ABSTRACT
OBJECTIVE: To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. METHODS: One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), ascorbate oxidase, peroxidase, polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione (TRG) and lipid peroxidation (LPO). RESULTS: The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 µg/mg protein, GST 372.11±5.70 µmol of 1-chloro 2,4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 µg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 µmol/g tissue, TRG 75.27±3.55 µg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 µmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 µmol/g tissue in moderate amount. CONCLUSION: Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.
Subject(s)
Alpinia/chemistry , Antioxidants/analysis , Enzymes/metabolism , Plant Extracts/chemistry , Plant Leaves/chemistry , Catechol Oxidase/metabolism , Lipid PeroxidationABSTRACT
OBJECTIVE: The study was formulated with the objective to assess phytochemical constituents, antibacterial activity and anticancer activity of Alpinia purpurata. METHODS: The leaves of A. purpurata were washed thoroughly by tap water, shade dried and powdered. The plant powder was extracted with successive solvent system. Phytochemical constituents were evaluated, antibacterial activity was carried out by disc diffusion method and anticancer activity of the ethyl acetate leaf extract was evaluated by using 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. RESULTS: The ethyl acetate extract of A. purpurata showed most of the phytochemicals. The extract exhibited antibacterial activity with a zone of inhibition from 5 to 14 mm at various concentrations and the extract showed potential anticancer activity against PA1 ovarian cancer cell line at the 48 h with half maximal inhibitory concentration value of 110.25 µg/mL and exhibited a dose-dependent decrease in cell count for all the concentrations tested. CONCLUSION: The present study scientifically proved that ethyl acetate leaf extract of A. purpurata is a good source of phytoconstituents, showing antibacterial and anticancer activities.
Subject(s)
Alpinia/chemistry , Anti-Bacterial Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Plant Extracts/pharmacology , Cell Line, Tumor , Humans , Plant Extracts/chemistry , Plant Leaves/chemistryABSTRACT
The study was formulated with the objective to assess phytochemical constituents, antibacterial activity and anticancer activity of Alpinia purpurata.
