suggested to determine the type of these cells which might be useful in the assessment of biological nature of the tumor and its future treatment modality. Few studies have evaluated mast cell infiltration in visceral tumors, especially uterine tumors. CASE PRESENTATION: In this study, age, sex, death rate, and histologic patterns were in agreement with those of previous reports on canine mast cell tumors. Cytopathology assays are widely used to prognosticate canine uterine mast cell tumors (MCT). There is limited information about these prognostic assays used on MCT that arise in the uterine. The anisocytosis and anisocytosis and giant cells were present in the tumor. Furthermore, the tumor had nuclear atypia with scattered multinucleated cells and prominent nucleoli and tumor were classified as poorly granulated. Under microscopic examination, we observed diffuse infiltration and proliferation of tumor cells from the uterine different area and the infiltrative characteristics and distribution patterns of neoplastic cells were observed. This tumor consisted of sheets and cords of uniform round cells with discrete cytoplasmic margins. Microscopically, the neoplastic masses were poorly-demarcated and lacked capsules and tumor cell usually showed a distinct cell boundary. Nevertheless, the neoplastic cells were located between collagen bundles forming small clusters and sheets and had large, centrally located, round to ovoid nuclei. In addition, eosinophils were scattered among the mast cells at the periphery of the masses. The presence of eosinophils and the observation, at high magnification, of cells with cytoplasmic metachromatic granules. CONCLUSION: Based on these findings, a diagnosis of poorly-differentiated mast cell tumor was made and data histologic grading was available for tumor. Neoplasm was poorly differentiated or gradeIII.
Subject(s)
Dog Diseases/diagnosis , Mastocytosis/veterinary , Uterine Neoplasms/veterinary , Animals , Dog Diseases/pathology , Dogs , Female , Mastocytosis/diagnosis , Mastocytosis/pathology , Uterine Neoplasms/diagnosis , Uterine Neoplasms/pathologyABSTRACT
Impaired abomasal motility is common in cattle with abomasal disorders. The macrolide erythromycin has been demonstrated to be an effective prokinetic agent in healthy calves and in adult cattle with abomasal volvulus or left displaced abomasum. We hypothesized that 2 structurally related macrolides, spiramycin and tulathromycin, would also be effective prokinetic agents in cattle. Six milk-fed, male, Holstein-Friesian calves were administered each of the following 4 treatments: spiramycin, 75 000 IU/kg BW, IM, this dose approximates 25 mg/kg BW, IM; tulathromycin, 2.5 mg/kg BW, SC; 2 mL of 0.9% NaCl (negative control); and erythromycin, 8.8 mg/kg BW, IM (positive control). Calves were fed 2 L of cow's milk containing acetaminophen (50 mg/kg body weight) 30 min after each treatment was administered and jugular venous blood samples were obtained periodically after the start of sucking. Abomasal emptying rate was assessed by the time to maximal plasma acetaminophen concentration. Spiramycin, tulathromycin, and the positive control erythromycin increased abomasal emptying rate compared to the negative control. We conclude that the labeled antimicrobial dose of spiramycin and tulathromycin increases the abomasal emptying rate in healthy milk-fed calves. Additional studies investigating whether spiramycin and tulathromycin exert a prokinetic effect in adult cattle with abomasal hypomotility appear indicated.
Un dérèglement de la motilité de l'abomasum est fréquent chez les bovins avec des troubles de l'abomasum. L'érythromycine, un antibiotique de la famille des macrolides, a été reconnu comme un agent procinétique efficace chez les veaux en santé et chez les bovins adultes avec un volvulus de l'abomasum ou un déplacement à gauche de la caillette. Nous avons émis l'hypothèse que deux autres macrolides apparentés structurellement, la spiramycine et la tulathromycine, seraient également des agents procinétiques efficaces chez les bovins. Six veaux mâles de race Holstein-Friesian nourris au lait ont reçu chacun des quatre traitements suivants : spiramycine, 75 000 IU/kg de poids corporel (BW), par voie intramusculaire (IM), cette dose équivaut approximativement à 25 mg/kg BW, IM; tulathromycine, 2,5 mg/kg BW, par voie sous-cutanée (SC); 2 mL d'une solution de NaCl 0,9 % (témoin négatif); et érythromycine 8,8 mg/kg BW, IM (témoin positif). Les veaux ont reçu 2 L de lait de vache contenant de l'acétaminophène (50 mg/kg de poids corporel) 30 min après l'administration de chaque traitement et des échantillons de sang veineux ont été obtenus périodiquement après le début de la tétée. Le rythme de vidange de l'abomasum a été évalué par le temps requis pour atteindre la concentration plasmatique maximale d'acétominophène. La spiramycine, la tulathromycine, et le témoin positif érythromycine ont fait augmenter le rythme de vidange de l'abomasum comparativement au témoin négatif. Nous concluons que la dose antimicrobienne de spiramycine et de tulathromycine mentionnée sur l'étiquette augmente le rythme de vidange de l'abomasum chez des veaux en santé nourris au lait. Des études supplémentaires semblent indiquées pour évaluer si la spiramycine et la tulathromycine ont un effet procinétique chez les bovins adultes avec hypomotilité de l'abomasum.(Traduit par Docteur Serge Messier).
