[Cross reactivity and amino acid structural homology of immunodominant peptide fragments of structural viruses from the human retroviruses HTLV-I, HTLV-II, HIV-1, and HIV-2]. / Perekrestnaia reaktivnost' i gomologiia aminokislotnoi struktury immunodominantnykh peptidnykh fragmentov strukturnykh belkov retrovirusov cheloveka HTLV-1, HTLV-II, HIV-1 i HIV-2.

Twelve synthetic peptides corresponding to 9 immunodominant regions of structural proteins of human retroviruses HTLV-I, HIV-1, and HIV-2 were studied in enzyme-linked immunosorbent assay for cross reactivity with heterotypical for each peptide anti-retroviral antibodies. The search of amino acid homologies was carried using the special computer program followed by the correspondence analysis of the discovered homologies and immunodominant fragments. It was found that peptides 100-130 p19 gag HTLV-I, 376-392 gp21 env HTLV-I, 381-400 gp21 env HTLV-I, 306-328 gp120 env HIV-1, 495-516 gp120 env HIV-1, 584-612 gp41 env HIV-1, and 581-603 gp36 env HIV-2 have type-specific reactivity and also cross react with 3-54% human sera containing antibodies against heterotypical retroviruses. On the other hand, peptides 120-130 p19 gag HTLV-I, 176-201 gp46 env HTLV-I, 291-312 gp46 env HTLV-I, 330-363 p24 gag HIV-1, and 602-624 gp41 env HIV-1 have shown no cross reactive properties; they may be effectively used for type-specific and differential serodiagnosis of human retroviral infections.

[A synthetic peptide-based immunoenzyme test system for the joint detection of human retrovirus (HTLV-1 and HIV-1) antibodies]. / Immunofermentnaia test-sistema dlia sochetannogo vyiavleniia antitel k retrovirusam cheloveka (HTLV-1 i HIV-1) na osnove sinteticheskikh peptidov.

The study was made to develop an immunodiagnostic test system based on synthetic peptides able to detect in the same assay the total concentration of heterospecific antibodies against human retroviruses HTLV-1 and HIV-1. Three panels of reference-sera contained antibodies to HTLV-1 (70 specimens), HIV-1 (50 and 16 specimens) and 4 synthetic peptides corresponding to protein fragments p19 gag and gp46 env HTLV-1, gp120 and gp41 env HIV-1. Immune reactivity of the peptides with reference sera was measured in the immunoassay. It is established that relevant peptides mimic immunodominant B-epitopes of structural proteins HTLV-1 and HIV-1 and are recognized specifically by relevant antiviral antibodies. The enzyme immunoassay test system has been designed using a peptide combination in a single antigen complex. The system showed high diagnostic sensitivity.