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1.
Macromol Biosci ; 20(3): e1900276, 2020 03.
Article in English | MEDLINE | ID: mdl-31917515

ABSTRACT

It is reported that low concentration of amphiphilic triblock copolymers of pMeOx-b-pTHF-b-pMeOx structure (TBCPs) improves gene expression in skeletal muscle upon intramuscular co-injection with plasmid DNA. Physicochemical studies carried out to understand the involved mechanism show that a phase transition of TBCPs under their unimer state is induced when the temperature is elevated from 25 to 37 °C, the body temperature. Several lines of evidences suggest that TBCP insertion in a lipid bilayer causes enough lipid bilayer destabilization and even pore formation, a phenomenon heightened during the phase transition of TBCPs. Interestingly, this property allows DNA translocation across the lipid bilayer model. Overall, the results indicate that TBCPs exhibiting a phase transition at the body temperature is promising to favor in vivo pDNA translocation in skeletal muscle cells for gene therapy applications.


Subject(s)
DNA , Muscle, Skeletal/metabolism , Plasmids , Transfection , Animals , DNA/genetics , DNA/pharmacology , Female , Mice , Plasmids/genetics , Plasmids/pharmacology
2.
Eur J Pharm Biopharm ; 117: 168-181, 2017 Aug.
Article in English | MEDLINE | ID: mdl-28427956

ABSTRACT

Neutral amphiphilic triblock ABA copolymers are of great interest to solubilize hydrophobic drugs. We reported that a triblock ABA copolymer consisting of methyl-2-oxazoline (MeOx) and tetrahydrofuran (THF) (MeOx6-THF19-MeOx6) (TBCP2) can solubilize curcumin (Cur) a very hydrophobic molecule exhibiting multiple therapeutic effects but whose insolubility and low stability in water is a major drawback for clinical applications. Here, we provide evidences by flow cytometry and confocal microscopy that Cur penetration in normal and ΔF508-CFTR human airway epithelial cell lines is facilitated by TBCP2. When used on ΔF508-CFTR cell lines, the Cur/TBCP2 formulation promotes the restoration of the expression of the CFTR protein in the plasma membrane. Furthermore, patch-clamp and MQAE fluorescence experiments show that this effect is associated with a correction of a Cl- selective current at the membrane surface of F508del-CFTR cells. The results show the great potential of the neutral amphiphilic triblock copolymer MeOx6-THF19-MeOx6 as carrier for curcumin in a Cystic Fibrosis context. We anticipate that other MeOxn-THFm-MeOxn copolymers could have similar behaviours for other highly insoluble therapeutic drugs or cosmetic active ingredients.


Subject(s)
Cell Membrane Permeability/physiology , Curcumin/metabolism , Cystic Fibrosis Transmembrane Conductance Regulator/metabolism , Furans/metabolism , Oxazoles/metabolism , Respiratory Mucosa/metabolism , Animals , Cell Line , Cell Membrane Permeability/drug effects , Curcumin/chemistry , Curcumin/pharmacology , Dose-Response Relationship, Drug , Drug Compounding , Furans/chemistry , Furans/pharmacology , Humans , Mice , Oxazoles/chemistry , Oxazoles/pharmacology , Respiratory Mucosa/drug effects
3.
Biomacromolecules ; 16(3): 748-56, 2015 Mar 09.
Article in English | MEDLINE | ID: mdl-25517924

ABSTRACT

Block copolymers assembled into micelles have gained a lot of attention to improve drug delivery. The recent drawbacks of the poly(ethylene oxide) blocks (PEO) contained in amphiphilic pluronics derivatives made of a central poly(propylene oxide) block surrounded by two PEO blocks were recently revealed, opening the way to the design of new amphiphilic block copolymers able to self-assemble in water and to entrap molecules of interest. Here, a family of p(methyloxazoline)-b-p(tetrahydrofuran)-b-p(methyloxazoline) triblock copolymers (called TBCP) is synthesized using cationic ring opening polymerization. Studies of micelle formation using dynamic light scattering, isothermal titration calorimetry (ITC), NMR diffusion-ordered spectroscopy (DOSY), and fluorescence experiments lead us to draw a relationship between copolymer structure and the physicochemical properties of the block copolymers (critical micellar concentration (CMC), Nagg, core diameter, shell thickness, etc.). The packing parameter of the block copolymers indicates the formation of a core-corona structure. Hydrosolubilizing properties of TBCPs were exemplified with curcumin selected as a highly insoluble drug model. Curcumin, a natural polyphenolic compound, has shown a large spectrum of biological and pharmacological activity, including anti-inflammatory, antimicrobial, antioxidant, and anticarcinogenic activities. An optimized formulation process reveals that the aggregation number is the parameter affecting drug encapsulation. Patch clamp experiments carried out to study the interaction of TBCP with the cell membrane demonstrate their permeation property suitable to promote the cellular internalization of curcumin.


Subject(s)
Butylene Glycols/chemical synthesis , Polyamines/chemical synthesis , Polymers/chemical synthesis , Surface-Active Agents/chemical synthesis , Curcumin/chemistry , Curcumin/metabolism , Drug Carriers/chemistry , Drug Carriers/metabolism , HEK293 Cells , HeLa Cells , Humans , Hydrophobic and Hydrophilic Interactions , Micelles , Solubility
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