ABSTRACT
CASE: A 37-year-old man American Society of Anesthesiologists grade 1 patient with lumbar canal stenosis at the L4-L5 level underwent endoscopic decompression. Toward the end of the procedure, the patient developed sudden-onset bradycardia, followed by ventricular arrhythmia and acute pulmonary edema. The patient was successfully managed with resuscitation and supportive management and recovered uneventfully thereafter. A diagnosis of perioperative stress cardiomyopathy was subsequently made after evaluation of the patient. CONCLUSION: The possibility of takotsubo cardiomyopathy should be considered in cases of acute perioperative cardiac decompensation and pulmonary edema in patients undergoing spinal surgery.
Subject(s)
Spinal Stenosis , Takotsubo Cardiomyopathy , Humans , Takotsubo Cardiomyopathy/etiology , Takotsubo Cardiomyopathy/diagnostic imaging , Adult , Male , Spinal Stenosis/surgery , Endoscopy/adverse effects , Lumbar Vertebrae/surgery , Decompression, Surgical/adverse effects , Intraoperative Complications/etiologyABSTRACT
Novel 2,3-dihydrofuran derivatives were synthesized through a tandem Knoevenagel-Michael cyclization in good yield by reacting α-tosyloxy ketone, 5,5-dimethyl-1,3-cyclohexanedione, and various aldehydes in the presence of phthalazine in acetonitrile. These compounds were subjected to inâ vitro antibacterial screening against eight micro-organisms by using diffusion method and also inâ vitro cytotoxicity screening against four human cancerous cell lines by applying MTT assay. Some of the compounds showed impressive activities.
Subject(s)
Anti-Bacterial Agents/pharmacology , Antineoplastic Agents, Phytogenic/pharmacology , Bacteria/drug effects , Furans/pharmacology , Ketones/chemistry , Tosyl Compounds/chemistry , Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/chemistry , Antineoplastic Agents, Phytogenic/chemical synthesis , Antineoplastic Agents, Phytogenic/chemistry , Cell Line, Tumor , Cell Proliferation/drug effects , Cyclization , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Furans/chemical synthesis , Furans/chemistry , Humans , Microbial Sensitivity Tests , Molecular Structure , Structure-Activity RelationshipABSTRACT
Alcaligenes sp. HPC 1271 demonstrated antibacterial activity against multidrug resistant bacteria, Enterobacter sp., resistant to sulfamethoxazole, ampicillin, azithromycin, and tetracycline, as well as against Serratia sp. GMX1, resistant to the same antibiotics with the addition of netilmicin. The cell-free culture supernatant was analyzed for possible antibacterials by HPLC, and the active fraction was further identified by LC-MS. Results suggest the production of tunicamycin, a nucleoside antibiotic. The draft genome of this bacterial isolate was analyzed, and the 4.2 Mb sequence data revealed six secondary metabolite-producing clusters, identified using antiSMASH platform as ectoine, butyrolactone, phosphonate, terpene, polyketides, and nonribosomal peptide synthase (NRPS). Additionally, the draft genome demonstrated homology to the tunicamycin-producing gene cluster and also defined 30 ORFs linked to protein secretion that could also play a role in the antibacterial activity observed. Gene expression analysis demonstrated that both NRPS and dTDP-glucose 4,6-dehydratase gene clusters are functional and could be involved in antibacterial biosynthesis.
Subject(s)
Alcaligenes/metabolism , Antibiosis , Drug Resistance, Multiple, Bacterial , Genome, Bacterial , Alcaligenes/genetics , Alcaligenes/isolation & purification , Amino Acids, Diamino/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Hydro-Lyases/genetics , Hydro-Lyases/metabolism , Open Reading Frames , Organophosphonates/metabolism , Peptide Synthases/metabolism , Polyketides/metabolism , Serratia/drug effects , Terpenes/metabolism , Tunicamycin/genetics , Tunicamycin/metabolismABSTRACT
Chemical investigation on Orthosiphon glabratus afforded 10 compounds, among which a chromenochalcone and a chromene are new. The structures of the new constituents were settled from their 1D- and 2D-NMR spectra. The synthesis of these two compounds and some of their analogues have been accomplished.
