Global view of vasomotor symptoms and sleep disturbance in menopause: a systematic review.

Studies have shown racial/ethnic differences in the prevalence of vasomotor symptoms (VMS), sleep disturbance and VMS treatment in menopause. To assess the reproducibility of these differences, we systematically reviewed observational studies, published in 2000-2021, reporting the prevalence/incidence of VMS, sleep disturbance or treatment use in menopausal women stratified by race/ethnicity. We screened 3799 records from PubMed and Embase and included 27 papers (19 studies). No incidence data were found. Prevalence data varied widely, but some common patterns emerged. In all five studies comparing VMS between Black women and White, Hispanic and/or East Asian women, the prevalence was highest in Black women and lowest in East Asian women. The prevalence of sleep disturbance overall was compared among Black, White and East Asian women in two study populations, and was highest in White women in both papers. Sleep disturbance was more common than VMS in East Asian women. In all four studies comparing hormone therapy use between White women and Black and/or East Asian women, treatment use was more common in White women. These results highlight the need for individualized counseling and treatment, outreach to under-served minorities, and standardized definitions and outcome measures for VMS and sleep disturbance for future studies.

Denervation of the thumb carpometacarpal joint for symptomatic osteoarthritis in 46 patients.

We investigated the effect of an extended denervation procedure in the thumb carpometacarpal (CMC) joint in patients suffering from CMC osteoarthritis. Between 2006 and 2018, 46 patients underwent the procedure in our clinic and were included in this retrospective study. Pain, strength, range of motion, DASH score, complications and overall satisfaction were determined. Assessment showed a significant decrease in pain and excellent physical function at a median 5 years' follow-up. Twelve patients needed secondary surgery due to persistent pain. Overall, 28 of the 46 patients were satisfied with the results of the denervation. Even though the results of CMC denervation are poorer than with simple trapeziectomy, considerable pain relief can be achieved in selected young, physically active patients by exclusively soft-tissue surgery, conserving the biomechanical properties of the joint. In case of failure of the procedure, all other options remain available.

Epidemiology of uterine fibroids: a systematic review.

BACKGROUND: Uterine fibroids (UFs) are the most common neoplasm affecting women that can cause significant morbidity and may adversely impact fertility. OBJECTIVES: To examine UF epidemiology and to evaluate the relative strengths of putative risk factors. SEARCH STRATEGY: MEDLINE and Embase were searched for studies published in English between January 1995 and April 2015. SELECTION CRITERIA: Publications reporting relevant data from registries and other observational studies with over 1000 patients and single-centre studies with over 100 patients were selected. DATA COLLECTION AND ANALYSIS: Data on UF incidence, prevalence and associated risk factors were extracted from 60 publications. MAIN RESULTS: Wide ranges were reported in both UF incidence (217-3745 cases per 100 000 women-years) and prevalence (4.5-68.6%), depending on study populations and diagnostic methods. Black race was the only factor that was recurrently reported to increase UF risk, by two-threefold compared with white race. Eleven other factors affected UF risk to a magnitude similar to or greater than race. Age, premenopausal state, hypertension, family history, time since last birth, and food additive and soybean milk consumption increased UF risk; use of oral contraceptives or the injectable contraceptive depot medroxyprogesterone acetate, smoking in women with low body mass index and parity reduced UF risk. CONCLUSIONS: We identified 12 risk factors that play an important role in UF epidemiology. The UF risk factor with the strongest evidence is black race. High-quality prospective observational data are needed to improve our understanding of UF epidemiology, and thus its aetiology and optimal management. TWEETABLE ABSTRACT: Uterine fibroids occur in about 70% of women. Black race and 11 other factors affect uterine fibroid risk.

Long-Term Respiratory Support for Children and Adolescents in Austria: A National Survey.

BACKGROUND: Population-based data on pediatric patients on long-term respiratory support (LTRS) in Austria are lacking. This study aimed to record the pediatric departments active in this field, as well as number and characteristics of patients on LTRS. METHODS: A national cross-sectional study was carried out by means of questionnaires sent to all pediatric departments in Austria. RESULTS: All departments answered to the questionnaires. On June 1st, 2013, the reference day for this study, 12 of the 41 pediatric departments in Austria were active in the field. At this time, these centers were caring for 143 patients, 111 (77.6%) of them under 18 years, which corresponds to a prevalence of 7.4 per 100 000. The patients suffered from neuromuscular disorders (44%), other neurological disorders (18.9%), disorders of respiratory drive (9.1%), obstructive sleep apnea (8.4%), thoracal and spinal diseases (8.4%), pulmonary disorders (4.9%) and other diseases (6.3%). Continuous positive airway pressure was used in 6.3%, non-invasive ventilation in 60.1% and invasive ventilation in 33.6% of the patients, respectively. LTRS was performed at home in 92.3%. CONCLUSION: LTRS represents a common management strategy in children and adolescents with a variety of disorders. Census reports such as this one provide the basis for appropriate planning of resource allocation. The age distribution of our patients shows the need for structured transition into adult care.

[Metaphyseal fitting short-stem prosthesis - preliminary results of a prospective single-surgeon series]. / Metaphysär verankernde, zementfreie Kurzschaftprothese - klinisch-radiologische Ergebnisse einer prospektiven Single-Surgeon-Studie.

BACKGROUND: Conventional uncemented femoral stems provide good long-term fixation in patients with a wide range of clinical function. However, preservation of bone stock, and minimally invasive approaches have led to exploration into various other implant designs. Short-stem prosthesis focusing on a stable metaphyseal fit have emerged to address these challenges in total hip arthroplasty. The purpose of this study was to evaluate the clinical and radiological results of a newly developed short-stem hip prosthesis AIDA® in context of a single surgeon study. MATERIAL AND METHODS: From February 2009 to December 2013, 72 cementless "AIDA® short stems" (Fa. Implantcast) were implanted by a single surgeon in one study centre via the Watson Jones interval. All patients signed informed consent prior to screening and the study design was approved by the local ethics committee. 51 patients with 52 implants (35 female, 36 male) with a follow-up > 24 months were included in this prospective, controlled clinical trial. The patients were pre- and postoperatively examined clinically and radiological by a specified protocol. RESULTS: The average patient age at the time of THA was 61 ± 3.2 years (min.-max. 46-68 years). At the last follow-up, the average Harris Hip score increased from 41.4 ± 4.5 preoperatively to 96.8 ± 3.2 points postoperatively. The hospital stay was 9.1 days on average. The X-rays showed in all cases a stable fixation of the stems with full bony integration and no signs of loosening or migration. There were no specific complications relating to the less invasive approach. Postoperatively one periprosthetic fracture was evident. The revision operation into a cementless stem was done without any complications (revisions rate 1.9 %). CONCLUSION: The newly developed "AIDA short stem" is a promising hip implant for the young and active patient with osteoarthritis of the hip. The short-term results are encouraging, but nevertheless mid- and long-term results must be further observed on a prospective basis as part of this collective study.

Alteration of biomechanical properties of skin in acute cold contact injury.

BACKGROUND: The prevalence of acute cold injury has increased recently. Despite new research findings, these injuries and their resulting tissue damage are still not entirely understood. Especially, little is known about alteration of skin biomechanical properties. METHODS: A total of 36 acute cold contact wounds with different depths were generated on the abdomen of six Göttingen minipigs. Alteration of biomechanical properties of skin was evaluated objectively after 15 and 360 min using a Cutometer device. Biopsies for histological evaluation were taken, and the depth of injury was correlated with biomechanical properties. RESULTS: Calculated elasticity (Ue), firmness of skin (R0) and overall elasticity (R8) demonstrated a continuous decrease, whereas other parameters demonstrated an initial increase with increasing depth of injury 15 min after wound generation. All parameters showed an increase compared to healthy skin, 360 min after wound generation. Furthermore, an alteration of values over time was detected. CONCLUSION: Alteration of biomechanical properties of skin is a function of damaged tissue structures. The presented results demonstrate a decrease of main elastic parameters with increasing depth of injury and indicate progressive tissue damage over time. Skin elasticity measurements are a valuable tool in acute cold contact injury depth assessment and may act as an influencing factor in management decisions.

Platelet adhesion and human umbilical vein endothelial cell cytocompatibility of biodegradable segmented polyurethanes prepared with 4,4'-methylene bis(cyclohexyl isocyanate), poly(caprolactone) diol and butanediol or dithioerythritol as chain extenders.

Biodegradable segmented polyurethanes were prepared with poly(caprolactone) diol as a soft segment, 4,4'-methylene bis(cyclohexyl isocyanate) (HMDI) and either butanediol or dithioerythritol as chain extenders. Platelet adhesion was similar in all segmented polyurethanes studied and not different from Tecoflex® although an early stage of activation was observed on biodegradable segmented polyurethane prepared with dithioerythritol. Relative viability was higher than 80% on human umbilical vein endothelial cells in contact with biodegradable segmented polyurethane extracts after 1, 2 and 7 days. Furthermore, both biodegradable segmented polyurethane materials supported human umbilical vein endothelial cell adhesion, spreading, and viability similar to Tecoflex® medical-grade polyurethane. These biodegradable segmented polyurethanes represent promising materials for cardiovascular applications.

Cell-free repair of small cartilage defects in the Goettinger minipig: which defect size is possible?

PURPOSE: Cartilage repair of full-thickness chondral defects in the knees of Goettinger minipigs was assessed by treatment with cell-free collagen type-I gel plugs of three different sizes. METHODS: In 6 adult Goettinger minipigs, three full-thickness chondral defects were created in the trochlear groove of one knee of the hind leg. These defects were treated with a cell-free collagen type-I gel plug of 8, 10, or 12 mm diameter. All animals were allowed unlimited weight bearing. After 1 year, the animals were killed. Immediately after recovery, a non-destructive biomechanical testing was performed. The repair tissue quality was evaluated immunohistologically, collagen type-II protein was quantified, and a semiquantitative score (O'Driscoll score) was calculated. RESULTS: After 1 year, a high number of cells migrated into the initially cell-free collagen gel plugs and a hyaline-like repair tissue had been created. The O'Driscoll scores were: 8 mm, 21.2 (SD, 2.8); 10 mm, 21.5 (SD, 1.6); and 12 mm, 22.3 (SD, 1.0). The determination of the e-modulus, creep and relaxation revealed that mechanical properties of the two smaller defects were closer to unaffected hyaline cartilage. CONCLUSIONS: As cell-free collagen type-I gel plugs of all three different sizes created hyaline-like repair tissue, this system seems suitable for the treatment of even larger defects.

[Tissue engineering of cartilage replacement material - mechanical stimulation in the in-vitro cultivation of human chondrocytes]. / Tissue Engineering von Knorpelersatzgewebe - Mechanische Stimulation in der In-vitro-Kultivierung von humanen Chondrozyten.

The treatment of cartilage defects remains a major problem in orthopaedics. With regard to cartilage tissue engineering, the reimplantation of pre-cultivated chondrocytes in the form of a chondrocyte graft is a promising alternative to conventional methods. Clinical practice requires this MACT procedure (matrix-associated autologous chondrocyte transplantation) to produce a biocompatible replacement tissue with adequate mechanical properties. Mechanical stimulation has the capacity to improve the quality of these cell-seeded biomaterials. By altering chondrocytes' cellular activities, the biological and biomechanical properties of cartilage replacement tissue can be modulated. Different systems are used for this purpose, e.g. shear, perfusion, hydrostatic pressure or compression. The mechanisms, biological effects, chances and problems of the techniques are presented and assessed. Among the stimulating techniques considered are systems that apply indirect and direct shear forces such as spinner flasks, rotating-wall bioreactors, direct tissue shear and perfusion culture systems. The application of hydrostatic pressure or compression may be brought about by either static or dynamic loading systems. Compressive loading is considered in the light of both its short- and long-term effects; additionally two exemplified systems are discussed in detail. However, despite promising approaches and seemingly favourable tissue characteristics, the in vitro culturing of functional cartilage replacement tissue with cartilage-like mechanical and biological characteristics still remains elusive. Furthermore, controlling, monitoring and regulating culturing conditions are general biotechnological requirements of a standardised in vitro cultivation. Among these, different aspects such as aseptic operation, media supplementation, nutrient and gas exchange, temperature and humidity control are considered.

[Clinical and gait analytical investigation of valgus knee bracing in therapy for medial degenerative joint disease of the knee]. / Klinische und ganganalytische Untersuchung einer valgisierenden Kniegelenkentlastungsorthese in der Therapie der medialen Gonarthrose.

GOALS: The non-operative treatment of medial degenerative joint disease of the knee has proven to be difficult due to the underlying deformity and the pathological mechanical loading. Valgus knee bracing offers the possibility to directly address the mechanical deficit. The aim of this study was to analyse whether or not part-time valgus knee bracing in patients with medial osteoarthrits can effectively reduce knee pain and lead to reproducible changes in the gait analysis in comparison to an elastic knee bandage and an untreated control group. MATERIAL AND METHODS: In a prospective trial, 33 patients with symptomatic medial osteoarthritis of the knee joint with a minimum of grade 2 according to the radiographic classification of Kellegren & Lawrence were randomised into a treatment group with a valgus brace (n = 13; M4 OA®, Medi) and an elastic knee bandage (n = 10; Genumedi®, Medi). Both supportive devices were to be applied for 2-4 hours per day, especially during activity. The control group (n = 10) consisted of untreated individuals. The deviation of the leg axis and the degree of degenerative joint disease were evaluated radiographically at the onset of the study by a standing whole leg X-ray. The evaluation at the beginning of the study and after 16 weeks consisted of a clinical examination including various knee scores (Insall score, Lequesne score, HSS score, Tegner score, WOMAC, and VAS for pain). Additionally, at both times a 3-dimensional, instrumented gait analysis was carried out to document the joint angles of the affected knee in all planes. The obtained knee joint angles from all groups at both time points were compared to a control group of healthy persons without a history of knee pain by qualitative measurement. RESULTS: Radiographically, the medial deviation of the load axis from the knee joint centre (MAD) was 29 mm. In 27 patients we found a combination of femoral, intra-articular and tibial changes contributing to the varus alignment. The joint line conversion angle (JLCA) was pathological in all subjects. Clinically, in 4 of 5 subjective and objective scores (Insall score, Lequesne score, Tegner score, WOMAC, and VAS for pain) a statistically significant improvement of the symptoms, joint function and activity level thanks to the application of the corrective valgus knee brace was documented after 16 weeks. The knee bandage also led to an improvement, but only in 2 scores (Insall score, Tegner score). No changes were documented for the control group. The gait analysis of the orthosis group revealed an improvement in the extension and flexion angles (sagittal plane) and the knee rotation angles (horizontal plane) when compared to a control group. CONCLUSION: The correction obtained by this knee orthosis, which places a valgus stress on the medially osteoarthritic knee, is an effective addition to the conservative treatment protocols and is superior to the isolated use of an elastic knee bandage. Further research is warranted to evaluate the longevity of such treatment and to further improve the technique of gait analysis by the development of quantitative parameters.

[Muscle strength of the cervical and lumbar spine in triathletes]. / Das muskuläre Profil der Halswirbelsäule und der Lendenwirbelsäule bei Langstreckentriathleten.

AIM: The goal of this study was to analyse the muscle strength of the cervical and lumbar spine in ironman triathletes. The values were compared to the results obtained from a reference group. The test of the triathletes was carried out in an attempt to define a specific strength profile for these athletes. METHOD: In this study, 20 long-distance triathletes (∅ 37.3 ± 7.6 years of age, ∅ 1.80 ± 0.1 m, ∅ 73.7 ± 6.0 kg) were evaluated with regard to their individual and sport-specific strengths of the cervical spine in 2 planes and of the trunk strengths in all 3 planes of motion. The trunk strength profile of the triathletes revealed good average results in the trunk extensors and the lateral flexors of the left trunk. The reference group is the data base of the company Proxomed®, Alzenau. It is based on results of 1045 untrained, symptom-free subjects of different ages. RESULTS: Lumbar extension: The extension of the force values shows no significant difference from the reference group. Lumbar flexion: The flexion tests show highly significantly lower force values (5.025 ± 0.81 N/kg vs. 6.67 ± 0.6 N/kg) than the reference group. Flexion/extension: In the sagittal plane values for the triathletes demonstrate an imbalance in muscle strength ratios. The abdominal muscles turn in relation to the back extensor muscles too weakly to be very significant. Lumbar rotation: The force values of the athletes in both directions (right: 6.185 ± 1.46 N/kg, left: 7.1 ± 1.57 N/kg vs. 10.05 ± 0.34 N/kg) are highly significantly (p ≤ 0.001) lower than the reference values. Ratio of rotation left/right: The ratio of left/right rotation in the reference group is set at 1 and thus shows an equally strong force level between the two sides. Lumbar lateral flexion: The triathletes do not show any significant differences between the force values. Compared to the reference group there is no significant difference to the left side flexion. In the lateral bending the athletes have significantly better values than the reference group. Ratio of lateral left/right: In the reference group the ratio is set at 1. For triathletes, it shows an average value of 0.93. This difference is not significant. Cervical extension: The extension of the force values (1.96 ± 0.59 N/kg vs. 3.03 ± 0.24 N/kg) shows a highly significant difference from the reference group. Cervical flexion: In flexion (1.3 ± 0.42 N/kg vs. 2.17 ± 0.22 N/kg) triathletes have highly significantly lower strength values than the reference group. Flexion/extension: The triathletes did not differ significantly from the reference values (0.69 ± 0.23 and 0.72 ± 0.08). Lateral cervical spine: In comparison to the reference group (left: 1.67 ± 0.48 N/kg, right: 1.55 ± 0.46 N/kg vs. 2.36 ± 0.15 N/kg) in which there is left/right lateral flexion, there is a highly significant difference. Right lateral flexion is weaker than the left. Ratio of lateral left/right: The triathletes have a significant imbalance in the lateral flexion of the cervical spine compared to the reference group (1.07 ± 0.15 to 1). CONCLUSION: In conclusion, in the triathlon there is a specific stress that is obviously not an adequate stimulus for the muscles of the cervical spine in order to achieve a balanced musculature and the athletes should be advised to practice a preventive approach with regard to these areas.

[Comparison of trunk muscle strength of soccer players with and without low back pain]. / Vergleich der Rumpfmuskelkraft von Fussballspielern mit und ohne Rückenschmerzen.

AIM OF THE STUDY: Low back pain in soccer players is one of the frequently appearing disorders caused by overuse. Myogenic dysbalances are under discussion as possible reason for this problem. In the present study the muscular strength profile of the trunk musculature of soccer players with and without low back pain was evaluated. The results of the asymptomatic players were compared to those of players with low back pain; furthermore, the collected data were compared to those of an asymptomatic reference group. The question posed was whether soccer players show a specific strength profile caused by the special, sports-specific requirements and whether this strength profile differs between players with and without low back pain. METHOD: In the present study the isometric maximal strength of 18 soccer players with and 18 soccer players without low back pain was measured in all 3 planes. The reference group was provided by the Proxomed company, which had previously analysed 1045 healthy untrained individuals of various age groups. RESULTS: The soccer players showed a sport-specific profile for the musculature, which was determined by a significant reduction of the flexion and rotation strength (flex: Ø 5.21 N/kg vs. Ø 6.49 N/kg; Ø 5.78 N/kg vs. Ø 6.66 N/kg respectively; rotation: left 7.09 N/kg, right 8.69 N/kg vs. left/right 10.1 N/kg; left 7.22 N/kg, right 8.24 N/kg vs. left/right 10.0 N/kg, respectively) as well as by an increased lateral flexion strength to the right-hand side in comparison to the reference group (lat. flex. right: 9.87 N/kg, respectively, 10.67 N/kg vs. 8.3 N/kg). A statistically significant correlation between the muscular activity in the trunk stability of soccer players with and without low back pain could not be shown. CONCLUSION: Obviously sports-specific training with additional specific training of the trunk muscles is not sufficient for the development of a balanced strength of trunk musculature. In the present study an influence of the performance of the trunk musculature on the incidence of low back pain could not be shown.

[Perioperative management in outpatient arthroscopy of the knee joint]. / Perioperatives Management in der ambulanten arthroskopischen Chirurgie des Kniegelenks.

AIM: Nowadays, complex arthroscopic procedures of the knee joint, such as ACL reconstruction, are routinely carried out on an outpatient basis. The reduced time spent with the patient places high demands on the surgeon with regard to the management of such cases. The aim of this study was to evaluate the current standards of perioperative management following outpatient arthroscopic surgery of the knee in Germany. METHODS: A questionnaire consisting of 18 questions was sent to 215 members of the German Association of Outpatient Arthroscopy (BVASK e. V.), dealing with the following topics: antibiotic prophylaxis, DVT prophylaxis, use of a tourniquet and suction drain, physical therapy, physiotherapy, analgesia, anaesthesia and emergency management. More than 51% of the forms were returned and considered suitable for statistical analysis. RESULTS: A total of 62% of the surgeons reported the use of an antibiotic prophylaxis (i. v. single shot) in every arthroscopic case, while 19% administer antibiotics only occasionally, especially in ACL reconstruction. Postoperative antibiotic treatment was reserved for special situations, such as prior joint infection. Prophylaxis of DVT by means of low molecular weight heparin was carried out in 96% of the cases. 51% prescribed anticoagulant agents for 1 week, 39% for the duration of reduced weight bearing. Half of the surgeons used a tourniquet cuff, 22% exsanguinate the limb prior to cuff inflation. A suction drainage was applied by 36% of the surgeons regularly and by 45% occasionally. The drain is left for one day by 79% of the surgeons, while 11% reported a shortened use of only several hours. With regard to analgesia, 66% of all surgeons apply some kind of local anaesthetic into the knee joint post surgery. Systemic preoperative analgesics or anti-inflammatory agents are given regularly in 56 % of the cases. A total of 92% of the procedures are carried out under general anaesthesia. In 36% of the cases, some kind of additional regional peripheral anaesthesia is used. With regard to postoperative care, cryotherapy is considered standard (97%) and 64% of the surgeons recommend physiotherapy. Nearly all surgeons (97%) offer the patients the opportunity to reach them personally via mobile phone during the first night following arthroscopic surgery. CONCLUSIONS: DVT prophylaxis with LMWH, general anaesthesia, postoperative cryotherapy and personal availability by phone can be considered part of the standard perioperative management following outpatient knee arthroscopy in Germany. However, a wide variety of treatment options can be found regarding topics such as analgesia, antibiotic prophylaxis, tourniquet, knee drainage and physiotherapy.

Case-control study on risk factors for leukaemia and brain tumours in children under 5 years in Germany.

In the context of a case control study on the cancer risk for children under five by distance to the nearest nuclear power plant, we collected information on other risk factors in a subset. We present the interview study as if it had been an independent study. Parents of 471 cases with Leukaemia, Lymphoma or CNS (Central Nervous System)-tumour from the German Childhood Cancer Registry, diagnosed at age under 5 in the years 1993-2003, and 1,457 matched controls were to be interviewed. For Leukaemia, 243 cases/604 controls, and for CNS 102 cases/246 controls participated, lymphoma cases were too few. Questions related to social status, ionizing radiation, pregnancy and birth, immune system, and selected toxins. The analysis is exploratory in nature; variables were selected by backward elimination. For leukaemia we found a significant protective effect of social contacts (OR=0.50, 95% CI [0.29;0.87]) and a risk for high birth weight (OR=1.96 95% CI [1.12;3.41] comparing >4,000 g to "normal"). We could not reproduce other associations reported in the literature such as a negative association with allergies. For CNS tumours we found a significant protective effect of social contacts (OR=0.30 95% CI [0.13;0.72]), of pesticides and herbicides (OR=0.39 95% CI [0.18;0.83]) and an increased risk for low birth weight (p=0.0232). This study on risk factors for childhood leukaemia and brain tumours is relatively small and exploratory. We could reproduce some major associations reported in the literature (leukaemia: social contacts and high birth weight) but not others. Some observations may be reporting artefacts or self selection artefacts.

[Influence of sex and side dominance on the results of quantitative, three-dimensional motion analysis of the upper extremities]. / Einfluss des Geschlechts und der Seitendominanz auf die Ergebnisse der quantitativen, dreidimensionalen Bewegungsanalyse der oberen Extremitäten.

AIM: Clinical assessment does not provide objective and quantitative evaluation of the shoulder function. Based on instrumental gait analysis a system for three-dimensional motion analysis of the upper extremities has been developed. The aim of this study is to evaluate the results of instrumental upper extremity motion analysis with respect to dependence on sex and side dominance. METHOD: 16 healthy subjects (8 male, 8 female, mean age 26 years) were included. Three-dimensional motion analysis was carried out with a video-based system and passive markers. All subjects performed two movement tasks representing activities of daily living (task A: reaching the neck, task B: removing a parking token). All joint angles of the upper extremity were recorded and graphically displayed. Additionally, a calculation of quantitative parameters was performed. RESULTS: Contrary to expectations the task "reaching the neck" was not combined with external shoulder rotation. Females performed most of the tasks quicker and with higher reproduction accuracy. Movement reproduction accuracy was rarely dependent on dominance. CONCLUSION: Three-dimensional upper extremity motion analysis of activities of daily living allows a precise and quantitative evaluation of movement patterns. The differences with respect to sex and side in a healthy group have to be respected in studies dealing with comparative shoulder function.

[Optical coherence tomography (OCT) to evaluate cartilage tissue engineering]. / Die optische Kohärenztomografie (OCT) zur Darstellung der Regenerateigenschaften im Tissue Engineering von Knorpelgewebe.

AIM: The aim of this study was to investigate hyaline cartilage defects treated with cell-seeded artificial matrix systems (two different collagen type I gels) with the method of optical coherence tomography (OCT) and to correlate the results with conventional histological and immunocytochemical staining. METHOD: Osteochondral blocks were harvested from 20 patients undergoing total knee replacement and trimmed to 2 x 2 cm. Under sterile conditions, chondral defects of 8 mm diameter were either filled with a collagen type I gel plug seeded with autologous chondrocytes (2 x 10 (5)/mL gel), or with a corresponding gel plug which was stabilised by a 20-fold compression. Of each group, 5 specimens were cultivated for 6 weeks under standardised in vitro conditions (37 degrees C, 5 % CO (2), humidified atmosphere), while the remaining 5 specimens were implanted subcutaneously in nude mice (BALBc -/-). Immediately after recovery, the repair tissue and bonding zones were investigated by OCT. Subsequently, specimens were decalcified and investigated by H&E staining and collagen type II immunostaining. The results of OCT and conventional staining were correlated. RESULTS: By OCT, repair tissue could be investigated up to 1.6 mm in depth, physically limited by the utilised OCT system. In the denser hyaline cartilage regions, OCT resolution was reduced. Regardless of cultivation (in vitro or nude mouse), ultrastructural features of the repair tissue could be demonstrated. In particular, ultrastructural differences between the two investigated collagen gels could be detected. Moreover, the bonding region between repair tissue and hyaline cartilage could be evaluated by OCT investigation. The results of the OCT measurements were confirmed by H&E and collagen type II immunostaining. CONCLUSION: By OCT, repair tissue generated by the treatment of cartilage defects with tissue-engineered matrix systems could be evaluated in vitro. Future studies may show if repair tissue quality may be monitored in situ by OCT.

In vitro interface and cement mantle analysis of different femur stem designs.

The aim of this study is to define stem design related factors causing both gaps in the metal-bone cement interface and cracks within the cement mantle. Six different stem designs (Exeter; Lubinus SP II; Ceraver Osteal; Mueller-straight stem; Centega; Spectron EF) (n=15 of each design) were cemented into artificial femur bones. Ten stems of each design were loaded, while five stems served as an unloaded control. Physiologically adapted cyclical loading (DIN ISO 7206-4) was performed with a hip simulator. After loading both interfaces and the bone cement itself were analysed regarding gaps and cracks in the cement mantle. Significant differences between the stem designs concerning gaps in the metal-bone cement interface and cracks in the cement mantle became apparent. Additionally, a high correlation between gaps in the metal-bone cement interface and cracks within the cement mantle could be proven. Gaps in the metal-bone cement interface but no cracks within the cement mantle were seen in the unloaded specimens. Differences between the unloaded control groups and the cyclical loaded stems regarding the longitudinal extension and width of gaps in the metal-bone cement interface were obvious. The designs of cemented femoral stems have an influence on both the quality of the metal-bone cement contact and the failure rate of the cement mantle. Less interface gaps and less cement defects were found with anatomically formed, collared, well-rounded stem designs without undercuttings.

[Functionality and accuracy of a fluoroscopic navigation system in the placement of the femoral component for hip resurfacing - a cadaver study]. / Funktionalität und Genauigkeit eines fluoroskopischen Navigationssystems zur Implantation von Hüftkopf-Oberflächenersatzprothesen: Eine Anatomiestudie.

INTRODUCTION: Resurfacing arthroplasty represents an alternative method to total hip replacement especially for the young and active patient. The main reasons for early implant failure are mal-positioning of the femoral component and notching of the femoral neck during femoral head preparation. MATERIAL AND METHODS: In the context of a cadaver study of formalin-fixed cadaveric full body specimens 6 DUROM -Hip-Resurfacing prosthesis have been implanted under navigation control. The aim of the study was an evaluation of the functionality and accuracy of the computer-assisted planning and navigation system on the basis of a navigation module library from Surgitaix AG Aachen, Germany. RESULTS: The main angulation error between planning (135.2 +/- 3.6 degrees ) and navigation (136.2 +/- 2.8 degrees ) was 1.9 +/- 1.1 degrees , the main anterior offset error between planning (2.2 +/- 1.3 mm) and navigation (2.7 +/- 2.3 mm) was 1.2 +/- 1.9 mm. The main distance error between planning and navigation was 2.7 +/- 1.3 mm. The mean time for all five planning and navigation steps was 20.2 +/- 2.5 min. Against the background of a acetabular bone-saving approach in all 6 cases the smallest possible femoral component could be implanted. CONCLUSION: The computer-assisted fluoroscopic planning and navigation system for hip resurfacing showed within the scope of this cadaver study first promising results. The system approaches a practicable intraoperative planning with a high accuracy in operative implementation. Nevertheless, the potential benefit has to be evaluated in further clinical studies, especially from the perspective of a possible integration of this navigation system into the clinical work-flow. Further studies should consider a fluoroscopic-assisted range of motion assessment under consideration of an additional cup-module to enhance the postoperative range of motion after hip resurfacing procedures.

[Clinical results after three years use of matrix-associated ACT for the treatment of osteochondral defects of the knee]. / Klinische Drei-Jahres-Ergebnisse der matrixgekoppelten ACT zur Behandlung osteochondraler Defekte am Kniegelenk.

AIM: The aim of this study was to examine the clinical results after the treatment of osteochondral defects of the knee with autologous bone grafting and matrix-supported autologous chondrocyte transplantation (ACT). METHOD: In this study 13 patients with osteochondral defects of the knee (12 femoral, 1 tibial) with OCD ICRS grade IV or ICRS grade IV B were included. The osteochondral defects were reconstructed simultaneously with autologous monocortical cancellous bone cylinders or chips from cancellous bone and matrix-supported autologous chondrocyte transplantation (CaReS). Data were analysed in accordance with the ICRS criteria and the Brittberg score. Patients were followed up to 36 months after the operation. RESULTS: The average age of the patients was 34.9 (16 - 47) years at the time of surgery. The size of the defect was 8.1 (3.8 - 13.5) cm(2). The subjective and objective IKDC scores, the Brittberg and the ICRS function score were statistically significantly improved during the observation time. In one patient the transplantation failed and another patient was not available for the follow-up at 36 months. The objective IKDC score and the ICRS function score increased from 0/13 (0 %) to 11/12 (91.7 %) in categories A and B, or I and II, respectively, after 3 years. At this point of time 83.4 % (10/12) of the patients voted the treatment as excellent or good. The subjective IKDC score improved from 38.4 (+/- 12.7) preoperatively to 66.1 (+/- 17.0) after 3 years. CONCLUSION: The treatment of osteochondral defects of the knee with autologous bone grafting and matrix-supported autologous chondrocyte transplantation shows promising results even for larger defects. But for a final decision about this therapy the present sample size was too small. There is a need for further long-term investigation with a larger number of patients.

[Mechanical bending strength of explanted ex vivo bone cements compared with in vitro samples]. / Mechanische Biegefestigkeiten von explantierten Ex-vivo-Knochenzementen verglichen mit In-vitro-Proben.

INTRODUCTION: The mechanical properties of acrylic bone cements are an important factor in determining an efficient load transmission between prosthesis and bone to guarantee the long-term stability in cemented hip arthroplasty. MATERIAL AND METHODS: Palacos and Refobacin Palacos specimens from 21 aseptically loosened femoral compoments of cemented hip arthroplasties manufactured by the first to third generation cementing technique have been mechanically tested in a standardised four-point bending test (ISO 5833). In vitro manufactured Palacos and Refobacin Palacos specimens served as a control group. The fatigue fracture surfaces were morphologically analysed with light microscopy. RESULTS: Under in vitro conditions manufactured specimens had higher values of bending strength, with the exception of blood contaminated ones, compared to ex vivo specimens. Ex vivo specimens of the second and third generation cementing technique had higher values than specimens of the first generation. CONCLUSIONS: Acrylic bone cements are subjected to a multifactorial material fatigue in vivo. Here, the art and quality of cementing technique is of eminent importance in determining the long-term stability of cemented hip arthroplasty.