ABSTRACT
About 4% to 7% of the non-small-cell lung cancer patients have anaplastic lymphoma kinase (ALK) rearrangements, and specific targeted therapies improve patients' outcomes significantly. ALK gene fusions are detected by immunohistochemistry or fluorescent in situ hybridization as gold standards in South America. Next-generation sequencing-based assays are a reliable alternative, able to perform simultaneous detection of multiple events from a single sample. We analyzed 4240 non-small-cell lung cancer samples collected in 37 hospitals from Chile, Brazil, and Peru, where ALK rearrangements were determined as part of their standard of care (SofC) using either immunohistochemistry or fluorescent in situ hybridization. A subset of 1450 samples was sequenced with the Oncomine Focus Assay (OFA), and the concordance with the SofC tests was measured. An orthogonal analysis was performed using a real-time quantitative PCR echinoderm microtubule-associated protein-like 4-ALK fusion detection kit. ALK fusion prevalence is similar for Chile (3.67%; N = 2142), Brazil (4.05%; N = 1013), and Peru (4.59%; N = 675). Although a comparison between OFA and SofC assays showed similar sensitivity, OFA had significantly higher specificity and higher positive predictive value, which opens new opportunities for a more specific determination of ALK gene rearrangements.
Subject(s)
Anaplastic Lymphoma Kinase/genetics , Carcinoma, Non-Small-Cell Lung/genetics , Gene Fusion , High-Throughput Nucleotide Sequencing/methods , Lung Neoplasms/genetics , Oncogene Proteins, Fusion/genetics , Adult , Aged , Aged, 80 and over , Brazil/epidemiology , Carcinoma, Non-Small-Cell Lung/epidemiology , Chile/epidemiology , Female , Gene Rearrangement , Humans , Immunohistochemistry/methods , In Situ Hybridization, Fluorescence/methods , Lung Neoplasms/epidemiology , Male , Middle Aged , Peru/epidemiology , Prospective Studies , Retrospective Studies , Standard of Care , Young AdultABSTRACT
We report a previously healthy 36-year-old mole who developed a pulmonary syndrome caused by Hantavirus infection. He was admitted to an intensive care unit in shock and respiratory failure and died four hours after admission. Blood cultures were positive for Staphylococcus aureus. This patient could be an index case that shows, for the first time, an association of Hantavirus Pulmonary Syndrome with Staphylococcus aureus infection similar to what happens with other viruses.
Subject(s)
Hantavirus Pulmonary Syndrome/complications , Sepsis/microbiology , Staphylococcal Infections/complications , Staphylococcus aureus , Adult , Fatal Outcome , Hantavirus Pulmonary Syndrome/pathology , Humans , Male , Sepsis/pathology , Staphylococcal Infections/pathologyABSTRACT
We report a previously healthy 36-year-old mole who developed a pulmonary syndrome caused by Hantavirus infection. He was admitted to an intensive care unit in shock and respiratory failure and died four hours after admission. Blood cultures were positive for Staphylococcus aureus. This patient could be an index case that shows, for the first time, an association of Hantavirus Pulmonary Syndrome with Staphylococcus aureus infection similar to what happens with other viruses.
