ABSTRACT
The purpose of this work was to evaluate chemical constituents, antioxidant and antimicrobial activities of Artabotrys hildebrandtii, an endemic medicinal plant from Madagascar. Ethanol extracts from the leaves and stem bark were tested to evaluate DPPH free radical scavenging, using butylated hydroxytoluene and quercetin as standard antioxidants. An high-performance liquid chromatography/mass spectrometry method was developed to investigate the presence of phenolic compounds in the studied samples; gentisic acid, chlorogenic acid, hyperoside, isoquercitrin, rutin, quercitrin, quercetol, apigenin and luteolin were identified. Total polyphenolic content was determined by a spectrophotometric method using Folin-Ciocâlteu reagent. Results showed the efficiency of A. hildebrandtii leaves extract against strains of Staphylococcus aureus and Listeria monocytogenes, as the inhibitory activity is more powerful compared to Gentamicin, used as the standard drug. The leaves of A. hildebrandtii can be considered an important source of polyphenols, especially of rutin, with good antioxidant and antimicrobial activities.
Subject(s)
Annonaceae/chemistry , Anti-Bacterial Agents/chemistry , Antioxidants/chemistry , Plant Extracts/chemistry , Polyphenols/chemistry , Chromatography, High Pressure Liquid , Disk Diffusion Antimicrobial Tests , Listeria monocytogenes/drug effects , Madagascar , Mass Spectrometry , Plant Bark/chemistry , Plant Leaves/chemistry , Plants, Medicinal/chemistry , Staphylococcus aureus/drug effectsABSTRACT
From the stem bark of a Madagascan endemic plant, Millettia richardiana Baill., lonchocarpenin and betulinic acid were isolated and their structures established by spectroscopic methods. The analysis of dichloromethane fractions suggested the presence of beta-amyrin, lupeol, palmitic acid, linoleic acid and stearic acid. Except for beta-amyrin and lupeol, these compounds are described for the first time for the Millettia genus.
