Temperature-induced reversible changes in photosynthesis efficiency and organization of thylakoid membranes from pea (Pisum sativum).

High temperature can induce a substantial adverse effect on plant photosynthesis. This study addressed the impact of moderately high temperature (35 °C) on photosynthetic efficiency and thylakoid membrane organization in Pisum sativum. The Chl a fluorescence curves showed a significant change, indicating a reduction in photosynthetic efficiency when pea plants were exposed to moderate high-temperature stress. The pulse-amplitude modulation measurements showed decreased non-photochemical quenching while the non-regulated energy dissipation increased in treated compared to control and recovery plants. Both parameters indicated that the photosystem (PS)II was prone to temperature stress. The PSI donor side limitation increased in treated and recovery plants compared to control, suggesting the donor side of PSI is hampered in moderate-high temperature. Further, the PSI acceptor side increased in recovery plants compared to control, suggesting that the cyclic electron transport is repressed after temperature treatment but revert back to normal in recovery conditions. Also, the content of photoprotective carotenoid pigments like lutein and xanthophylls increased in temperature-treated leaves. These results indicate the alteration of macro-organization of thylakoid membranes under moderately elevated temperature, whereas supercomplexes restored to the control levels under recovery conditions. Further, the light harvesting complex (LHC)II trimers, and monomers were significantly decreased in temperature-treated plants. Furthermore, the amount of PSII reaction center proteins D1, D2, PsbO, and Cyt b6 was reduced under moderate temperature, whereas the content of LHC proteins of PSI was stable. These observations suggest that moderately high temperature can alter supercomplexes, which leads to change in the pigment-protein organization.

Change in the photochemical and structural organization of thylakoids from pea (Pisum sativum) under salt stress.

Salt can induce adverse effects, primarily on the photosynthetic process, ultimately influencing plant productivity. Still, the impact of salt on the photosynthesis process in terms of supercomplexes organization of thylakoid structure and function is not understood in Pea (Pisum sativum). To understand the structure and function in the leaves and thylakoids under salt (NaCl) treatment, we used various biophysical and biochemical techniques like infrared gas analyzer, chlorophyll a fluorescence, circular dichroism, electron microscopy, blue native gels, and western blots. The net photosynthetic rate, transpiration rate, and stomatal conductance were reduced significantly, whereas the water use efficiency was enhanced remarkably under high salt conditions (200 mM NaCl). The photochemical efficiency of both photosystem (PS) I and II was reduced in high salt by inhibiting their donor and acceptor sides. Interestingly the non-photochemical quenching (NPQ) is reduced in high salt; however, the non-regulated energy dissipation (NO) of PSII increased, leading to inactivation of PSII. The obtained results exhibit inhibition of NAD(P)H dehydrogenase (NDH) mediated pathway-dependent cyclic electron transport under salinity caused a decrease in proton motive force of ΔpH and Δψ. Further, the electron micrographs show the disorganization of grana thylakoids under salt stress. Furthermore, the macro-organization and supercomplexes of thylakoids were significantly affected by high salt. Specifically, the mega complexes, PSII-LHCII, PSI-LHCI, and NDH complexes were notably reduced, ultimately altering the electron transport. The reaction center proteins of oxygen-evolving complexes, D1 and D2 proteins were affected to high salt indicating changes in photochemical activities.